124 results about "Bacterial population" patented technology

The claimed invention provides for new sample preparation methods enabling direct sequencing of PCR products using pyrophosphate sequencing techniques. The PCR products may be specific regions of a genome. The techniques provided in this disclosure allows for SNP (single nucleotide polymorphism) detection, classification, and assessment of individual allelic polymorphisms in one individual or a population of individuals. The results may be used for diagnostic and treatment of patients as well as assessment of viral and bacterial population identification.

A method is provided for the identification of polymorphic markers in a population. The method includes genotypically characterizing a first sample of a population, selecting one or more individuals of the first sample based upon the genotypic characterization, fabricating a microarray with genomic DNA from each individual selected, and genotyping a second sample of the population using each fabricated microarray as a reference, thereby identifying the polymorphic markers in the population. Also provided is a method for the identification of polymorphic markers in a bacterial population. The method includes phenotypically characterizing a first sample of a population, selecting one or more individuals of the first sample based upon the phenotypic characterization, fabricating a microarray with genomic DNA from each individual selected, and genotyping a second sample of the population using each fabricated microarray as a reference, thereby identifying the polymorphic markers in the population. Also provided is a method for identifying unique bits among a plurality of bit strings including providing a plurality of bit strings, wherein each string has the same number and position of bits, and each bit has a value of 0 or 1, generating a graphical representation-including selectable elements-representing the relatedness of the bit strings, making a selection of a first selectable element, making a selection of a second selectable element, and identifying bits that are present in each bit string represented by the first selectable element and absent in each bit string represented by the second selectable element, or vice-versa.

The disclosure provides materials and methods for conferring improved plant traits or benefits on plants. The materials can include a formulation comprising an exogenous endophytic bacterial population, which can be disposed on an exterior surface of a seed or seedling, typically in an amount effective to colonize the plant. The formulations can include at least one member selected from the group consisting of an agriculturally compatible carrier, a tackifier, a microbial stabilizer, a fungicide, an antibacterial agent, an herbicide, a nematicide, an insecticide, a plant growth regulator, a rodenticide, and a nutrient.

The invention discloses an electric heating comprehensive energy system scheduling method based on improved weak robustness optimization. According to the invention, the model description of the powerdemand response (DR) is compared and expanded to the electric heating comprehensive demand response (IDR), and the price type and excitation type comprehensive demand response is modeled; Under the low-carbon background, an electric heating comprehensive energy low-carbon dispatching model containing a combined heat and power generation unit, a pumped storage electric boiler, a heat storage electric boiler and a heat storage device is established, and the electric power constraint condition and the thermal power constraint condition are determined with the minimum comprehensive energy operation cost and the maximum benefit of a carbon emission transaction model as objective functions; Considering the uncertainty of wind power and comprehensive demand response, and the improved weak robustoptimization is utilized to process the uncertainty of the source load side to obtain an improved weak robust low-carbon scheduling model of the electric heating comprehensive energy system; And finally, an improved bacterial population drug tendency algorithm (BCC) is adopted for solving, and the effectiveness of the proposed model and algorithm is verified through examples.

The invention presented herein provides methods and compositions for the prevention and treatment of bacterial infections. The methods are based on the discovery that depletion of bioavailable iron stimulates surface motility in bacteria thus inhibiting the ability of a bacterial population to develop into a biofilm.

The claimed invention provides for new sample preparation methods enabling direct sequencing of PCR products using pyrophosphate sequencing techniques. The PCR products may be specific regions of a genome. The techniques provided in this disclosure allows for SNP (single nucleotide polymorphism) detection, classification, and assessment of individual allelic polymorphisms in one individual or a population of individuals. The results may be used for diagnostic and treatment of patients as well as assessment of viral and bacterial population identification.

Production of alcohol from mixed microbial pool degradable and fermented lignocellulose substance is carried out by crushing lignocellulose substance, soaking with H2SO4, bursting by steam, solid-liquid separating, adding nutritive liquid into solid-phase substance, sterilizing, adding distiller's yeast and cellulose degrading microbe into saccharifying fermentative substrate, degradation saccharifying-fermenting synchronously, distilling and rectifying to obtain final product. It is simple, has short saccharifying and fermenting period, higher raw material utilization rate and more alcohol yield and no feedback inhibition function.

The invention presented herein provides methods and compositions for the prevention and treatment of bacterial infections. The methods are based on the discovery that depletion of bioavailable iron stimulates surface motility in bacteria thus inhibiting the ability of a bacterial population to develop into a biofilm.

Disclosed herein is a method for treating odors in wastewater treatment bodies. The pH of the body is preferably established above about 6 and most preferably above about 7, usually by adding a base to the body. The oxidation reduction potential of the body is established at about −300 mV or higher and more preferably about −200 mV or higher, usually by adding an oxidizing agent such as oxygen or nitrates. After these conditions are established, sulfur oxidizing bacteria are added to the body. These preferably include Paracoccus denitrificans (formerly called Thiosphaera pantotropha) ATTC number 35,512. The sulfur oxidizing bacterial population should be established at levels of at least about 1×104 cfu / mL and more preferably at levels of 1×105 to 1×106 cfu / mL or higher. The increased ORP and pH levels make sulfate reduction thermodynamically unfavorable. Additionally, the presence of an alternative electron acceptor such as oxygen or nitrate allows the sulfur oxidizing bacteria to oxidize organic acids.

Disclosed herein are fire-fighting compositions, and methods thereof, comprising a surfactant and a fraction of a fermentation mixture comprised of yeast exoproteins, where the proteins enhance ability of water to extinguish Class A, Class B and Class K fires, as defined by the National Fire Protection Association. In one embodiment the surfactant is optimized to wet and penetrate into the substrate, for example wood, as in a Class A fire. In a second embodiment, the surfactant is optimized for foaming to provide fire fighting protection in a Class B fire. Further embodiments include methods for using the same protein-surfactant fire fighting composition for both polar (e.g., alcohol) and non-polar combustible liquids. In yet another embodiment, methods are described where the proteins comprise stress shock proteins and where the residual protein-surfactant combination that is not degraded from the fire acts to stimulate resident bacterial populations to accelerate the biodegradation of residual hydrocarbons and surfactants from the composition. In another embodiment, the compositions are free of solvents, fluorine-free as in fluoro surfactants and fluoro-polymers.

The present invention relates to a method of producing a vaccine for the prevention of F. necrophorum bacterial infections, comprising isolating the F. necrophorum bacteria from a bovine species, growing the bacteria in a suitable growth medium for a period equal to between about 10 hours and about 18 hours so as to achieve a bacterial population equal to at least 1x105 CFU / ml, terminating the growth, and using a whole cell culture to form the vaccine. Additionally, the present invention relates to the vaccine comprised of a killed whole cell population of the F. necrophorum bacteria taken from a bovine. The present invention further relates to a method for preventing footrot and liver abscesses caused by F. necrophorum bacteria.

The invention discloses a hybrid intelligent optimization method and belongs to the technical field of artificial intelligence and data mining. A genetic optimization algorithm and a bacterial foraging optimization algorithm are organically combined by the hybrid intelligent optimization method. First, a preliminary superior solution is obtained through the breadth searching capacity of the genetic optimization algorithm and serves as an initial bacterial population in the posterior bacterial foraging algorithm, by fully utilizing chemotaxis, duplicating and dispersing operation of the bacterial foraging algorithm, excellent individuals are generated continuously, and finally the solution converges towards the optimal solution. On the basis of the technical scheme, further improvements are made in the four aspects of genetic selection operators, optimal joint pints, bacterial chemotaxis and duplicating operation. Compared with the prior art, the convergence speed and the convergence precision of an optimal solution set can be improved, and the hybrid intelligent optimization method is more extensive in applicability.

The invention discloses an effluent index online soft measurement prediction method in the urban sewage treatment process. According to the method, firstly an instant learning model structure with correlation as the principle and the recursive partial least-square method as a local model is adopted, and meanwhile, description on uncertainty of the model is performed on the basis of the statistical learning theory. The model can be used for fully reflecting the characteristics such as uncertainty and large lag of the sewage treatment process, the explanatory performance of a prediction result is higher, controllers can adjust process variables such as the aeration rate and the backflow amount of an aeration tank in time, material balance and bacterial population balance are kept, and efficient removal of organic matter is achieved fully.

A denitrification system for denitrified wastewater or nitrified water treatment using a detergent or detergent-like compound comprising available carbon. The system comprises a plurality of interconnected tanks including a mixing tank which feeds detention tanks which in combination provide a detention time period for the effluent. A controller determines the amount of detergent dispensed into the mixing tank in accordance with the measured volume of effluent to be treated. The mixing tank comprises a heater for maintaining the nitrified effluent temperature above 50 degrees F. An optional line filter can be added to the output of the system for further reducing organic nitrogen concentration. An alternate embodiment comprises passing the final denitrified effluent through a pump chamber for pumping of the effluent to a sand filter capable of reducing bacterial populations.

The present invention relates to a method of producing a vaccine for the prevention of <HIL><PDAT>F. necrophorum < / ITALIC><PDAT>bacterial infections, comprising isolating the <HIL><PDAT>F. necrophorum < / ITALIC><PDAT>bacteria from a bovine species, growing the bacteria in a suitable growth medium for a period equal to between about 10 hours and about 18 hours so as to achieve a bacterial population equal to at least 1x10<HIL><5 < / SP><PDAT>CFU / ml, terminating the growth, and using a whole cell culture to form the vaccine. Additionally, the present invention relates to the vaccine comprised of a killed whole cell population of the <HIL><PDAT>F. necrophorum < / ITALIC><PDAT>bacteria taken from a bovine. The present invention further relates to a method for preventing footrot and liver abscesses caused by <HIL><PDAT>F. necrophorum < / ITALIC><PDAT>bacteria.< / PTEXT>

A method of producing a fermentation-based product, such as ethanol, comprises fermenting a sugar-containing medium with yeast in the presence of an aliphatic or aromatic monoaldehyde or dialdehyde, a formaldehyde-releasing compound, or a combination thereof, in an amount sufficient to reduce or control a bacterial population in the sugar-containing medium. The additive may enable reduction or elimination of antibiotics for this purpose, while showing reductions in percent infection, process variability and interference with yeast viability.

In one aspect of the present invention, a method is provided for reducing or eliminating antibiotic resistance in bacteria, comprising exposing the bacteria to a composition comprising a yeast cell wall preparation in an amount effective for reducing or eliminating resistance of the bacteria to at least one antibiotic. In one embodiment, the method comprises exposing the bacteria to the yeast cell wall preparation in an amount effective for eliminating a plasmid conferring resistance to the antibiotic, or for preventing transfer of the plasmid between bacteria. In another aspect, a method is provided for reducing prevalence of antibiotic-resistant bacteria in an animal, comprising administering to the animal a composition comprising a yeast cell wall preparation in an amount effective for reducing or eliminating the presence of an antibiotic-resistant bacterial population in the animal.

The invention discloses a method for adjusting and controlling algal facies and water quality of an aquaculture pond in situ. The method comprises the following steps: a, kieselguhr powder with particle size of 200-1000 mesh is selected, and a compound microbial preparation is selected, wherein the bacterial population in 1 ml of the compound microbial preparation is greater than or equal to 100 million; b, when a base fertilizer and rich water are started to apply in the pond, the kieselguhr powder and the compound microbial preparation both obtained in step a are sprayed in the pond; c, after 15-20 days, the dissolved oxygen concentration and the ammonia concentration in the pond water are maintained at 5-9 mg / L and 0.1-0.4 mg / L respectively, the pond water is sandy beige, and the pond algal face is mainly alga of bacillariophyta observed through a microscope; d, offspring seeds of a to-be-cultivated target are put in the pond. Through the way, the method, disclosed by the invention, can improve the using effect of the microbial preparation commonly used in aquaculture, effectively adjusts and controls algal facies and water quality, reduces occurrences of diseases, and improves cultivation quality and quality of aquaculture products.

Disclosed herein is a method for treating odors in wastewater treatment bodies. The pH of the body is preferably established above about 6 and most preferably above about 7, usually by adding a base to the body. The oxidation reduction potential of the body is established at about -300 mV or higher and more preferably about -200 mV or higher, usually by adding an oxidizing agent such as oxygen or nitrates. After these conditions are established, sulfur oxidizing bacteria are added to the body. These preferably include Paracoccus denitrificans (formerly called Thiosphaera pantotropha) ATTC number 35,512. The sulfur oxidizing bacterial population should be established at levels of at least about 1x10<4 >cfu / mL and more preferably at levels of 1x10<5 >to 1x10<6 >cfu / mL or higher. The increased ORP and pH levels make sulfate reduction thermodynamically unfavorable. Additionally, the presence of an alternative electron acceptor such as oxygen or nitrate allows the sulfur oxidizing bacteria to oxidize organic acids.

The invention relates to a method for recovering at least one metal present within a material, said material possibly including iron, said method including a step of supplying a ferrous ion, a step of supplying a ferric ion, and a step of bioleaching at least one metal present in the material by the ferric ions, each one of the steps being implemented by a particular bacterial population.

The present invention provides improved tests for the detection of methicillin-resistant Staphylococcus aureus. The tests are particularly useful for eliminating false positive results due to the presence of a mixed bacterial population in patient samples.

The invention relates to a method for degrading histamine in aquatic products. The washed aquatic products are immersed in a treating fluid, fished out, drained, put into an insulating bag for high-voltage pulsed electric field treatment, added to a fermentation container and fermented at the controlled temperature of 23 DEG C for 20-35 days; the aquatic products are collected, packaged and put into freezing storage. Histamine produced in the transportation and storage processes of the aquatic products can be remarkably reduced while the specific texture and nutritional ingredients of the aquatic products are retained, growth and metabolism of bacterium colonies producing amino acid decarboxylase activity in the processing process of the aquatic products can be inhibited or prevented better, generation of amino acid decarboxylase is prevented fundamentally, accordingly, generation of histamine is prevented in the whole processing process and is further controlled, and the method has great social and economic benefits.

Disclosed herein are compositions that comprise one or more types of bacteriophage and methods of using such bacteriophages as a prebiotic to promote the growth of beneficial bacteria by decreasing harmful bacterial populations and releasing nutrients into the environment for good bacteria in the digestive system of an individual.

The invention discloses a recovery liquid for VBNC salmonella, a method for preparing the same and a reclaiming method. The recovery liquid consists of 1 portion of serum and 1 to 3 portions of sterile water which are mixed and stirred evenly and are filtered for sterilization through a millipore filter. The reclaiming method comprises the following steps: placing the salmonella in a VBNC into the recovery liquid and adopting an incremental temperature raising mode in pores of a thermal program control circulator, wherein the recovery temperature rise and an incubation time program of the thermal program control circulator are at a temperature of between 5 and 37 DEG C, and the incubation time is between 2 minutes and 1.5 hours; and then inoculating the salmonella in an SS liquid culture medium, and culturing the salmonella in a gas bath shaking table at a speed of between 200 and 220 revolutions per minute. The invention overcomes the defects that the prior art uses a plurality of materials, is inconvenient to get materials and the like; the invention only uses one material to recover the salmonella which is in the VBNC for nearly one year through procedural temperature rise; and few materials are used and the operation is simple and convenient, the concentration of the recovered salmonella can reach 10<8> CFU per milliliter in 12 hours, and each recovery index (time, bacterial population and the like) is greatly improved compared with that of the prior art.

The disclosure provides materials and methods for conferring improved plant traits or benefits on plants. The materials can include a formulation comprising an exogenous endophytic bacterial population, which can be disposed on an exterior surface of a seed or seedling, typically in an amount effective to colonize the plant. The formulations can include at least one member selected from the group consisting of an agriculturally compatible carrier, a tackifier, a microbial stabilizer, a fungicide, an antibacterial agent, an herbicide, a nematicide, an insecticide, a plant growth regulator, a rodenticide, and a nutrient.

Method of modeling the biodegradation of hydrocarbons trapped in an oil reservoir or trap through the action of the bacterial population in an underlying aquifer. From data relative to the reservoir studied, concerning the form and the height of the reservoir, the physical characteristics of the porous medium, the thickness of the transition zone between the hydrocarbons and the water, the composition of the hydrocarbons, of the flow of electron acceptors entering the reservoir and data relative to the bacterial population in the aquifer, modeling is performed by discretizing the reservoir by means of a grid wherein the height of each grid cell is the thickness of the transition zone, and the variation, over the height of the reservoir, of the proportion in heavy fractions of hydrocarbons under the effect of the biodegradation is determined by iterative adjustment in each grid cell of the bacterial population to the amount of hydrocarbons available, the pore space available, the amount of electron acceptors present in the reservoir and the degradation capacities of said population. Applications: determination of the composition of oils in a reservoir and notably of the location of the heaviest fractions.

The present invention relates to chimeric polypeptides comprising a first portion, which comprises a bacteriocin cell wall-binding domain (CBD) and a second portion, which comprises an enzymatic active domain (EAD) selected from the lytic domain of a bacteriophage lysin, a bacteriocin and a bacterial autolysin. Provided are such chimeric polypeptides and variants and fragments thereof, nucleic acids encoding the same, vectors carrying such nucleic acids and host cells transformed or transfected with such vectors. The chimeric polypeptides of the present invention are useful for the reduction of certain bacterial populations, including methods and compositions for the treatment of various bacterial infections. For example, chimeric polypeptides of the present invention have been shown to effectively kill various bacteria, including methicillin-resistant Staphylococcus aureus (MRSA), as well as other human pathogens. Thus, provided are compositions comprising chimeric polypeptides according to the present invention, variants or fragments thereof, and the use of such compositions in prophylaxis or therapy of bacterial diseases, bacterial infections or bacterial colonisations.

Embodiments of the invention include methods and / or compositions for analysis of samples for C. difficile infection to determine whether or not an individual is at risk for having recurrent C. difficile infection or a CDI misdiagnosis. Methods include characterization of microflora composition from the gut, wherein alterations of the microflora gut composition are indicative of recurrence of infection. Methods include analysis of nucleic acids from the gut, such as 16S rRNA as being identifying of a particular bacteria in the analysis of bacterial populations of the gut.

The invention discloses a low-histamine fish product processing method. The low-histamine fish product processing method comprises the following steps: selecting a fish with relatively high integrity and freshness as a frozen raw fish, and freezing the fish; thawing the frozen raw fish by ozone-containing flowing water with a certain flow velocity, wherein in the process of thawing, the temperature of water in a thawing tank does not exceed 15 DEG C and the processing time does not exceed 45 minutes; controlling the indoor ambient temperature of a processing workshop to be below 21 DEG C and the relative air humidity to be less than 70%; and sterilizing the raw fish, rapidly cooling the raw fish and taking fish meat, mixing the fish meat with a certain amount of flavoring liquid, and thencanning the fish meat to obtain a finished product. The low-histamine fish product processing method has the advantages that: the growth and metabolism of a bacterial population with an activity of generating amino acid decarboxylase in the aquatic product canning process can be inhibited or eliminated better; and the generation of the amino acid decarboxylase is eliminated from a source so as tofurther control the generation of histamine.