A research method of intestinal flora based on fecal filtration and transplantation

Abstract

The invention discloses a method for researching the interaction between drugs and intestinal flora based on feces filtration and transplantation. The method includes the following steps: 1. Preparation of experimental animals for fecal bacteria transplantation recipients: selection of experimental animals for fecal bacteria transplantation recipients Germ-free animals or germ-free animals create a sterile or low-bacteria intestinal environment, which is conducive to the colonization of foreign microorganisms. 2. Preparation of experimental animals for fecal microbiota transplantation. Donor animals are subjected to disease model building and drug gavage treatment, and positive and negative experimental controls for drug efficacy are established. 3 Recipient experimental animals received filtered and non-filtered fecal bacteria transplantation from donor experimental animals. This method is based on the classic intestinal microbiota research method of fecal microbiota transplantation, and is simple and easy to implement. It excludes the direct impact of drugs on the intestinal tract and focuses on the drug effects mediated by the microbiota. Interactions provide a neat way to do this.

Description

technical field [0001] The invention relates to a step-by-step processing method for animal experiments in the research of medical intestinal flora, in particular to a research method for intestinal flora based on feces filtration and transplantation. Background technique [0002] Intestinal flora is a complex and huge group. In a healthy adult, the total weight of intestinal flora can reach about 1-2 kg, and the number of cells contained in it can reach as high as 10 13 ~10 14 One, which is about 10 times the number of human cells, and the number of intestinal microbial coding genomes is about 100 times the number of human coding genes, so it is also called the "second genome". [0003] Intestinal flora varies as high as 80% to 90% among individuals, mainly due to differences at the level of bacteria. In addition, the structure of intestinal flora will be affected by many factors, including host genetic factors, geographical conditions, eating habits, health status, drug ...

AI Technical Summary

Problems solved by technology

Biochips can only verify known species, obtain information on microbial diversity by detecting probes immobilized on the chip, and judge the abundance of microorganisms by the strength of the signal, but the results are inaccurate

Correlation analysis usually needs to follow the research ideas of Koch’s law, and return single or several microbial populations to animal models to prove the reliability of the correlation. There is a possibility that microorganisms are not easy to isolate and culture, and it is impossible to observe the influence of overall changes in the bacterial population on the model.

[0010] In addition, the above-mentioned research methods can only study one or several types of intestinal bacteria, or belong to correlation analysis, and cannot directly clarify the effect and mechanism of the joint action of various bacterial groups that actually occur in the body

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