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89results about How to "Instant detection" patented technology
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Adaptively adjusted laser projection module and depth camera
InactiveCN107167996AReduce volumeInstant detectionProjectorsCamera body detailsLight beamOptoelectronics
The invention provides an adaptively adjusted laser projection module. The module comprises a light source, a specific optical element, a pattern generator and a light beam detector. The specific optical element comprises at least one light dividing face, wherein each light dividing face is used for reflecting and transmitting at least parts of light beams emitted by the light source. The pattern generator receives light beams parts of which are reflected or transmitted through the light dividing face, and externally emitting structure light patterns. The light beam detector is used for receiving the light beams parts of which are reflected or transmitted through the light dividing face, and detecting the intensity of the light beams. The invention also provides a depth camera. The depth camera comprises the adaptively adjusted laser projection module and is used for projecting structuralized light beam images towards a target space. According to the invention, by use of the specific optical element having the reflection and transmission functions, the laser projection module has quite small size; and by combining an optical detector, the light source can be detected anytime and anywhere, so laser intensity can be further controlled and damage to human is avoided.
Owner:SHENZHEN ORBBEC CO LTD
Detection apparatus and detection method
InactiveCN101369059AFast detection methodImprove product yieldOptical apparatus testingNon-linear opticsOptical pathPolarizer
The invention relates to a detecting device, which is suitable for detecting the angle of a light absorption axis of a first polarizer of a liquid crystal panel, and the liquid crystal panel comprises a first baseplate, a second baseplate and a liquid layer. The detecting device includes a light source, a rotatable detecting polarizer and a light detector. The light source is arranged at one side of the liquid crystal panel, and the liquid crystal panel is arranged on the optical path of the light source. The rotatable detecting polarizer is arranged at the other side of the liquid crystal panel and is arranged on the optical path of the light source, and the first polarizer is arranged between the baseplate and the rotatable detecting polarizer. The optical detector is arranged on the optical path of the light source, and the optical detector and the first polarizer are respectively arranged at two sides of the rotatable detecting polarizer; so using the detecting device can rapidly detect the angle of the light absorption axis of the first polarizer.
Owner:CHUNGHWA PICTURE TUBES LTD
Sample pretreatment method for detection of organochlorine pesticide residue in food
The invention discloses a sample pretreatment method for detection of organochlorine pesticide residue in food and is characterized by comprising the following steps: water is added to a food sample, sample solution is swirled, and then inorganic salt and hydrophilic organic solvent are added to the sample solution, the sample solution is swirled and shaken uniformly, the sample solution undergoes phase splitting after centrifugation at room temperature, the upper-phase liquid is purified through dispersion solid-phase extraction method, the extraction temperature is 20-30 DEG C, and finally the upper-phase liquid is detected by gas chromatography; and the mass percent of the inorganic salt accounts for 5%-35% of the total mass of the system, the mass percent of the hydrophilic organic solvent accounts for 10%-50% of the total mass of the system, and water is of the residual amount, and the total mass of the system is the mass sum of water contained in the sample, the added water, the inorganic salt and the hydrophilic organic solvent. According to the sample pretreatment method, a dual-water-phase system consisting of the hydrophilic organic solvent, the organic salt and water is utilized, the partition coefficient is increased by means of changing the types and concentrations of the organic solvent and the inorganic salt, and the organochlorine pesticide residue in the sample can be directly extracted at room temperature.
Owner:YANTAI UNIV
Colloidal gold detection card for rapidly detecting melamine and preparing method thereof
InactiveCN101413956AChange limitationsImprove detection accuracyMaterial analysisNitrocelluloseMonoclonal antibody
The invention provides a colloidal gold cassette for rapid detection of melamine, the colloidal gold cassette has rapid detection, convenient carry and simple operation, the invention also provides a preparation method of the cassette, the preparation process thereof is simple, and the cost is low. The colloidal gold cassette comprises a test strip, the test strip is packaged in a shell body of the cassette, a sample adding hole and an observation hole are arranged on the shell body of the cassette, the test strip comprises a bottom plate, the colloidal gold cassette is characterized in that water absorbent paper, a nitrocellulose membrane, a gold-labeled pad and a sample pad are sequentially arranged on the bottom plate of the test strip, the water absorbent paper and the gold-labeled pad are respectively arranged at the two ends of the nitrocellulose membrane, small sections of the water absorbent paper and the gold-labeled pad are respectively overlapped on the nitrocellulose membrane at the junctions at the two ends of the nitrocellulose membrane, the sample pad is arranged at the other end of the gold-labeled pad, a small section of the sample pad is overlapped on the gold-labeled pad; a gold-labeled anti-melamine monoclonal antibody is sprayed on the gold-labeled pad; a detection line with the material of a melamine-ovalbumin coupled conjugate and a quality control line with the material of goat anti-mouse IgG are sequentially sprayed on the nitrocellulose membrane.
Owner:江苏省苏微微生物研究有限公司
Electrochemical biosensor based on aptamer/nano-silver probes and EXO I enzyme
ActiveCN106198673ASensitive and efficient detectionSimple and fast operationMicrobiological testing/measurementMaterial electrochemical variablesAptamerElectrochemical biosensor
Owner:QINGDAO UNIV
hCG cycle detection test paper, hCG cycle detection kit, and preparation method and applications of hCG cycle detection test paper
InactiveCN108761099AEasy to operateThe detection method is simpleBiological material analysisBiological testingAnimal scienceAntiendomysial antibodies
Owner:NANTONG EGENS BIOTECH CO LTD
Ballastless track bed plate elevation rapid inspection vehicle
InactiveCN103255689AInstant detectionImprove construction efficiencyMeasuring apparatusPhysicsVehicle frame
The invention discloses a ballastless track bed plate elevation rapid inspection vehicle which comprises fixing wheels which are transversely stretched on two sides of a bed plate. A vehicle frame is arranged on the fixing wheels. A prism, a tilt angle sensor and four sonar sensors are arranged on the vehicle frame. The prism, the tilt angle sensor and the four sonar sensors are connected with a total station. The aim that an elevation error of the upper surface of the bed plate is rapidly measured is achieved. The ballastless track bed plate elevation rapid inspection vehicle has the functions of field measurement, calculation, display and storage and can rapidly carry out detection and evaluation in real time. A special quality control device and method is provided for practical construction of a ballastless track. Construction efficiency is improved. Construction cost is reduced. A construction technical level of the ballastless track is improved.
Owner:THE FIFTH ENG CO LTD OF CHINA TIESIJU CIVIL ENG GRP
Dialysis device and silicon nanowire field-effect tube combined biosensor
The invention relates to a dialysis device and silicon nanowire field-effect tube combined biosensor. The dialysis device and silicon nanowire field-effect tube combined biosensor comprises a dialysisdevice, a silicon nanowire field-effect tube and a signal output computer, wherein the dialysis device is communicated with the silicon nanowire field-effect tube through a thin silica gel flexible pipe; and the silicon nanowire field-effect tube transmits a signal to the signal output computer through a probe. The serum desalting dialysis device and the silicon nanowire field-effect tube are connected into the same pipeline; the dialysis device is used as a filter; the field-effect tube having a double gate structure with a semiconductor silicon nanowire as a channel is used as a signal converter; an antibody corresponding to a tumor marker is used as a biological sensitive element; and therefore, the immediate, label-free and high-sensitivity detection of the serum tumor marker is realized.
Owner:WUXI PEOPLES HOSPITAL
Pseudomonas putida test strip and preparation method thereof
ActiveCN104374914AQuick checkMeet the needs of on-site testingMaterial analysisYolkPseudomonas putida
The invention discloses a pseudomonas putida test strip and a preparation method thereof. The pseudomonas putida test strip comprises a sample pad, a jointing pad, a nitrocellulose membrane, a water absorbing pad and a PVC substrate; the pseudomonas putida test strip is characterized in that the sample pad, the jointing pad, the nitrocellulose membrane and the water absorbing pad are orderly bonded to the PVC substrate; the jointing pad is coated with an anti-pseudomonas putida yolk antibody-colloidal gold label; the nitrocellulose membrane is coated with a detection line formed by the ultrasonic broken liquid of the pseudomonas putida and a quality control line formed by rabbit anti-chicken yolk antibodies; the pseudomonas putida test strip has the advantages of high sensitivity, strong specificity, simplicity and convenience in operation, fast detection and high accuracy.
Owner:NINGBO UNIV
Paper chip aptamer sensor and preparation method thereof
InactiveCN109781811AReduce manufacturing costImprove stabilityMaterial electrochemical variablesBiochemical engineeringReaction zone
The invention relates to a paper chip aptamer sensor and a preparation method thereof. The paper chip aptamer sensor comprises four layers of filter paper, microfluidic channels (1), filter membranes(2), reaction zones (3), counter electrodes (4), reference electrodes (5) and working electrode (6); the four layers of filter paper are a first layer of filter paper, a second layer of filter paper,a third layer of filter paper and a fourth a layer of filter paper which are distributed sequentially from top to bottom; the microfluidic channels (1) are disposed on the first layer of filter paperand the third layer of filter paper; the filter membranes (2) are disposed on the first layer of filter paper, the second layer of filter paper, and the third layer of filter paper, and are communicated with the microfluidic channels (1); the reaction zones (3) are arranged on the third layer of filter paper and are communicated with the microfluidic channels (1) on the third layer of filter paper; the counter electrodes (4) and the reference electrodes (5) are disposed on the second layer of filter paper and are located above the reaction zones (3); the working electrodes (6) are disposed onthe fourth layer of filter paper and are located below the reaction zones (3); the surfaces of the working electrodes (6) are modified with a nanocomposite and are fixedly provided with aptamers.
Owner:INST OF ELECTRONICS CHINESE ACAD OF SCI
Self-adjusting system and method for PM 2.5 flue gas flow fields
ActiveCN105135897AInstant control opening and closingInstant control steeringAerodynamic testingControl devices for furnacesControl flowAutomatic control
The invention relates to a self-adjusting system and method for PM 2.5 flue gas flow fields. The self-adjusting system is characterized by comprising flue gas detection devices, an online monitoring device and an automatic-control adjusting device; the flue gas detection devices are distributed at all detection points of a flue gas pipeline and a dust removal box and fixed to the inner wall of the flue gas pipeline and the inner wall of the dust removal box through supporting legs, detection heads of the flue gas detection devices are exposed to the flue gas pipeline by different heights for sampling, and the flue gas detection devices are connected with the online monitoring device and transmit detection and analysis results to a center processing unit of the online monitoring device; the center processing unit controls flow guide plates in the flue gas pipeline to turn and an air pressure pump in the dust removal box to be turned on or off through a real-time synchronous control system of the automatic-control adjusting device according to the distribution condition of the flue gas flow fields. The self-adjusting system and method can detect PM2.5 in a distributed mode or on line, and the flow fields in the flue gas pipeline and the dust removal box can be controlled intelligently.
Owner:HUNAN CHANGZHONG MACHINERY
Device and method capable of detecting stripes of optical glass prefabricated member
ActiveCN104977300AImprove detection accuracyThe device is compactMaterial analysis by optical meansCamera lensOptical path
The invention provides a stripe detection device and a stripe detection method capable of rapidly detecting the stripes of ellipsoidal aspheric pre-compressed prefabricated member. The device for detecting the stripes of the optical glass prefabricated member comprises a laser light source, a beam expanding system and a CCD image processing system in sequence according to a light path, wherein the CCD image processing system comprises a lens and a computer. According to the device, the strips at the inner part of the ellipsoidal aspheric pre-compressed prefabricated member are detected immediately through a stripe interference principle, and the relatively high detection precision can be achieved; the device structure is compact, the occupying space is small, the degree of automation is high, the resolution ratio effect is good, and the purpose of immediately rapidly detecting the stripes of the ellipsoidal aspheric pre-compressed prefabricated member can be achieved. According to the method, the glass does not need to be annealed and subjected to cold processing, a product can be detected immediately, the result can be obtained, site production process and parameters can be regulated timely, and the method can be implemented in an indoor limited space, the operation is convenient, and the detection efficiency is high.
Owner:CDGM GLASS LLC
Redox hydrosol, and making method and application of integrated microbial electrode used for rapid detection of biotoxicity of water
ActiveCN104007154AEasy to prepareGood biocompatibilityMaterial analysis by electric/magnetic meansHYDROSOLElectrochemistry
The invention relates to a redox hydrosol. The redox hydrosol is prepared through the steps of preparing an inorganic-organic composite sol by using a polymer sol and an aminosilane coupling agent, and mixing the composite sol with an electronic mediator. The invention also relates to a making method of an integrated microbial electrode used for the rapid detection of the biotoxicity of water, and an application of the integrated microbial electrode. Microbes and the electronic mediator are fixed on an electrode through the prepared redox hydrosol to make the integrated microbial electrode, and the integrated microbial electrode can be applied in the rapid detection and instant monitoring of the biotoxicity of water. An electrochemical sensor can system constructed by using the integrated microbial electrode made in the invention can instantly monitor the water organism toxicity change and rapidly detect the water organism toxicity value, can realize instant, continuous and rapid detection, and has the characteristics of high analysis sensitivity, low cost, simple operation, portability and the like.
Owner:TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
Method for detecting lens module performance
This invention relates to a sort of method which is used for checking the capability of the lens module, and it includes these approaches in that: offer a future measuring lens module and a checking camera, and this checking lens module consists of the drawtube, the lens which is fixed in the drawtube, and the aperture, and this aperture offers a entrance pupil and a emergent pupil to this future measuring lens module. Shoot this drawtube of this future measuring lens module and the emergent pupil by this checking camera. Check that if the superposition degree of this picture center of this drawtube and the picture center of this emergent pupil is in the equal range, if yes, shoot the drawtube of this future measuring lens module by this checking camera and the entrance pupil again, and then check that if the superposition degree of this picture center of this drawtube and the picture center of this entrancet pupil is in the equal range.
Owner:HONG FU JIN PRECISION IND (SHENZHEN) CO LTD +1
Vibrio qinghaiensis Q67B and separation, screening and application thereof
InactiveCN103045524AImprove temperature adaptabilityStrong salt toleranceBacteriaMicrobiological testing/measurementSewageWater sample
The invention relates to a vibrio qinghaiensis Q67B (CCTCC (China Center for Type Culture Collection) NO: M2010104) and separation, screening and an application of vibrio qinghaiensis Q67B, and belongs to the technical field of the separation, the screening and the application of luminous bacteria of a microbiology. Vibrio qinghaiensis Q67B is a freshwater luminous bacterium by separating and purifying luminous bacteria collected on the body surface of Qinghai kaluga produced from Qinghai Lake, Qinghai Province, and screening through temperature adaptability, then screening through pH value adaptability, and finally screening through poison reaction sensitivity. Vibrio qinghaiensis Q67B is constantly luminous in a clean water body, when the bacterium contacts poison, the luminous intensity is obviously reduced, so the toxicity and concentration of the poison can be judged. When vibrio qinghaiensis Q67B is used for poison detection of surface water such as inland lakes and rivers, groundwater and sewage effluent, the poison detection can be carried out at 10-37 DEG C without adjusting an osmotic pressure and a pH value of water sample, and the poison of the water sample can be distinguished in 10-15min, so that vibrio qinghaiensis Q67B has the advantages of speediness, convenience and sensitivity. Ocean luminous bacteria do not have the above advantages.
Owner:LIHE TECH (HUNAN) CO LTD
Method for rapidly detecting urogenital tract mycoplasmas based on RPA
InactiveCN111763752AQuick checkSensitive detectionMicrobiological testing/measurementMicroorganism based processesMedicineMicrobiology
The invention discloses a method for rapidly detecting urogenital tract mycoplasmas based on RPA in the technical field of mycoplasma. The method for rapidly detecting urogenital tract mycoplasmas based on RPA comprises the following steps: S1, designing and screening primers and probes: according to a 16SrRNA gene and a gap gene of MH, strictly designing five pairs of primers by using an oligo 5software according to an amplification kit analysis and design manual and performing bLAST verification. The kit is used for screening the optimal RPA primer. According to the present invention, the same MH positive specimen is subjected to RPA amplification with the five pairs of primers respectively, and each 50 [mu]l of a reaction system contains 29.5 [mu]l of a primer-free rehydration buffer solution. The method can detect mycoplasmas rapidly, sensitively and immediately, and can effectively perform mycoplasma screening on women and males of the right age.
Owner:台州市中心医院
Test strip for detecting citrobacter freundii and preparation method thereof
The invention discloses a test strip for detecting citrobacter freundii and a preparation method thereof. The test strip comprises a sample pad, a combining pad, a nitrocellulose membrane, a water-absorbing pad and a PVC (Polyvinyl Chloride) bottom board. The test strip is characterized in that the sample pad, the combining pad, the nitrocellulose membrane and the water-absorbing pad are adhered in order and sequence on the PVC bottom board; the combining pad is coated with an anti-citrobacter-freundii egg-yolk antibody-colloidal gold marker; and the nitrocellulose membrane is coated with a detection line formed by citrobacter-freundii ultrasonic breaking liquid and a quality control line formed by rabbit-anti-chicken egg-yolk antibodies. The test strip disclosed by the invention has the advantages of high sensitivity, strong specificity, simplicity in operation, fast detection and high accuracy.
Owner:NINGBO UNIV
Device and method for measuring and calibrating sound intensity
ActiveCN105444874ACalibration signal stableEasy to carrySubsonic/sonic/ultrasonic wave measurementLoudspeakerMicrophone
The invention discloses a device and a method for measuring and calibrating the sound intensity. The device for measuring and calibrating the sound intensity comprises a shell upper cover, a shell, a sleeve, a circuit board, a loudspeaker, a coupling cavity, a coupling element, a coupling cavity bottom cover, and a reference microphone. The device for measuring and calibrating the sound intensity acquires a calibration signal generated by an external device and can be applied in various types of sound intensity measuring and calibrating environments and methods. Meanwhile, through the conduction of the sleeve and the conduction of the shell, a stable and effective sound source is obtained in the coupling cavity. Meanwhile, based on the coupling element of a symmetrical structure, a reference signal and a measurement signal, that are completely consistent with each other, can be obtained. Through comparing a reference signal fed back from the reference microphone and the above signal of the circuit, the calibration signal is more stable and more accurate. At the same time, the device for measuring and calibrating the sound intensity is highly integrated, convenient to carry about and immediate in detection.
Owner:ZHUZHOU TIMES NEW MATERIALS TECH
Magnetic-nanoparticle-modification-based enzyme sensor used for detecting okadaic acid and preparation method of sensor
InactiveCN104977339AHigh load rateImprove detection sensitivityMaterial electrochemical variablesMagnetite NanoparticlesChemistry
The invention relates to a magnetic-nanoparticle-modification-based enzyme sensor used for detecting okadaic acid. The enzyme sensor comprises an insulated base body, and an electrode system including a work electrode, a reference electrode and a counter electrode, which is formed on the base body, wherein a biological enzyme layer is fixed on the work electrode, and comprises magnetic nanoparticle modified protein phosphatase 2A (PP2A) and an electronic transfer body, the diameters of magnetic nanoparticles are 250nm-350nm, and the invention also relates to a preparation method of the enzyme sensor. Compared with a common enzyme sensor, the enzyme sensor has the advantages that the loading rate of PP2A on the surface of the work electrode is improved through the magnetic nanoparticle modified PP2A, so that the current response value of the enzyme sensor is improved by 10 times or more compared with the common PP2A sensor, and when the enzyme sensor is used for detecting okadaic acid of an aquatic product, the detection limit is lower than 0.20microgram / L, the reproducibility RSD is smaller than 5%, and the detection time consumption is smaller than 0.5h.
Owner:ZHEJIANG OCEAN UNIV
LAMP primer composition and kit for detecting swine acute diarrhea syndrome coronavirus by using microfluidic chip technology
InactiveCN110982937AQuick checkInstant detectionMicrobiological testing/measurementAgainst vector-borne diseasesAcute diarrheaNucleic acid detection
The invention belongs to the field of nucleic acid detection and diagnosis, and in particular relates to a loop-mediated isothermal amplification (LAMP) primer composition and kit for detecting a swine acute diarrhea syndrome coronavirus (SADS-CoV) by using a microfluidic chip technology. In order to solve the defect of time-consuming, laborious and high-cost detection of a swine acute diarrhea syndrome coronavirus, LAMP primers are designed according to a relatively conservative M gene of the SADS-CoV, after screening is performed, the LAMP primer composition for detecting the swine acute diarrhea syndrome coronavirus is obtained. The invention also provides the kit combining the microfluidic chip technology and an LAMP technology and a detection method of the SADS-CoV by using the kit. The composition and kit are applied to detection of the swine acute diarrhea syndrome coronavirus, and can realize the rapid and immediate detection of the swine acute diarrhea syndrome coronavirus, thereby solving the defect of time-consuming, laborious and high-cost detection of the swine acute diarrhea syndrome coronavirus; and the composition and kit improve the detection sensitivity and specificity, reduce labor and equipment costs, and shorten the detection cycle.
Owner:SOUTH CHINA AGRI UNIV
Large-diameter inner hole measuring tool
PendingCN106500569AEasy to detectInstant detectionMechanical diameter measurementsMicrometerEngineering
The invention discloses a large-diameter inner hole measuring tool. The large-diameter inner hole measuring tool comprises an outer-diameter micrometer, an adjusting part, a measuring part and a displaying part. The adjusting part and the displaying part are connected with the measuring part. The adjusting part comprises an adjusting sleeve, a locking sleeve, a first adjusting sleeve joining-in end and a retractable part. The measuring part comprises a main barrel body part, a measuring barrel, a measuring rod, a spring, a balance block and a positioning pin. The displaying part comprises a gauge lining bar, a gauge clamping barrel, a dial gauge and a clamp. When a user measures, an accurate size of an inner diameter of a large hole can be directly, instantly and accurately detected, without the need of a hand feeling; meanwhile, measuring errors caused by the fact that experience of the users is inconsistent are also reduced; and a measuring blank of an inner diameter dial gauge in a range of phi450mm-phi5000mm is filled up.
Owner:岳阳大陆激光技术有限公司
Salmonella immunochromatography test strip based on low-noise excitation type fluorescent mark
InactiveCN104459128AImprove signal-to-noise ratioHigh sensitivityFluorescence/phosphorescenceLow noiseSalmonella immunization
The invention relates to a salmonella immunochromatography test strip based on a low-noise excitation type fluorescent mark and application of the salmonella immunochromatography test strip. A low-noise excitation type fluorescent dye is used as a mark and an immunochromatography technology is adopted to prepare the immunochromatography test strip targeting salmonella; when the salmonella immunochromatography test strip is used for detecting, a special portable type low-noise excitation type fluorescent scanner is used for scanning a quality control line and a detection line respectively to detect a line fluorescence intensity detection value so that the qualitative and quantitative detection of a sample is realized; and the test strip is applicable to instant detection of salmonella in foods and pathological samples. The salmonella immunochromatography test strip has the characteristics of rapidness, high sensitivity, qualitativeness and accurate quantitativeness and portability.
Owner:BEIJING ENTRY EXIT INSPECTION & QUARANTINE BUREAU INSPECTION & QUARANTINE TECH CENT +1
Group A rotavirus chromatography test paper strip based on low-noise excitation fluorescent label
InactiveCN104502608AImprove signal-to-noise ratioHigh sensitivityBiological testingLow noiseQuantitative determination
The invention belongs to the field of food detection, and particularly relates to a group A rotavirus chromatography test paper strip based on low-noise excitation fluorescent label as well as a preparation method and application of the chromatography test paper strip. According to the invention, quantum dots are adopted as the label, the immunochromatography technology is adopted, so that the chromatography test paper strip targeting to group A rotavirus is prepared, during detection, a special portable low-noise excitation fluorescent scanner is adopted for scanning a quality control line and a detection line, and qualitative and quantitative determination of a sample is realized through a fluorescence intensity detection value of the detection line. The test paper strip is used for immediate detection of group A rotavirus in food and a pathological sample, and has the advantages of rapidness, high sensitivity, qualitative, accurate and quantitative detection, and portability.
Owner:BEIJING ENTRY EXIT INSPECTION & QUARANTINE BUREAU INSPECTION & QUARANTINE TECH CENT +2
Colloidal gold test card for quickly testing ergot alkaloid and preparation method thereof
InactiveCN102707047AEasy to carrySimple and fast operationMaterial analysisGlass fiberNitrocellulose
The invention discloses a colloidal gold test card for quickly testing ergot alkaloid and a preparation method thereof. A test paper strip is packaged in a shell of the test card; a sample adding hole and an observation hole are formed on the shell of the test card; the test paper strip comprises a base plate; a piece of water-absorbing paper, a nitrocellulose membrane, a glass fiber membrane and a sample pad are arranged on the base plate in turn; the glass fiber membrane is soaked with an ergot alkaloid antibody marked by colloidal gold; and two lines of envelope antigen ergotic acid-OVA and goat anti-rabbit antibody are sprayed on the nitrocellulose membrane. The colloidal gold test card provided by the invention is conveniently taken and conveniently operated; the colloidal gold test card is used for on-site semi-quantitatively testing raw grains and the content of the ergot alkaloid in the product of the raw grains; the testing accuracy is high and the specificity is strong; the test for the ergot alkaloid is no longer limited by professionals in a professional institute; and all livestock farms, grain purchasing stations, all-level check quarantine inspection departments and consumers can test in real time.
Owner:南通市产品质量监督检验所 +1
Antibacterial type food freshness indication material capable of detecting carbon dioxide and preparation method thereof
ActiveCN108760732AGuarantee factory safetyGrowth inhibitionMaterial analysis by observing effect on chemical indicatorCalcium hydroxideBromothymol blue
The invention discloses an antibacterial type food freshness indication material capable of detecting carbon dioxide. The antibacterial type food freshness indication material is prepared from a membrane solution A, a membrane solution B and a membrane solution C in parts by weight according to a ratio of 2 to (1 to 1.5) to (0.1 to 0.3). The antibacterial type food freshness indication material isprepared by the steps of blending the membrane solution A, the membrane solution B and the membrane solution C, performing ultrasonic and heating treatment, paving on a carrier, performing vacuum drying operation and peeling a membrane to obtain the antibacterial type food freshness indication material. The membrane solution A is prepared from the following raw materials of starch, titanium dioxide dispersion liquid, calcium hydroxide, glycerin, urea, xanthan gum and water; the membrane solution B is prepared from the following raw material of a polyvinyl alcohol solution; the membrane solution C is prepared from the following raw materials of a methyl red solution and a bromothymol blue solution. The material can inhibit growth of bacteria in food and can detect food freshness. The invention further provides a preparation method of the antibacterial type food freshness indication material capable of detecting carbon dioxide.
Owner:JILIN AGRICULTURAL UNIV
Biosensor for detecting okadaic acid and preparation method
InactiveCN104977332AStrong specificityImprove accuracyMaterial electrochemical variablesInhibitory effectElectric signal
The invention relates to a biosensor for detecting okadaic acid. The biosensor comprises an insulated substrate and an electrode system, wherein the electrode system is formed on the substrate and comprises a working electrode, a reference electrode and a counter electrode; and a biological enzyme layer is fixed on the working electrode and comprises protein phosphatase 2A (PP2A enzyme) and an electron transport complex. The invention further relates to a preparation method of the biosensor, and mainly relates to fixation of the PP2A enzyme. Compared with the prior art, the biosensor taking the PP2A enzyme as a fixed enzyme layer is prepared by utilizing the inhibition effect of okadaic acid to the activity of the PP2A enzyme; the content of okadaic acid in a sample is determined according to the activity inhibiting degree of the PP2A enzyme, namely, the concentration of okadaic acid in the sample is converted into an electric signal to be detected, and therefore, the detection result is visual; simultaneously, by virtue of the specificity and the high efficiency of PP2A enzyme catalytic reaction, the detection process is high in specificity, the accuracy rate of a detection structure is high, the detection period is short, and instant and rapid detection requirements can be met.
Owner:ZHEJIANG OCEAN UNIV
Low abundance hormone detection method and device
ActiveCN106199013ALow costQuick checkMaterial analysis by electric/magnetic meansBiological testingElectrochemical detectionElectrophoresis
The invention discloses a low abundance hormone detection method and device. A device for executing detection is provided with a multi-layer structure. Electrophoresis through hole regions of each layer structure are communicated to form an electrophoresis channel. The method comprises the steps that a trailing electrolyte and to-be-detected liquid are dropped on the surface of a top layer of the device; a leading electrolyte is dropped on the surface of a bottom layer of the device; electrophoresis is started by applying direct current voltage between the top layer and the bottom layer, so that the trailing electrolyte and the to-be-detected liquid are migrated in the direction of the bottom layer along the electrophoresis channel, and the leading electrolyte is migrated in the direction of the top layer along the electrophoresis channel; during electrophoresis, when to-be-detected hormone in the to-be-detected liquid is migrated and gathered on an enrichment layer of the device, and electrophoresis is stopped by removing the direct current voltage applied between the top layer and the bottom layer; the to-be-detected hormone gathered on the enrichment layer is moved to a detection region of a detection layer of the device, and electrochemical detection is performed on the detection region, and the concentration of the to-be-detected hormone is determined.
Owner:PEKING UNIV FIRST HOSPITAL +1
Disulfide bond detection method and disulfide bond-containing sputum detection kit
ActiveCN112304930AHigh sensitivityImprove featuresMaterial analysis by observing effect on chemical indicatorDisulfide bondingPotassium cyanide
The invention provides a disulfide bond detection method and a disulfide bond-containing sputum detection kit. The disulfide bond detection method comprises the following steps of mixing a sample to be detected with a buffer system and iron powder, and then detecting disulfide bonds in the sample by developing potassium ferricyanide, wherein the buffer system is a buffer solution of divalent cations, and the pH value of the buffer system is 5.8-6.4. The method disclosed by the invention is simple and easy to implement, capable of completing detection within 10 minutes, clear and easy to judgein result, high in sensitivity and good in specificity and repeatability, and reagents used in the method are all conventional reagents and have very good safety. The kit disclosed by the invention can be stored for a long time, is convenient to carry and use, can be sealed to protect the environment after being used, and is suitable for sputum real-time collection and rapid real-time detection (POCT).
Owner:ZHEJIANG JFK BIOLOGICAL TECH
Method for detecting lens module performance
Owner:HONG FU JIN PRECISION IND (SHENZHEN) CO LTD +1
Clenbuterol hydrochloride micromolecule hapten high-sensitivity detection test paper based on nano antibody, and preparation method thereof
ActiveCN111220802AHigh affinityInstant detectionSerum immunoglobulinsImmunoglobulins against animals/humansBovine serum albuminPolyclonal antibodies
The invention discloses clenbuterol hydrochloride micromolecule hapten high-sensitivity detection test paper based on a nano antibody, and a preparation method of the clenbuterol hydrochloride micromolecule hapten high-sensitivity detection test paper. According to the clenbuterol hydrochloride micromolecule hapten high-sensitivity detection test paper, a gold-labeled pad in the detection test paper adsorbs a colloidal gold nanoparticle labeled anti-clenbuterol hydrochloride small molecule hapten nano antibody Nb; a detection line T on a detection membrane is a conjugate BSA-CL blot of clenbuterol hydrochloride micromolecule CL and bovine serum albumin BSA, and a quality control line C on the detection membrane is an anti-BSA protein rabbit-derived polyclonal antibody pAb blot. The invention further specifically discloses a preparation method of the clenbuterol hydrochloride micromolecule hapten high-sensitivity detection test paper based on the nano antibody. The clenbuterol hydrochloride micromolecule hapten high-sensitivity detection test paper based on the nano antibody prepared by adopting the preparation method is simple and convenient in detection operation, high in specificity, high in sensitivity, capable of rapidly giving a visual result and easy to popularize and apply in life practice.
Owner:XINXIANG MEDICAL UNIV
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