The invention discloses an SSR (simples sequence repeats) and
InDel (
insertion / deletion)
molecular marker primer linked with
brassica campestris orange head
gene Br-or and an application of the
molecular marker primer. The marker primer is obtained by the steps of: based on the
genome DNA (deoxyribonucleic acid) of orange
brassica campestris and normal white
brassica campestris and F2S4 separation group as a template, carrying out polymorphism primer screening to orange head and white head
single strain DNA mixed tank in F2S4 by using 480 pairs of SSR and
InDel primer pairs, then analyzing
single strain of F2S4 group, and screening the
molecular marker linked with the brassica campestris orange head
gene Br-or to finally obtain fifteen Br-SSR and three Br-
InDel molecular markers linked with the Br-or
gene, wherein a molecular genetic map of the brassica campestris orange head gene Br-or is created on the ninth
link group (A09). According to the
link analysis, the
genetic distance of two markers most tightly linked with both sides of the Br-or gene are 0.11cM and 0.79cM; and the molecular marker primer has the advantages of being convenient for detection, stable in amplification, and high in
repeatability and accuracy, and thus a basis is provided to the molecule assistant
selective breeding of brassica campestris orange head character and the breeding process is accelerated.