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Potato virus-free seedling tissue culture propagation method

A technology for potato and virus-free seedlings, which is applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problem of high cost of nutrient solution, and achieve the effects of less chemical substances, convenient preparation and low production cost

Inactive Publication Date: 2014-01-29
GANSU KAIKAI AGRI TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The object of the present invention is to provide a method for tissue culture and propagation of virus-free potato seedlings, to solve the problem of relatively high cost of nutrient solution for liquid culture of potato-free seedlings at present, and to provide a nutrient solution for liquid culture of virus-free potato seedlings with low cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] 1. Selection treatment: choose seed potatoes as explants, take the seed potatoes out of the cellar 20 days before sowing, select, and select young and mature tubers with obvious variety characteristics, regular potato shape, smooth skin, and consistent bud eye depth. Potatoes with irregular shape, rough and aging skin, protruding bud eyes, cracked heads, dull skin color and other unhealthy potato cubes should be eliminated;

[0017] 2. Pregermination and sun-drying: before sowing, the potato seed potatoes are inserted into the germination medium for cultivation and growth; the medium formula used is: KNO 3 2000mg / L, KH 2 PO 4 400mg / L, (NH 4 ) 2SO4400mg / L, CaCl 2 400mg / L, EDTA-Na 2 50mg / L, FeSO 4 50mg / L, B940mg / L, NAA0.5mg / L, 6-BA0.5mg / L, coconut milk 100ml / L, mannitol 1g / L, activity 0.5g / L, sucrose 25g / L, agar 3g / L nutrition The solution is prepared with sterile water; the planted trees are selected and placed at 15°C to accelerate germination. When most of the bu...

Embodiment 2

[0021] 1. Selection treatment: choose seed potatoes as explants, take the seed potatoes out of the cellar 20 days before sowing, select, and select young and mature tubers with obvious variety characteristics, regular potato shape, smooth skin, and consistent bud eye depth. Potatoes with irregular shape, rough and aging skin, protruding bud eyes, cracked heads, dull skin color and other unhealthy potato cubes should be eliminated;

[0022] 2. Pregermination and sun-drying: before sowing, the potato seed potatoes are inserted into the germination medium for cultivation and growth; the medium formula used is: KNO 3 2200mg / L, KH 2 PO 4 550mg / L, (NH 4 ) 2SO 4 500mg / L, CaCl 2 500mg / L, EDTA-Na 2 60mg / L, FeSO 4 60mg / L, B960mg / L, NAA1.0mg / L, 6-BA1.0mg / L, coconut milk 100ml / L, mannitol 1g / L, activity 0.5g / L, sucrose 25g / L, agar 3g / L nutrition The solution is prepared with sterile water, the EC of the nutrient solution is 0.5-0.9, and the pH value is 5.8-6.2; the planted trees ar...

Embodiment 3

[0026] 1. Selection treatment: choose seed potatoes as explants, take the seed potatoes out of the cellar 20 days before sowing, select, and select young and mature tubers with obvious variety characteristics, regular potato shape, smooth skin, and consistent bud eye depth. Potatoes with irregular shape, rough and aging skin, protruding bud eyes, cracked heads, dull skin color and other unhealthy potato cubes should be eliminated;

[0027] 2. Pregermination and sun-drying: before sowing, the potato seed potatoes are inserted into the germination medium for cultivation and growth; the medium formula used is: KNO 3 2100mg / L, KH 2 PO 4 500mg / L, (NH 4 ) 2SO 4 450mg / L, CaCl 2 450mg / L, EDTA-Na 2 55mg / L, FeSO 4 55mg / L, B950mg / L, NAA0.7mg / L, 6-BA0.7mg / L, coconut milk 100ml / L, mannitol 1g / L, activity 0.5g / L, sucrose 25g / L, agar 3g / L nutrition The solution is prepared with sterile water, the EC of the nutrient solution is 0.5-0.9, and the pH value is 5.8-6.2; the planted trees ar...

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Abstract

The invention discloses a potato virus-free seedling tissue culture propagation method which relates to the propagation technology of potatoes and belongs to the technical filed of agriculture planting. The method is characterized by comprising the following steps: performing fine selection, performing accelerating germination, performing seed airing, and inoculating aired seeds in a germination culture medium for cultivation and growth, wherein the accelerating germination and sowing are performed at the temperature being 15-20 DEG C; the method has the beneficial effects that compared with a formula based on an MS, a formula in the method is small in quantity of constitution chemical substances, low in cost, and convenient to prepare. By using the method disclosed by the invention, potato virus-free seedlings can be propagated in an opening environment, and the characteristics that the propagation speed is high, the production cost is low, the popularization and the application are easy, and the like can be realized.

Description

technical field [0001] The invention relates to seed potato propagation technology, which belongs to the field of agricultural planting technology, in particular to a method for tissue culture propagation of virus-free potato seedlings. Background technique [0002] Potatoes are one of the traditional dominant crops in Dingxi. With the steady development of the potato industry, the planting area and benefits have increased year by year, and it has become one of the main potato industry bases in the province. The city's potato sowing area reached 18,000 hectares, accounting for 25% of the total crop sowing area, with an average fresh potato production of 35,250 kg / ha, and a total output of 634.5 million kg. After several years of construction and development, the city's potato planting has formed a large-scale and intensive pattern, forming a seed potato breeding area dominated by the alpine and damp areas of Guanshan, a late-maturing high-starch commercial potato production ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 李恺屠建章张才强火有仁吴成秀
Owner GANSU KAIKAI AGRI TECH DEV
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