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Kit for detecting individual skin genes and use method of kit

A kit and gene technology, applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of expensive next-generation sequencing platform, low popularity, and immature application of SNP detection technology

Pending Publication Date: 2021-06-18
NINGBO HEALTH GENE TECHNOLOGIES CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the next-generation sequencing platform is expensive and low in popularity, and its clinical application as a SNP detection technology is not mature enough
[0011] Due to the obvious limitations of the above technologies, it is difficult to apply multiple genetic testing to detect individual skin quality
At present, there is no report on the detection of individual skin quality genes based on multiplex PCR and CE separation technology in China

Method used

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  • Kit for detecting individual skin genes and use method of kit
  • Kit for detecting individual skin genes and use method of kit
  • Kit for detecting individual skin genes and use method of kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] (1) Selected sample: DNA / RNA-free water.

[0047] (2) System configuration

[0048]According to the instructions, prepare the primer Primer Mix and PCR reaction solution on ice according to the ratio of the instructions to configure the reaction system. After vortexing and mixing, centrifuge with a centrifuge, and then use a pipette tip to mix and distribute.

[0049] (3) Add samples

[0050] According to the instructions, use a pipette to take the corresponding volume of DNA / RNA-free water and add it to the reaction system that has been aliquoted.

[0051] (4) Amplification program

[0052] The amplification program on the PCR instrument is shown in Table 2.

[0053] (5) The amplified product is detected on the 3500DX genetic analyzer

[0054] The sample loading mixture is composed of deionized formamide and molecular weight internal standard (Size-500) in the system {(1μL Size-500+12μL deionized formamide)×(injection number)}. Mix 9 μL of the loading mixture with...

Embodiment 2

[0060] (1) Selected samples: exfoliated cell samples from the oral cavity wall of subject 1 and subject 2.

[0061] (2) Sample collection

[0062] Collection type: Exfoliated cells from the inner wall of the oral cavity.

[0063] Collection method: Use the saliva collection stick to wipe back and forth on the inner wall of the oral cavity 4 times, continue to wipe back and forth on the inner wall of the oral cavity 4 times with the reverse side of the saliva collection stick, take out the saliva collection stick, and press repeatedly on the saliva sample collection card to collect the saliva inner wall cells Transfer to the saliva sample collection card, and dry the collected saliva sample collection card in a pollution-free area.

[0064] Valid sample: The area where the pink area of ​​the saliva sample collection card changes to light pink or white is the valid saliva sample area.

[0065] Sample selection method: use Dubbo plastic puncher (1.0mm) for manual punching and s...

Embodiment 3

[0085] (1) Selected sample: the blood sample of subject 2.

[0086] (2) Sample type: blood sample.

[0087] (3) DNA extraction: DNA extraction is performed on the blood sample using a nucleic acid extraction instrument.

[0088] (4) System configuration

[0089] According to the instructions, prepare the primer Primer Mix and PCR reaction solution on ice according to the ratio of the instructions to configure the reaction system. After vortexing and mixing, centrifuge with a centrifuge, and then use a pipette tip to mix and distribute.

[0090] (5) Adding samples: According to the instructions, take a certain amount of extracted DNA samples with a pipette and add them to the reaction system.

[0091] (6) Amplification program

[0092] The amplification program on the PCR instrument is shown in Table 2.

[0093] (7) The amplified product is detected on the 3500DX genetic analyzer

[0094] The sample loading mixture is composed of deionized formamide and molecular weight in...

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Abstract

The invention discloses a kit for detecting individual skin genes and a use method of the kit. Allele polymorphisms (SNPs) of four genes, namely MMP1, MMP3, MMP9 and AQP3 are analyzed and identified by adopting a multiple PCR amplification and electrophoresis method. The method comprises the following steps: a) collecting cast-off cells in the oral cavity of a testee and storing the cast-off cells in a collection card or performing DNA nucleic acid extraction on a blood sample; b) adding a saliva collection card sample or an extracted DNA sample of the testee into a reaction system, and carrying out PCR amplification; c) running an amplification program; and d) carrying out electrophoretic analysis on the amplification product, and carrying out interpretation according to a peak pattern graph. According to the invention, the SNP of a plurality of genes related to the testee can be synchronously detected, and simplicity, high efficiency and specificity of detection are realized; and the reference is provided for the skin type of the testee through the analysis.

Description

technical field [0001] The invention relates to the field of gene detection, in particular to a kit for detecting individual skin quality genes and a use method thereof. Background technique [0002] With the development of society, people begin to pay more and more attention to the quality of their skin to make themselves look younger and more energetic. Recent studies have shown that up to 60% of the variation in skin texture between individuals can be attributed to genetic factors, while the remaining 40% is due to non-genetic factors such as light exposure, climate, etc. Studies in genomics and bioinformatics have shown that certain single nucleotide polymorphisms (Single Nucleotide polymorphism, SNP) are associated with individual skin texture. SNP refers to DNA sequence polymorphism caused by single nucleotide variation at the genome level, including base conversion, transversion, deletion and insertion. More than 80% of the polymorphisms in the human genome are sing...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12Q1/6858
CPCC12Q1/6888C12Q1/6858C12Q2600/156C12Q2600/166C12Q2531/113C12Q2537/143
Inventor 朱浩威徐智孔咪咪余丁吴勇
Owner NINGBO HEALTH GENE TECHNOLOGIES CO LTD
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