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77 results about "Potato virus Y" patented technology
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Potato virus Y (PVY) is a plant pathogenic virus of the family Potyviridae, and one of the most important plant viruses affecting potato production. PVY infection of potato plants results in a variety of symptoms depending on the viral strain. The mildest of these symptoms is production loss, but the most detrimental is 'potato tuber necrotic ringspot disease' (PTNRD). Necrotic ringspots render potatoes unmarketable and can therefore result in a significant loss of income. PVY is transmissible by aphid vectors but may also remain dormant in seed potatoes. This means that using the same line of potato for production of seed potatoes for several consecutive generations will lead to a progressive increase in viral load and subsequent loss of crop.
Ferulic acid and ferulic acid derivative anti-phytoviral agents
ActiveCN102090412AExcellent anti-plant virus activityEffectively prevent and treat viral diseasesBiocideOrganic chemistryNicotiana tabacumTobacco mosaic virus
The invention relates to a ferulic acid shown in a general formula (I) and the application of derivatives thereof to pesticides. When used as novel anti-phytoviral agents, the derivatives can well inhibit tobacco mosaic viruses, pepper viruses, tomato viruses, sweet potato viruses, potato viruses, melon viruses, maize dwarf mosaic viruses and the like, and effectively prevent and control the virus diseases of various crops such as tobacco, peppers, tomatoes, melons, grains, vegetables, beans and the like, and are particularly suitable for preventing and controlling tobacco mosaic. Meanings of each group are shown in specifications.
Owner:NANKAI UNIV
Tobacco virus-resisting RNAi carrier
The invention belongs to the field of biotechnology, and provides an RNAi vector pGEM-PCTRi capable of mediating the resistance to tobacco viral diseases in China. Analyzing the genome characteristics of main virus groups in China, which comprise Potato Virus Y (PVY), Cucumber Mosaic Virus (CMV) and Tobacco Mosaic Virus (TMV) and then selecting three segments of conserved effective sequences capable of inducing RNAi in PVY CP, CMV RNA1 and TMV CP; and integrating the three segments in a tabling manner and constructing an inverted repeat sequence so as to obtain an RNAi vector pGEM-PCTRi. Massproduction of dsRNA can be realized in escherichia coli by utilizing the vector, and the resistance of tobacco to PVY, CMV and TMV can be induced to generate after the tobacco treatment. The invention has the potential of being applicable to the prevention of viral diseases for tobacco farmland production, and endowed with the advantages of broad spectrum, high efficiency, no reference to transgenosis, and the like.
Owner:SHANDONG AGRICULTURAL UNIVERSITY +1
Tobacco recessive PVY (Potato Virus Y) resistance gene eIF4E-1 and application thereof
InactiveCN103820465AImprove disease resistancePlant peptidesFermentationNicotiana tabacumAgricultural science
The invention discloses a tobacco recessive PVY (Potato Virus Y) resistance gene eIF4E-1 and application thereof. The tobacco recessive PVY resistance gene eIF4E-1 comprises the DNA (Deoxyribonucleic Acid) fragment of an eIF4E-1 gene coding region, wherein the DNA fragment is obtained by PCR (Polymerase Chain Reaction) amplification of a specific primer to a primer 4E-1F (SEQIDNO.3) and a primer 4E-1R (SEQIDNO.4); a nucleotide sequence SEQIDNO.1 of a tobacco PVY resistance gene eIF4E-1 coding region and the amino acid sequence SEQIDNO.2 of protein coded by the tobacco PVY resistance gene are obtained first; the eIF4E-1 gene is a recessive disease resistance gene; the eIF4E-1 gene of infected tobacco is lost by a conventional breeding means, so that the non-GMO (Genetically Modified Organism) disease resistance tobacco is obtained; an interference vector plasmid is built by using the eIF4E-1 gene and converted to the infected tobacco; expression of the eIF4E-1 gene of the infected tobacco is inhibited, so that genetically-modified tobacco with obviously improved disease resistance is obtained; the gene has an important application prospect in cultivating the PVY viral disease resistance tobacco.
Owner:YUNNAN ACAD OF TOBACCO AGRI SCI
Potato virus-free seedling tissue culture propagation method
InactiveCN103535283ALow costReproduce fastHorticulture methodsPlant tissue culturePotato virus YSeedling
The invention discloses a potato virus-free seedling tissue culture propagation method which relates to the propagation technology of potatoes and belongs to the technical filed of agriculture planting. The method is characterized by comprising the following steps: performing fine selection, performing accelerating germination, performing seed airing, and inoculating aired seeds in a germination culture medium for cultivation and growth, wherein the accelerating germination and sowing are performed at the temperature being 15-20 DEG C; the method has the beneficial effects that compared with a formula based on an MS, a formula in the method is small in quantity of constitution chemical substances, low in cost, and convenient to prepare. By using the method disclosed by the invention, potato virus-free seedlings can be propagated in an opening environment, and the characteristics that the propagation speed is high, the production cost is low, the popularization and the application are easy, and the like can be realized.
Owner:GANSU KAIKAI AGRI TECH DEV
Method for synchronously detecting three kinds of viruses on tobacco through triple one-step method RT-PCR (Reverse Transcription Polymerase Chain Reaction)
ActiveCN102242224AReduce cross contaminationReduces the possibility of cross-contaminationMicrobiological testing/measurementMicroorganism based processesNicotiana tabacumTobacco mosaic virus
The invention discloses a method for synchronously detecting three kinds of viruses on tobacco through triple one-step method RT-PCR (Reverse Transcription Polymerase Chain Reaction). The method is characterized by being used for synchronously detecting TMV (Tobacco Mosaic Virus), PVY (Potato Virus Y) and CMV (Cucumber Mosaic Virus), the method comprises the following steps of screening and comparing conservation areas in a virus gene group, artificially screening and synthesizing specificity primers suitable for composite RT-PCR of three kinds of virus diseases; analyzing to obtain TMV, PVY and CMV infection tobacco leaf samples; and integrating enzymes and a buffer solution required by a reverse transcription and a PCR (Polymerase Chain Reaction), and establishing a method for compositely detecting the TMV, PCY and CMV infection of tobacco leaves based on one-step method RT-PCR through optimization of reaction conditions. The triple one-step method RT-PCR utilized by the invention has the advantages of simple and convenient operation, time and labor saving, reliable result and low cost; simultaneously, the probability of cross-contamination of samples in an operation process canbe effectively reduced, and the monitoring on the TMV, PVY and CMV in tobacco agricultural production has wide application prospect.
Owner:ZHENGZHOU TOBACCO RES INST OF CNTC
Method for cultivating tobacco capable of resisting various viruses by adopting RNAi (RNA interference) technique
ActiveCN102943091ATMV resistantGood antiviral effectGenetic engineeringFermentationBiotechnologyNicotiana tabacum
The invention relates to a method for cultivating tobacco capable of resisting various viruses by adopting an RNAi (RNA interference) technique. The method comprises the following steps of: obtaining relatively conservative areas of four kinds of viruses within a genome range through screening by comparing full-length sequences of multiple genomes of four kinds of viruses, i.e. CMV (cucumber mosaic virus), PVY (potato virus Y), PVX (potato virus X) and TMV (tobacco mosaic virus) in a genBank; selecting virus sequences and artificially synthesizing 800bp mosaic genes; and accordingly constructing RNAi plant expression carriers of the mosaic genes and transforming common tobacco through agrobacterium to obtain transgenic plants. The method for cultivating tobacco capable of resisting various viruses by adopting the RNAi technique has the characteristics that the four kinds of major tobacco viruses in China are selected as targets, the artificially constructed hairpin structures comprising the sequences of the four kinds of viruses are transformed into the tobacco by using the plant genetic engineering technique, a hairpin double-strand RNA structure transcribed by the tobacco is cut into siRNA (small interfering RNA) by the plant self mechanism, the normal duplication and the accumulation of target virus genes in tobacco plants are specifically interfered, degraded or silenced, and new tobacco materials capable of resisting various viruses can be obtained through screening.
Owner:ZHENGZHOU TOBACCO RES INST OF CNTC
Method for removing potato virus by virazole
InactiveCN102599057AOptimum use concentrationEffective in removing large quantitiesHorticulture methodsPlant tissue culturePotato virus YSeedling
Owner:HUAZHONG AGRI UNIV
Construction methods and applications of PVX (potato virus X) over-expression vectors and BiFC (bimolecular fluorescence complementation) vector
The invention relates to the field of genetic engineering and provides construction methods and applications of two PVX (potato virus X) over-expression vectors and one BiFC (bimolecular fluorescence complementation) vector. A genome of a PVX 1985 isolate is cloned to a downstream 35S promoter through genetic recombination, obtained infectious clone can infect solanaceae crops such as tobacco, tomatoes, potatoes and the like through agrobacterium infiltration. Infectious clone is reconstructed, and the over-expression vectors capable of effectively expressing one or two types of heterologous protein in a host plant body as well as the BiFC vector capable of identifying interactions between protein are obtained.
Owner:SHANDONG AGRICULTURAL UNIVERSITY
A pvy/cmv surface plasmon resonance detection method
InactiveCN102297967AHigh sensitivityEasy to operatePhase-affecting property measurementsImmunoglobulins against virusesAntigenSurface plasmon
The invention discloses a surface plasma resonance detecting method for PVY / CMV (Potato Virus Y / Cucumber Mosaic Virus) and belongs to a material detection method. The surface plasma resonance detecting method comprises the following steps: 1) preparing a PVY / CMV antigen and a monoclonal body; 2) preparing a biosensor chip; 3) preparing a standard curve and acquiring a virus computational formula; and then measuring the content of PVY / CMV by utilizing the virus computational formula. The surface plasma resonance detecting method provided by the invention has high sensitivity, can be used for qualitatively and quantitatively measuring PVY / CMV and is convenient in operation and low in cost.
Owner:HENAN AGRICULTURAL UNIVERSITY
Plant source antiviral agent containing phyllanthi fructus and preparation method thereof
ActiveCN103766414AHigh activityGood environmental compatibilityBiocideDisinfectantsNicotiana tabacumTobacco mosaic virus
The invention discloses a plant source antiviral agent which takes a phyllanthi fructus L. extractive as an effective component and a preparation method thereof. The preparation method comprises the following steps: crushing phyllanthi fructus L. and carrying out a certain processing process to prepare a phyllanthi fructus L. antiviral soluble liquid agent, an aqueous agent and micro-emulsion. The antiviral agent can be used for preventing and treating tobacco mosaic virus (TMV), cucumber mosaic virus (CMV), potato virus X (PVX), potato virus Y (PVY) and the like on crops including tobaccos, chilies, tomatoes, pumpkins, potatoes and the like. The preparation contains 10%-30% of the phyllanthi fructus L. extractive and a residual amount of an auxiliary agent. The plant antiviral agent not only has a good antiviral effect, but also is safe to environments, human beings, livestock and natural enemies of pests, and other beneficial organisms. The plant antiviral agent is simple in preparation process and low in cost, and is suitable for popularization and application.
Owner:NORTHWEST A & F UNIV
TMV-CMV-PVY three-virus (tobacco mosaic virus, cucumber mosaic virus and potato virus) colloidal gold rapid test strip
ActiveCN106636004ALow costSimple and fast operationImmunoglobulins against virusesTissue cultureBALB/cTobacco mosaic virus
The invention discloses a TMV-CMV-PVY three-virus (tobacco mosaic virus, cucumber mosaic virus and potato virus) colloidal gold rapid test strip, which is composed of a sample pad, a colloidal gold pad, an NC membrane, a piece of water-absorbent filter paper and a backing, wherein a colloidal gold labeled TMV specific antibody, a CMV specific antibody and a PVY specific antibody are enveloped on the colloidal gold pad, and the three antibodies are secreted from hybridoma cell strains BALB / c 15 50, BALBc 15 27 and BALBc 15 8; a detection line and a control line are arranged on the NC membrane; specific antigens of three viruses are enveloped on the detection line; and a colloidal gold labeled second antibody is enveloped on the control line. The test strip is especially suitable for detecting the TMV, CMV and PVY in Guangdong tobacco-growing areas, and the test strip is rapid, sensitive, accurate, low in cost and convenient to operate; the test strip is capable of simultaneously diagnosing various viruses by conducting sampling once; the test strip is quite suitable for in-situ preliminary screening of a great batch of samples; and the test strip, in actual production, has a high application value, and the test strip has a good popularization and application prospect.
Owner:SOUTH CHINA AGRI UNIV +2
Method for compositely detecting tobacco common mosaic virus and potato virus Y in one step and primers thereof
ActiveCN102140546BEasy to detectStrong specificityMicrobiological testing/measurementDNA/RNA fragmentationTobacco mosaic virusViral disease
Owner:ZHENGZHOU TOBACCO RES INST OF CNTC
Rapid test strip for potato viruses, and preparation method and application thereof
The invention discloses a rapid test strip for potato viruses, and a preparation method and application thereof. The rapid test strip for the potato virus comprises a sample pad, a colloidal-gold pad, a NC membrane, a test line (a T line), a control line (a C line), a water absorbing pad and a PVC base plate. The preparation method comprises the following four steps: preparation of the colloidal-gold pad; preparation of the NC membrane; preparation of the sample pad; and assembling of the test strip. Application and detection comprise the following concrete steps: treatment of a sample; dropwise addition of the sample; result interpretation; etc. The potato viruses comprise PVX, PVYNTN, PVYo, PLRV, PVS, PVM, PVA and a tomato black ring virus. The test strip is convenient to use, has good applicability, needs no laboratory, no special apparatus and no operation training of professionals, can realize testing in any place with potatoes, such as a field, a potato storage cellar and a customhouse, and can be used by any potato planter.
Owner:KESHAN BRANCH OF HEILONGJIANG ACADEMY OF AGRI SCI
Cultivation method of virus-free seed tuber of Solanum tuberosum
InactiveCN105409500AImprove qualityImprove germination rateCultivating equipmentsPlant cultivationDiseasePotato leaf roll virus
The invention discloses a cultivation method of a virus-free seed tuber of Solanum tuberosum and relates to the technical field of planting of Solanum tuberosum. The method comprises sowing whole tubers of virus-free seed tubers in a high-altitude aphid-free area with an elevation of 2500-2600 m, wherein each whole tuber weighs 35-45 g, and cultivating the virus-free seed tubers by means such as pre-sewing sufficient application of base fertilizers, germination accelerating and reasonable close planting. The emergence rate is high, up to 99.8%, a course of seedling emergence to maturity takes about 70 days, seedlings grow evenly during growth without disease and pest damage, harvested seed tubers are good in quality, total virus (PVY (Potato Virus Y) and PLRV (Potato Leaf Roll Virus)) rate is 4%, bacterial wilt rate is 0.35%, indexes comply with related specifications in seed tubers of Solanum tuberosum GB 18133-2012, the yield per mu can be increased by 35%, and the yield of small potatoes is up to 70%.
Owner:巫溪县薯光农业科技开发有限公司
Recombinant vector, method for giving immunity against PVY-T to potato plant, and potato plant having immunity against PVY-T
InactiveUS6228637B1Avoid problemsEffective preventionSsRNA viruses positive-senseVirus peptidesPotato virus YPlant cell
A recombinant vector which can transform potato plants so as to confer upon the potato plant immunity against potato virus Y necrosis line is disclosed. The recombinant vector according to the present invention comprises a promoter which functions in a potato plant cell; an operably linked leader sequence of RNA4 of cucumber mosaic virus, which is located downstream of the promoter; and an operably linked sequence which encodes a coat protein of potato virus Y necrosis line, which is located downstream of said leader sequence; and the recombinant vector is capable of transforming potato plants.
Owner:JAPAN TOBACCO INC
Hydroponic bed for potato virus-free seedlings
InactiveCN107637505AOptimal breeding temperatureQuality improvementAgriculture gas emission reductionCultivating equipmentsTemperature controlPotato virus Y
The invention discloses a hydroponic bed for potato virus-free seedlings and relates to the technical field of hydroponics for potato virus-free seedlings. The hydroponic bed comprises a bed body, a fixing plate is arranged in the bed body, and multiple seedling holes used for storing nutrition solutions for seedling are formed in the fixing plate; water serving as an insulation medium is filled between the fixing plate and the bed body, a heating device and a temperature controlling device are further arranged on the bed body, and the heating device and the temperature control device are cooperated with each other to have water temperature regulated; the temperature control device comprises a display and a heat sensor, and the heating device and the heat sensor are immersed in water. By the arrangement, the temperature of the hydroponic bed can be regulated to promote rapid growth of the hydroponic seedlings, time for entering mist culture is shortened, quality of stock seeds of potatoes is improved, and yield is increased; when the seedling viruses occur, infection sources cannot be spread with the nutrition solutions, and the viruses are easily controlled.
Owner:宋宏婷
Multi-PCR (Polymerase Chain Reaction) method for synchronously detecting four chilli viruses
InactiveCN106755597ASolve technical bottlenecksWith characteristicsMicrobiological testing/measurementMicroorganism based processesDiseasePotato virus Y
Owner:INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
Colloidal gold test strip for rapidly detecting double viruses of tobacco mosaic virus-potato virus Y (TMV-PVY)
InactiveCN106244561ALow costSimple and fast operationMicroorganism based processesImmunoglobulins against virusesBALB/cTobacco mosaic virus
The invention discloses a colloidal gold test strip for rapidly detecting double viruses of tobacco mosaic virus-potato virus Y (TMV-PVY). The test strip consists of a sample pad, a colloidal gold pad, an NC film, a piece of water absorption filter paper and a back lining, wherein the colloidal gold pad is enveloped with a TMV specific antibody and a PVY specific antibody which are marked by colloidal gold, and the TMV specific antibody and the PVY specific antibody are respectively generated by secretion by hybridoma cell lines, i.e., BALB / c-15-50 and BALB / c-15-8; the NC film is provided with a detection line and a control line; the detection line is enveloped with specific antigens of the TMV and the PVY; the control line is enveloped with a second antibody marked by the colloidal gold. The rapid detection colloidal gold test strip is especially suitable for the detection on the TMV and the PVY in Guangdong tobacco-growing areas, and is rapid, sensitive and accurate in dection, low in cost and simple and convenient in operation; the test strip can be used to diagnose the two viruses at the same time by one-time sampling, thus being very suitable for field primary screening of a great batch of samples; therefore, the colloidal gold test strip has a good application value in actual production and is good in popularization and application prospects.
Owner:SOUTH CHINA AGRI UNIV
Planting method and planting structure capable of reducing flue-cured tobacco aphid-spread disease
InactiveCN102318491AImprove yield and qualityHigh activityHorticultureBiotechnologyNicotiana tabacum
The invention discloses a planting method and a planting structure capable of reducing flue-cured tobacco aphid-spread diseases. The method is to plant Triticeae crops between every two rows of flue-cured tobaccos. By planting the Triticeae crops between every two rows of flue-cured tobaccos, the Triticeae crops can be used as a natural barrier for flue-cured tobacco transplanting seedlings to enable migratory tobacco aphids to firstly stop on the Triticeae crops to suck juice, the viruses borne by the aphids can be released, the direct harm of the tobacco aphids can be greatly reduced, the probability of spreading tobacco viruses can be decreased, the aphid-spread diseases such as tobacco potato virus Y diseases and the like are reduced and the product quality of the flue-cured tobaccos is stabilized and improved. According to the investigation and the statistics of the applicant, by interplanting the Triticeae crops and the flue-cured tobaccos, the incidence rate of tobacco virus diseases spread by the tobacco aphids is below 50 percent.
Owner:ZUNYI TOBACCO OF GUIZHOU TOBACCO CORP
Soil cultivation method for potato virus-free basic seeds
ActiveCN105145042ALow pollution rateReduce the cost of trainingAgriculture gas emission reductionCultivating equipmentsNutrient solutionBottle
The invention discloses a soil cultivation method for potato virus-free basic seeds. The method comprises: 1) production of a tissue culture seedling; 2) rooting and strengthening; 3) soil cultivation; and 4) harvesting and storage, wherein the step 2) rooting and strengthening comprise: taking out the tissue culture seedling suitable for fixed planting out of a culture bottle; removing root systems and cleaning a culture medium; soaking the roots for 10-15 minutes by using a NAA50 mg / L+2-4 D 5 mg / L rooting agent; then fixedly planting the roots in a soilless culture medium, and keeping the temperature and humidity by using a plastic arch-shaped shed to accelerate the tissue culture seedling to take root, sprout and quickly grow; 12 days after fixed planting, starting spraying a nutritional liquid, spraying the nutritional liquid once at an interval of five days, and spraying the nutritional liquid three times; in 29-32 days after fixed planting, uncovering plastic of the arch-shaped shed, and selecting robust tissue culture seedlings, wherein the height of the seedlings reaches 10 cm and the blades are longer than 1 cm, and transplanting the tissue culture seedlings in pre-treated field soil. By adopting the soil which replaces the medium to culture the potato virus-free basic seeds, the production cost is greatly lowered, so that the market supply price is lowered, and the method plays a positive role in generalizing and popularizing virus-free potatoes.
Owner:定西金麟种业有限责任公司
Method for cultivating potato virus-free seedlings
InactiveCN103250640APromote formationGuaranteed to surviveHorticulture methodsPlant tissue cultureContamination rateNutrient solution
The invention discloses a method for cultivating potato virus-free seedlings, relates to the virus elimination and tissue culture techniques of plants, and belongs to the field of agricultural seedling breeding. The method is characterized by comprising the following steps of: transferring virus-free potato tissue culture seedlings into a MS culture medium to culture, wherein a bacteriostatic agent and sugar are added into the MS culture medium; and after the culturing is completed, soaking the seedlings into a CIP improved rooting agent, and carrying out cuttage planting and nutrient solution applying on the seedlings, and after the seedlings are mature, harvesting the seedlings. The method disclosed by the invention has the beneficial effects that through cultivating virus-free seedlings in a special culture medium, the contamination rate in the culture medium is reduced; then, the plant seedlings cultivated in the culture medium are subjected to cuttage, and before cuttage, the cuttage seedlings are placed in the CIP improved rooting agent, and nutrient solutions N, P and K are applied, so that the survival of virus-free seedlings is ensured, the potato growth amount and the expansion of tubers are promoted, thereby improving the yield; and the single-plant potato growth amount of breeder seeds is improved, so that the production cost of breeder seeds is reduced by 25%, thereby providing a reliable technical support for the industrialized development of potatoes.
Owner:定西市旱作农业科研推广中心
Overlapped type culture disc for potato virus-free seedlings and application method therefor
ActiveCN104982321AAvoid harmReduce loss rateAgriculture gas emission reductionCultivating equipmentsPotato virus YEngineering
The present invention provides an overlapped type culture disc for potato virus-free seedlings and an application method therefor. The overlapped type culture disc comprises overlapped type culture disc bodies (1), planting holes (2), separation slide blocks (3), a thread hole (4), a separation slide block gap (5), a separation slide block rail (6), a separation handle (7), a bolt fixing hole (8), a blade rail fixing groove (9), a fixed bolt (10), a blade rail (11), a blade frame (12) and a push rod (13). The method has good controllability and strong operability; matched equipment has a simple structure and is efficient and practical; manpower is saved; and the production cost of the potato virus-free seedlings is remarkably reduced by the method and the production efficiency is improved.
Owner:ENSHI JUXIN MODERN AGRI DEV
Method for detecting potato virus Y by using digital PCR and special complete set of reagents thereof
InactiveCN106868208AStrong specificityHigh sensitivityMicrobiological testing/measurementDNA/RNA fragmentationDiseaseAgricultural science
The invention discloses a method for detecting potato virus Y by using digital PCR and a special complete set of reagents thereof. The complete set of reagents is prepared from a primer pair X1 and a probe PVY-p, wherein the primer X1 consists of a primer PVY-f and a primer PVY-r; the primer PVY-f is a single-chain DNA molecule expressed by a sequence 1 in a sequence table; the primer PVY-r is a single-chain DNA molecule expressed by a sequence 2 in the sequence table; the sequence of the probe PVY-p is a sequence 3 in the sequence table; an FAM fluorescent mark is arranged at a 5'end of the probe PVY-p; a TAMRA fluorescent mark is arranged at a 3'end of the probe PVY-p. Experiments show that the method for detecting the potato virus Y by using the digital PCR is high in specificity and high in sensitivity, is applicable to detection of latent diseases and the PVY in samples with low virus content, and has an important application value.
Owner:CHINESE ACAD OF INSPECTION & QUARANTINE
Method for rapidly removing potato virus
ActiveCN106718887AReduce the rate of poisoningInhibition of replicationPlant growth regulatorsBiocidePotato virus YInducer
The invention discloses a method for rapidly removing a potato virus. The method comprises the steps that a plant resistance inducer for inhibiting the virus is sprayed on a tissue culture seedling at 3-5 days before stem tip peeling based on conventional stem tip detoxication, wherein the plant resistance inducer includes but is not limited to amino-oligosaccharin, ningnanmycin, 3-hydroxyl-3-acetonyl ketoindole, and the like; a stem tip is taken and separately cultured for 7-28 days; the plant resistance inducer for inhibiting the virus is sprayed for one time; 0.1-0.7mm stem tip is taken and separately cultured for 7-28 days; and the step is repeated for three times to obtain a virus-free tissue culture seedling. A virus-free rate of the obtained tissue culture seedling is higher than 80%. According to the method, the time for obtaining the virus-free tissue culture seedling is short; a virus rate of the obtained tissue culture seedling is low; operation is simple; and production popularization is facilitated.
Owner:云南省农业科学院生物技术与种质资源研究所
Method for purification and rejuvenation of potato virus-free tissue culture seedlings
ActiveCN103518624APromote growthFast growthPlant tissue cultureHorticulture methodsPotato virus XPotato virus Y
The invention discloses a method for purification and rejuvenation of potato virus-free tissue culture seedlings. The method is characterized in that a culture medium suitable for growth of potato tissue culture seedlings is prepared through an MS culture medium, composite carrageenan and alpha-pimacol, the pre-cultured virus-free tissue culture seedlings are placed in the solid culture medium and cultured for 12 days, roots and stems of the virus-free potato tissue culture seedlings are strong, and the survival rate of seedling acclimatization and transplantation is high. Compared with an existing potato tissue culture seedling medium, the culture medium has the advantages of being low in cost and good in economic benefit.
Owner:GANSU KAIKAI AGRI TECH DEV
RT-PCR detection kit for GMBFV, PVY and GCLV viruses of garlic
InactiveCN112779360AStrong specificityEfficient distribution and utilizationMicrobiological testing/measurementMicroorganism based processesGarlic mite-borne filamentous virusPotato virus Y
The invention discloses a RT-PCR detection kit for GMBFV, PVY and GCLV viruses of garlic, which is characterized in that RNA of garlic tissue is used as a template, cDNA is obtained through reverse transcription, then three pairs of specific primers are adopted, one-time RT-PCR is carried out to detect the main garlic viruses simultaneously, such as the Garlic mite borne filamentous virus, the Potato virus Y and the Garlic common latent virus, and the three pairs of primers are shown in SEQ ID NO.1-SEQ ID NO.6. The detection kit provided by the invention has high detection efficiency, low detection cost and is suitable for virus detection of large-scale garlic materials.
Owner:INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
Detection method for poison of CMV (Cytomegalovirus) and PVY (Potato Virus Y) of single-head tobacco aphids
InactiveCN103645309AInstant detectionStrong specificityBiological material analysisCelluloseVirus Protein
Owner:GUIZHOU UNIV
Tobacco eIFiso4E-T mutant and application thereof in cultivation of tobacco resisting potato virus Y
The invention discloses a tobacco eIFiso4E-T mutant and an application thereof in cultivation of tobacco resisting potato virus Y. The amino acid sequence of the tobacco eIFiso4E-T mutant is as shownin SEQ ID NO: 2 or SEQ ID NO: 3. Compared with an amino acid sequence of wild tobacco eIFiso4E-T shown as SEQ ID NO: 1, the 76th site of the amino acid sequence of the tobacco eIFiso4E-T mutant is mutated from A to T or the 98th site is mutated from G to D. The eIFiso4E-T mutant is polymerized with a va site resisting the potato Y virus or an eifiso4e-tKO gene, so that the lasting resistance of the tobacco to the PVY can be obtained. The eIFiso4E-T mutant disclosed by the invention has an important significance in cultivating tobacco with lasting resistance to the PVY.
Owner:YUNNAN ACAD OF TOBACCO AGRI SCI
Screening of potato virus X (PVX) low virulent strains and application in cross protection
ActiveCN103820400AReduce pathogenicityFree from infectionInactivation/attenuationMicroorganism based processesGeneticsPotato virus Y
The invention relates to the field of plant virus gene engineering, and provides screening of potato virus X (PVX) low virulent strains and application in cross protection. Based on infectious clones of PVX strong virulent strains, mutants are introduced into PVX genomes in a directed site manner through a site-directed mutagenesis technology, so as to obtain PVX low virulent strains E46A, N863A, N968A and E1001A with significantly reduced pathogenicity. The low virulent strain E1001A can effectively protect plants from being infected by the PVX strong virulent strains.
Owner:SHANDONG AGRICULTURAL UNIVERSITY
Use of tobacco gene nttctp in plant against potato virus y
InactiveUS20180346922A1Improve the immunityPlant peptidesVector-based foreign material introductionBiotechnologyNicotiana tabacum
Provided is a use of tobacco gene NtTCTP for resisting potato virus Y in plants (PVY). The tobacco gene has a full length of 507 bp and encodes a protein containing 168 amino acids, the activity of which is closely related to the PVY-resistance of tobacco. When the gene is silenced in tobacco, tobacco shows stronger resistance to PVY and can reach immune levels; and when the gene is overexpressed in the tobacco; and tobacco becomes more sensitive to PVY. The gene can be used in the breeding of PVY-resistant plants such as tobacco.
Owner:GUIZHOU TOBACCO SCI RES INST
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