The invention discloses a kit for detecting a human optimal partner potential genotype and a method thereof. The method adopts multiple PCR amplification and electrophoresis methods to analyze and identify allele polymorphisms (SNP) of seven genes: SNAP25, CLOCK, OXTR, DRD2, CADM2, FOXP2 and KATNAL2. The method comprises the following steps: a) collecting exfoliated cells in the oral cavity of a testee and storing the collected exfoliated cells in a collection card or performing DNA nucleic acid extraction on a blood sample; b) adding a saliva collection card sample or an extracted DNA sample of a testee into a reaction system, and carrying out PCR amplification; c) running an amplification program; and d) carrying out electrophoretic analysis on the amplification product, and interpreting according to a peak pattern graph. According to the invention, SNP of a plurality of genes related to a testee can be synchronously detected, and simplicity, high efficiency and specificity of detection are realized. Through the analysis, a reference is provided for whether the testee has the potential of the best partner or not.