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Sorbus pohuashanensis EST-SSR markers, primer pairs thereof and application

A primer pair and labeling technology, applied in the field of molecular biology, can solve problems such as being limited to isozymes, and achieve the effects of enriching basic genetic information, clear bands, and high polymorphism.

Inactive Publication Date: 2017-08-11
BEIJING UNIV OF AGRI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, most of the current studies on rowan tree focus on natural population regeneration, population mating system, geographic provenance variation in seedling shape, artificial breeding technology, and introduction and domestication. Enzyme and other biochemical labeling technology

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  • Sorbus pohuashanensis EST-SSR markers, primer pairs thereof and application
  • Sorbus pohuashanensis EST-SSR markers, primer pairs thereof and application
  • Sorbus pohuashanensis EST-SSR markers, primer pairs thereof and application

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Embodiment Construction

[0035] Embodiments of the present invention will be described in detail below in conjunction with examples, but those skilled in the art will understand that the following examples are only for illustrating the present invention, and should not be considered as limiting the scope of the present invention. Those who do not indicate the specific conditions in the examples are carried out according to the conventional conditions or the conditions suggested by the manufacturer. The reagents or instruments used were not indicated by the manufacturer, and they were all conventional products that could be purchased from the market.

[0036] 1. Materials and methods

[0037] 1.1 Transcriptome data sources

[0038] The transcriptome data of the Sorbus tree came from the results of Illumina high-throughput sequencing of Sorbus tree leaves by the inventor's research group in 2014. The leaves of three 5-year-old Sorbus trees were used for sequencing, RNA was extracted, and Shanghai Boha...

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Abstract

The invention relates to the field of molecular biology, in particular to sorbus pohuashanensis EST-SSR markers, primers thereof and application. The primer pairs of the molecular markers can be used for identifying allele polymorphism and same or relevant plants, distinguishing the plants and researching the genetic diversity of populations. The molecular markers can be also used for heredity and phenotype research by adopting a statistical method, for example, linkage analysis, QTL positioning, genealogical confirmation, genetic polymorphism research of populations, and chain imbalance. The information can be used for identification of specific traits of plants and / or construction of new varieties of molecular identity cards.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to a rowan tree EST-SSR marker, a pair of primers and applications thereof. Background technique [0002] Simple sequence repeat (SSR), also known as microsatellite marker, is a type of DNA sequence composed of tandem repeating units of 1 to 6 nucleotides. SSR markers have the advantages of high polymorphism, co-dominance, reproducibility and high stability. They are currently one of the most valuable molecular markers and are widely used in the fields of human medicine, animals, plants and microorganisms. However, the species-specificity of SSR markers limits the generality of its primers to a certain extent. For species with unknown gene sequences, the development of SSR markers is more difficult. The traditional SSR marker development is mainly based on gene library construction method (including SSR enrichment library), the experimental process is complicated, time-consuming an...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/686C12Q1/6895C12Q2600/156C12Q2525/151
Inventor 郑健关雪莲胡增辉冷平生窦德泉张克中
Owner BEIJING UNIV OF AGRI
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