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The est-ssr markers and primers used to distinguish different ecotypes of cloves

A technology of cloves and primer pairs, applied in biochemical equipment and methods, measurement/testing of microorganisms, resistance to vector-borne diseases, etc., can solve problems such as time-consuming, labor-intensive, low efficiency, and complicated experimental procedures

Active Publication Date: 2022-06-03
BEIJING UNIV OF AGRI
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

gSSR is located in the coding region and non-coding region of the genome, and the development of markers is mainly based on the method of gene library construction (including SSR enrichment library), and the experimental process is complicated, time-consuming and labor-intensive, and the efficiency is low

Method used

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  • The est-ssr markers and primers used to distinguish different ecotypes of cloves
  • The est-ssr markers and primers used to distinguish different ecotypes of cloves

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Embodiment 1

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Abstract

The invention discloses EST-SSR markers and primers used for distinguishing different ecotypes of cloves. The EST-SSR marker for distinguishing eco-type cloves disclosed by the present invention is obtained by PCR amplification using a primer set composed of 50 single-stranded DNAs shown in sequence 1-50 in the sequence listing. The primer sets of the present invention can be used to identify allelic polymorphisms, identify identical or related plants, differentiate plants and study genetic diversity in populations. The primer set and EST‑SSR marker of the present invention have good application in Syringa, can be used to distinguish different ecotypes of Syringa, and provide convenience for molecular marker-assisted breeding of Syringa and its close relatives.

Description

EST‑SSR markers and primers used to distinguish different ecotypes of cloves technical field The invention belongs to the field of biotechnology, and more specifically, the present invention relates to a method for distinguishing different ecotype cloves. EST‑SSR markers and primers used. Background technique Syringa (Syringa) is a general term for perennial woody plants of the family Oleaceae (Oleaceae) syringa (Syringa). China has a cultivation history of more than 1,000 years and is a precious flower in China. There are about 27 species of cloves in the world, and Chinese cloves There are about 22 species of plants, of which 18 are endemic. The distribution of cloves in China is mainly concentrated in the southwest, northwest, north and Northeast and other regions, including Sichuan, Yunnan and Tibet, there are 13 kinds of cloves that are naturally distributed, and they are also the most endemic species of cloves in my country. many places. Lilac is famous fo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156C12Q2600/13Y02A50/30
Inventor 吴静冷平生郑健胡增辉杨云尧何芮庆
Owner BEIJING UNIV OF AGRI
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