EST-SSR core primer group developed on basis of transcriptome sequence of towel gourd and application thereof

Abstract

The invention discloses an EST-SSR core primer group developed on the basis of the transcriptome sequence of towel gourd. The EST-SSR core primer group comprises 50 pairs of primers with nucleotide sequences as shown in SEQ ID No. 1-100 in a sequence table. The 50 pairs of EST-SSR core primers have the advantages of abundant polymorphism, stable amplification, good repeatability, convenience in statistics, etc.; meanwhile, since the primers are originated from the expressed gene of towel gourd and can reflect variation of functional sequences in a genome, so germplasm genetic diversity of towel gourd can be better distinguished. The core primer group can be used in fields like identification of the variety of towel gourd, genetic genealogical analysis of varieties and analysis of germplasm genetic diversity, is beneficial for protection of legal interests of breeders, producers and consumers and promotes sound development of genetic breeding and production of towel gourd.

Description

technical field [0001] The invention relates to an EST-SSR core primer set, in particular to a set of EST-SSR core primer sets developed based on loofah transcriptome sequences and applications thereof. Background technique [0002] Luffa is an annual climbing herb of Cucurbitaceae (Cucurbitaceae) Luffa spp. As an important vegetable crop, it has a wide planting area in the north and south of my country. In addition to being a vegetable, loofah is also an important medicinal plant. Modern pharmacology has proved that loofah contains a variety of active ingredients such as alkaloids, flavonoids, sterols, and glycosides, which have antifungal, antibacterial, and anti-inflammatory effects. Recent findings have demonstrated that loofah seeds contain ribosome-inactivating proteins that can inhibit the growth of the HIV virus and are therefore a potential drug for the treatment of AIDS. [0003] In recent years, as people pay more and more attention to nutrition and health care,...

AI Technical Summary

Problems solved by technology

Due to the weak basic theoretical research on loofah, there are currently two prominent problems in loofah breeding and variety protection: 1. How to accurately identify the genetic diversity of loofah germplasm resources, so as to efficiently guide breeders to select parents; 2. How to identify loofah varieties accurately and quickly, so as to better promote the development of loofah variety approval, variety protection, identification of true and false varieties, and settlement of property rights disputes of varieties

At present, loofah has no specific molecular markers developed based on genome or transcriptome sequences. Therefore, the development of loofah EST-SSR core primer set has become an urgent problem to be solved in the field of loofah breeding, production and variety protection.

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