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Method for identifying authenticity of sweet potato variety by using inter-simple sequence repeat (ISSR) molecular marker method

A technology of authenticity and sweet potato, which is applied in the field of authenticity identification of sweet potato varieties, and achieves the effects of good repeatability and stability, and easy identification.

Active Publication Date: 2014-01-01
INST OF FOOD CROPS HUBEI ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In sweet potato, the application of ISSR molecular markers to analyze the genetic diversity of sweet potato germplasm resources has been reported, but the method of using ISSR molecular markers to identify the authenticity of sweet potato varieties has not been reported

Method used

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  • Method for identifying authenticity of sweet potato variety by using inter-simple sequence repeat (ISSR) molecular marker method
  • Method for identifying authenticity of sweet potato variety by using inter-simple sequence repeat (ISSR) molecular marker method
  • Method for identifying authenticity of sweet potato variety by using inter-simple sequence repeat (ISSR) molecular marker method

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Experimental program
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Embodiment 1

[0066] Embodiment 1, be used for the construction of the ISSR fingerprint spectrum table of sweet potato variety identification

[0067] 1. DNA extraction

[0068] Using the leaves grown from 16 different sweet potato varieties shown in Table 1 as materials, DNA was extracted by SDS method, and the purity, quality and integrity of the total DNA of each variety of sweet potato were detected by 1% agarose gel electrophoresis. The absorbance value of the extracted DNA at 260nm and 280nm was measured with an ultraviolet spectrophotometer to determine the purity and concentration of the DNA. The DNA of each variety of sweet potato was diluted to 50-100 ng / μL, and stored at -20°C for later use.

[0069] 2. Primer synthesis

[0070] Synthesize the following three primers:

[0071] HBAAS-001: the sequence is 5'-ACACACACACACACACCG-3' (SEQ ID No.1)

[0072] HBAAS-815: the sequence is 5'-CTCTCTCTCTCCTCTCTG-3' (SEQ ID No.2)

[0073] HBAAS-825: the sequence is 5'-ACACACACACACACACT-3' ...

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Abstract

The invention discloses a method for identifying authenticity of sweet potato variety by using an inter-simple sequence repeat (ISSR) molecular marker method. A disclosed primer group used for identifying the authenticity of the sweet potato variety in an assisted mode consists of a primer HBAAS-001, a primer HBAAS-815 and a primer HBAAS-825, wherein the nucleotide sequence of the primer HBAAS-001 is shown as SEQ ID No:1, the nucleotide sequence of the primer HBAAS-815 is shown as SEQ ID No:2, and the nucleotide sequence of the primer HBAAS-825 is shown as SEQ ID No:3. An ISSR fingerprint spectrum of a single primer of the sweet potato variety is successfully constructed according to an ISSR molecular marker, so that the method for identifying the authenticity of the sweet potato variety is simple, convenient, rapid, high in repeatability and high in stability, and a solid foundation is laid for allowing an ISSR molecular marker technology to be widely applied to identifying the authenticity of the sweet potato variety.

Description

technical field [0001] The invention relates to a method for identifying the authenticity of sweet potato varieties by using the ISSR molecular marker method. Background technique [0002] Sweet potato is an important food, feed, industrial raw material and energy crop. In recent years, with the increasingly severe global oil supply situation, the development and utilization of biomass energy has become increasingly important. Sweet potato has a high starch yield per unit area and is considered to be an ideal raw material for the production of fuel ethanol. As a new type of energy plant, sweet potato has huge market potential and will play an important role in my country's energy security. [0003] The authenticity and purity of sweet potato seed is an important index to measure the quality of seed potato, which directly affects the yield and quality of sweet potato. How to quickly and accurately identify the authenticity of sweet potato seed potatoes in production is a p...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/156
Inventor 杨新笋王连军苏文瑾雷剑柴沙沙
Owner INST OF FOOD CROPS HUBEI ACAD OF AGRI SCI
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