50 results about "Okadaic acid" patented technology

The present invention provides cell permeable NF-κB inhibitors consist of a polypeptide derived from the p65 subunit of NF-κB and a protein transduction domain derived from antennapedia third helix sequence. The inhibitor suppressed NF-κB activation induced by TNF, LPS, IL-1, okadaic acid, PMA, H2O2 and cigarette smoke condensate. NF-κB-regulated reporter gene expression induced by TNF, TNFR1, TRADD, TRAF2, NIK, IKK and p65 was suppressed by the inhibitor. The inhibitor enhanced TNF- and chemotherapeutic agent-induced apoptosis. Overall these results demonstrate a NF-κB inhibitor that can selectively inhibit NF-κB activation induced by various inflammatory stimuli, downregulate NF-κB mediated gene expression and upregulate apoptosis.

The invention provides an ELISA detection method of red-tide algae toxin okadaic acid (OA) and has the advantages of quickly, simple and sensitive detection of the OA content and low cost. The ELISA detection method of the invention comprises the following steps: (1) diluting coated antigens by using carbonate buffer solution to 1:2000, coating ELISA plates; (2) washing the plates for three times and sealing the plates; (3) dumping the blocking solution, washing the plates, separately stirring and mixing OA standard solution which is diluted to series concentrations and shellfish extract liquid with isometric anti-OA monoclonal antibody which is diluted to 1:10,000 evenly to react; (4) dumping the residual liquid, washing the plates, adding goat anti-rat IgG antibody labeled with HRP, diluting the solution to 1:5000 with each pore containing 100mu l of solution, keeping temperature for 1h at 37 DEG C; (5) dumping the residual liquid, washing the plates, adding developer and measuring the OD450 value of each pore.

The invention relates to a biosensor for detecting okadaic acid. The biosensor comprises an insulated substrate and an electrode system, wherein the electrode system is formed on the substrate and comprises a working electrode, a reference electrode and a counter electrode; and a biological enzyme layer is fixed on the working electrode and comprises protein phosphatase 2A (PP2A enzyme) and an electron transport complex. The invention further relates to a preparation method of the biosensor, and mainly relates to fixation of the PP2A enzyme. Compared with the prior art, the biosensor taking the PP2A enzyme as a fixed enzyme layer is prepared by utilizing the inhibition effect of okadaic acid to the activity of the PP2A enzyme; the content of okadaic acid in a sample is determined according to the activity inhibiting degree of the PP2A enzyme, namely, the concentration of okadaic acid in the sample is converted into an electric signal to be detected, and therefore, the detection result is visual; simultaneously, by virtue of the specificity and the high efficiency of PP2A enzyme catalytic reaction, the detection process is high in specificity, the accuracy rate of a detection structure is high, the detection period is short, and instant and rapid detection requirements can be met.

The invention discloses a method for detecting diarrhea shellfish toxin, which comprises the following steps: A) establishing a standard curve of depolymerization of okadaic acid (OA) induced HL-7702 hepatocyte F-actin; B) carrying out the calculation on the concentration of the OA according to the standard curve of the F-actin depolymerization of the HL-7702 hepatocyte, establishing a detection method of the hepatocyte F-actin of the diarrhea shellfish toxin to determine the possible detection limit range; C) selecting one or more of components (STX, DA and YTX) which are possibly coexist with the diarrhea shellfish to act on the HL-7702 hepatocyte, determining the specificity of the method for detecting the OA content by detecting the degree of damaging the polymerizing power of the HL-7702 hepatocyte F-actin by the above components. The detection method of the invention has the advantages: (1) taking cells as experimental subjects to avoid the using of experimental animals and conforming to the rule of 3R; (2) having high sensitivity; (3) having good specificity; (4) having good repeatability; and (5) having simple and convenient sample extraction and low matrix effects.

The invention particularly relates to a method for establishing a zebrafish senile dementia model and application. A glucose culture solution and an okada acid culture solution are included,the glucose culture solution and the okada acid culture solution are alternately used for culturing zebrafishes, and a single culture time of the glucose culture solution orthe okada acid culture solution is 22-26 hours. The method disclosedis the first methodwhich alternately cultures the zebrafishes with a specific concentration of the glucose culture solution and the okada acid culture solution, zebrafish senile dementia-like symptoms can be quickly and effectively caused, thereby greatly reducing the dosage of okada acid, and experimental resources are saved.

The invention discloses a preparation method of an okadaic acid toxin molecularly imprinted-quantum dot polymer and an application. The preparation method comprises the steps of taking okadaic acid as a template molecule and adding a quantum dot fluorescent nanomaterial; and initializing polymerization in the presence of a cross-linking agent tetraethylortho silicate and a functional monomer, and then removing the template molecule in the obtained polymer by adopting an ultrasonic-assisted extraction method to obtain the okadaic acid toxin molecularly imprinted-quantum dot polymer. The application method comprises the steps of sampling a sample extract solution to an okadaic acid Spin solid-phase extraction column; centrifuging, collecting an eluent and filtering the eluent through a 0.22-micron filter membrane; and measuring the content of okadaic acid toxin by adopting an LC-MS / MS. The method has the advantages of being high in sensitivity, good in stability, high in selectivity, simple in method and convenient to operate, and the targets of simply and efficiently achieving selective separation, purification an enrichment of okadaic acid saxitoxin in a shell sample are achieved.

The invention relates to a chemiluminesent immunoassay kit for detecting okadaic acid, belonging to the technical field of laboratory detection kits. The chemiluminesent immunoassay kit comprises a latching head, a kit cover, a kit body, a cut sponge, a buckling groove, a drawer, an overturning cover, an elisa (enzyme-linked immuno sorbent assay) plate support, cylindrical pins, a support, a reagent bottle support, a groove and a 96-well elisa plate, wherein the latching head is arranged on the kit cover which is connected with the kit body, the buckling groove is arranged on the kit body, the drawer is arranged under the kit body, the elisa plate support and the reagent bottle support are arranged in the kit body, the overturning cover is connected with the elisa plate support, the cut sponge is arranged on the bottle surface inside the elisa plate support, the 96-well elisa plate is arranged on the cut sponge in the elisa plate support, cylindrical pins are arranged on left and right side surfaces of the elisa plate support, the reagent bottle support is arranged under the elisa plate support, cylindrical pins are arranged on left and right side surfaces of the reagent bottle support, the folding design of the elisa plate support and the reagent bottle support can achieve firmness between the elisa plate support and the reagent bottle support and ensures that a reagent bottle can be taken conveniently.