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Redox hydrosol, and making method and application of integrated microbial electrode used for rapid detection of biotoxicity of water

A technology for microbial electrodes and water organisms, which is used in measurement devices, material analysis by electromagnetic means, instruments, etc., can solve the problems of large consumption of electronic mediators, contamination and influence of test samples or reference electrodes, etc., and shorten the detection time. Time, ease of instrumentation and field testing, effect of reduced consumption

Active Publication Date: 2014-08-27
TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Patent No. 200810036317.5 of the Chinese patent "Preparation Method of Microbial Electrode for Water Quality Toxicity Biosensor" discloses a method of using double-sided adhesive tape to prepare microbial sensor electrodes. The electrodes produced by this method only contain microbial flora. During the detection process of the sensor It still needs to rely on the electron mediator dispersed in the solution, and the consumption of the electron mediator dispersed in the solution is relatively large, which inevitably has certain pollution and influence on the detection sample or reference electrode

Method used

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  • Redox hydrosol, and making method and application of integrated microbial electrode used for rapid detection of biotoxicity of water
  • Redox hydrosol, and making method and application of integrated microbial electrode used for rapid detection of biotoxicity of water
  • Redox hydrosol, and making method and application of integrated microbial electrode used for rapid detection of biotoxicity of water

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1. Preparation of a redox hydrosol:

[0034] Dissolve gelatin in water to obtain a solution with a mass concentration of 50mg / ml; adjust the pH to 4, add aminopropyltrimethoxysilane (APTMOS), the mass fraction of APTMOS in the solution is 1%, and stir at 50℃ until it forms Uniform composite sol; mix the composite sol with 0.04mol / L benzoquinone sol (volume ratio 4:1), and stir for 12 hours until a redox hydrosol is formed;

[0035] 2. Preparation of integrated microbial electrode for rapid detection of water biological toxicity:

[0036] (1) Microbial broth: E. coli is used as the tested microorganism and inoculated in a liquid medium. E. coli liquid medium composition (mass fraction): 0.3% beef extract, 1% peptone, 0.5% sodium chloride. Cultivate for 16 hours, centrifuge to clean the wet bacteria, disperse in phosphate buffer, and store at 4°C;

[0037] (2) Take the synthetic redox hydrosol and mix it evenly with the E. coli bacteria liquid in a volume ratio of 1:1 to obtain...

Embodiment 2

[0045] The operating method and steps are the same as in Example 1. The only change is that Cu is added to the system during the monitoring and detection of the biological toxicity of the water body using the integrated microbial electrode 2+ And Zn 2+ Mixture, Cu 2+ And Cd 2+ Mixture, Zn 2+ And Cd 2+ Instead of Hg 2+ Solution (the concentration of each heavy metal in the mixture is 40μg / ml). The results show that the response current drops significantly after adding different binary heavy metal mixtures. The inhibition rate of the three groups of binary heavy metal mixtures is calculated as image 3 As shown, the order of toxicity is Cu 2+ -Zn 2+ > Zn 2+ -Cd 2+ > Cu 2+ -Cd 2+

Embodiment 3

[0047] 1. Preparation of a redox hydrosol:

[0048] Dissolve gelatin in water to obtain a solution with a mass concentration of 10mg / ml; adjust the pH to 3, add aminopropyltrimethoxysilane (APTMOS), the mass fraction of APTMOS in the solution is 0.5%, and stir at 60℃ until uniform The composite sol; take the concentration of 0.01mol / L menadione aqueous solution and mix with the prepared composite sol (volume ratio is 1:10), stir for 12 hours, until the redox hydrosol is formed.

[0049] 2. Integrated microbial electrode for rapid detection of water biological toxicity and its preparation:

[0050] (1) Microbial broth: E. coli is used as the tested microorganism and inoculated in a liquid medium. E. coli liquid medium composition (mass fraction): 0.3% beef extract, 1% peptone, 0.5% sodium chloride. Cultivate for 16 hours, centrifuge to clean the wet bacteria, disperse in phosphate buffer, and store at 4℃

[0051] (2) Take the synthetic redox hydrosol and mix it evenly with the E. col...

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Abstract

The invention relates to a redox hydrosol. The redox hydrosol is prepared through the steps of preparing an inorganic-organic composite sol by using a polymer sol and an aminosilane coupling agent, and mixing the composite sol with an electronic mediator. The invention also relates to a making method of an integrated microbial electrode used for the rapid detection of the biotoxicity of water, and an application of the integrated microbial electrode. Microbes and the electronic mediator are fixed on an electrode through the prepared redox hydrosol to make the integrated microbial electrode, and the integrated microbial electrode can be applied in the rapid detection and instant monitoring of the biotoxicity of water. An electrochemical sensor can system constructed by using the integrated microbial electrode made in the invention can instantly monitor the water organism toxicity change and rapidly detect the water organism toxicity value, can realize instant, continuous and rapid detection, and has the characteristics of high analysis sensitivity, low cost, simple operation, portability and the like.

Description

Technical field [0001] The invention belongs to the technical field of microbial sensors, and specifically relates to a preparation method and application of a redox hydrosol and an integrated microbial electrode for rapid detection of water biological toxicity. Background technique [0002] With the development of modern industry, increasing environmental pollution has caused a great impact on the water ecosystem, and toxicity testing on it has become an important part of evaluating the quality of water environment. At present, the methods used for pollutant toxicity testing mainly include physicochemical methods and biological methods. Traditional physical and chemical methods can accurately and quantitatively analyze the type and content of a certain pollutant or a certain type of pollutant; but these methods cannot directly and comprehensively reflect the comprehensive impact of toxic substances on the environment. The biological method is to detect the changes of toxic subs...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327
Inventor 只金芳李久铭钱俊
Owner TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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