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Preparation method and application of fecal microbiota liquid and preparation method and application of fecal microbiota freeze-dried powder

A technology of freeze-dried powder and fecal bacteria, which is applied in the field of preparation of fecal bacteria liquid and its freeze-dried powder, can solve the problems of large differences in the performance of treatment effects, the range of indications and effects of fecal bacteria are not determined, and achieve the reduction of fecal bacteria. Effects of toxicity, ease of operation

Inactive Publication Date: 2021-03-09
广东南芯医疗科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the scope of indications and effects of fecal bacteria have not been determined, and the performance of treatments other than CDI varies greatly

Method used

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  • Preparation method and application of fecal microbiota liquid and preparation method and application of fecal microbiota freeze-dried powder
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  • Preparation method and application of fecal microbiota liquid and preparation method and application of fecal microbiota freeze-dried powder

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A preparation method of feces bacteria liquid and freeze-dried powder thereof, the specific steps are as follows:

[0027] (1) Preparation of original bacterial liquid: Soak 100g of fresh feces samples from healthy donors in 500mL of 0.9% sterile NaCl solution for 5min, transfer to a stirrer at 500r / min, homogenize for 10min, pass the liquid step by step through a diameter of 2.0mm , 1.0mm, 0.5mm and 0.25mm stainless steel filter to remove food residues and small particles to obtain the original bacterial liquid.

[0028] (2) Centrifugation and washing: Centrifuge the original bacterial solution at 8000g for 15min, discard the supernatant; add 2 times normal saline to the precipitate, mix well, then centrifuge at 8000g for 15min, discard the supernatant; repeat washing 5 times;

[0029] (3) Preparation of feces bacteria liquid: precipitate after washing, add 10% trehalose solution, mix evenly, and obtain feces bacteria liquid;

[0030] (4) Preparation of fecal bacteria...

Embodiment 2

[0052] A preparation method of feces bacteria liquid and freeze-dried powder thereof, the specific steps are as follows:

[0053] (1) Preparation of original bacterial liquid: Soak 50g of fresh feces samples from healthy donors in 200mL of 0.9% sterile NaCl solution for 8min, transfer to a mixer at 1000r / min, homogenize for 2min, pass the liquid step by step through a diameter of 2.0mm , 1.0mm, 0.5mm and 0.25mm stainless steel filter to remove food residues and small particles to obtain the original bacterial liquid.

[0054] (2) Centrifugation and washing: Centrifuge the original bacterial solution at 3000g for 5min, discard the supernatant; add 4 times normal saline to the precipitate, mix evenly, centrifuge at 3000g for 5min, discard the supernatant; repeat washing once;

[0055] (3) Preparation of feces bacteria liquid: precipitate after washing, add 10% trehalose solution, mix evenly, and obtain feces bacteria liquid;

[0056] (4) Preparation of fecal bacteria freeze-dri...

Embodiment 3

[0066] A preparation method of feces bacteria liquid and freeze-dried powder thereof, the specific steps are as follows:

[0067] (1) Preparation of original bacterial liquid: Soak 200g of fresh feces samples from healthy donors in 1000mL of 0.9% sterile NaCl solution for 10min, transfer to a stirrer at 2000r / min, homogenize for 5min, pass the liquid step by step through a diameter of 2.0mm , 1.0mm, 0.5mm and 0.25mm stainless steel filter to remove food residues and small particles to obtain the original bacterial liquid.

[0068] (2) Centrifugation and washing: centrifuge the original bacterial solution at 6000g for 10min, discard the supernatant; add 8 times normal saline to the precipitate, mix well, then centrifuge at 6000g for 10min, discard the supernatant; repeat washing 3 times;

[0069] (3) Preparation of feces bacteria liquid: precipitate after washing, add 10% trehalose solution, mix evenly, and obtain feces bacteria liquid;

[0070] (4) Preparation of fecal bacter...

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Abstract

The invention discloses a preparation method and application of fecal microbiota liquid and a preparation method and application of fecal microbiota freeze-dried powder, and belongs to the technical field of fecal microbiota transplantation. The preparation method of the fecal microbiota liquid and the preparation method of the fecal microbiota freeze-dried powder comprise the following steps: preparing original microbiota liquid; performing centrifuging and washing; preparing the fecal microbiota liquid; and preparing the fecal microbiota liquid freeze-dried powder. The fecal microbiota liquid and the freeze-dried powder thereof prepared by the preparation method disclosed by the invention are used for preparing medicines for treating ulcerative colitis. The fecal microbiota freeze-driedpowder prepared by the invention is concentrated in flora and can be stored for a long time at low temperature. Through washing treatment, the toxicity of fecal microbiota is reduced or removed. A freeze-dried powder technology is adopted, so that implementation of flora transplantation is facilitated, and the operation is convenient.

Description

technical field [0001] The invention relates to the technical field of fecal bacteria transplantation, in particular to a preparation method and application of a fecal bacteria liquid and freeze-dried powder thereof. Background technique [0002] Fecal microbiota transplantation (FMT) refers to the bacterial flora and natural antibacterial substances isolated from the feces of healthy people, which are transplanted to the In the patient's digestive tract, it can rebuild the balance of intestinal flora, repair the intestinal mucosal barrier, and realize the treatment of specific intestinal and extraintestinal diseases. [0003] The safety of fecal microbiota transplantation is a very important but unresolved issue. The short-term adverse events of FMT mainly include fever, diarrhea, abdominal pain, and abdominal distension. Stool samples must not contain relevant pathogenic bacteria, parasites, drug-resistant bacteria, etc. At the same time, during the preparation process,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02C12N1/04A61K35/74A61K9/19A61P1/00A61P1/04
CPCC12N1/20C12N1/02C12N1/04A61K35/74A61K9/19A61P1/00A61P1/04
Inventor 张召马燕郑康帝施婉陈昆义陈涛
Owner 广东南芯医疗科技有限公司
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