Preparation of paper-based coupling enhanced Raman sensor and application of same in detection of okadaic acid

A kind of okadaic acid and sensor technology, applied in the field of paper-based coupling enhanced Raman sensor, can solve the problems of poor flexibility, unsuitable for large-scale processing and preparation, on-site analysis and application, time-consuming, etc., and achieve the effect of improving selectivity

Active Publication Date: 2020-01-17
UNIV OF JINAN
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Problems solved by technology

However, the above-mentioned substrates are not suitable for large-scale processing and on-site analysis applications due to high cost, complicated and time-consuming preparation process, and poor flexibility.

Method used

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  • Preparation of paper-based coupling enhanced Raman sensor and application of same in detection of okadaic acid
  • Preparation of paper-based coupling enhanced Raman sensor and application of same in detection of okadaic acid

Examples

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Embodiment 1

[0023] Application of a paper-based surface-enhanced Raman sensor based on complementary DNA strands in the detection of haicic acid in Daejeon:

[0024] (1) Use the graphics software Adobe Illustrator CS6 to draw each functional area, and then use solid wax to print on chromatographic paper to simultaneously manufacture 50 preliminary paper chip models. Bake the wax printing graphics, so that the wax saturates the back of the paper and forms hydrophobic areas on both sides of the chromatography paper;

[0025] (2) In the functional area of ​​the paper chip model obtained in step (1), use the gold seed solution to modify the surface of the paper base with gold nanoparticles. After incubation, the paper fibers are catalyzed in situ to form an orderly structure, dense arrangement, and interpenetration. Nano-gold particles on the upper and lower sides of the fiber to obtain a nano-gold-paper-based solid phase substrate, that is, a "hot spot" primary enhanced SERS paper substrate;...

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Abstract

The invention provides a paper-based coupling enhanced Raman (SERS) sensor based on aptamer specific capture performance and a preparation method thereof, and provides high-specificity analysis of okadaic acid in marine water and marine products by means of the paper-based coupling enhanced Raman sensor. The application of the paper-based coupling enhanced Raman sensor is implemented by the stepsof: assembling dens and ordered nano gold particles on the surface of paper fibers in a reduction induced mode so as to construct a surface plasma resonance nano gold paper SERS substrate; synthesizing a three-dimensional space dendritic 3D silver material in situ, so as to construct a high-hotspot-density electromagnetic field enhanced SERS coupling double-layer; and introducing complementary DNAchains to establish a specific capture-surface coupling enhanced Raman analysis interface, so as to realize rapid and precise Raman spectrum analysis. The purpose of field detection is achieved, thepreparation method is simple, the cost is low, large-scale preparation can be achieved, and theoretical and technical support is provided for seawater treatment.

Description

technical field [0001] The invention relates to a paper-based coupling-enhanced Raman sensor based on the specific capture performance of aptamers, in particular to a paper-based surface-enhanced Raman sensor with complementary DNA strands and its preparation method and its application in marine water bodies and marine shellfish Application in the detection of okadaic acid. Background technique [0002] Okadaic acid is a lipophilic, thermostable polyether diarrhea algal toxin. Food poisoning incidents caused by okadaic acid pollution occurred in Japan and the United Kingdom in 1976 and 1997 respectively. Although there is no report of human death due to okadaic acid poisoning, it has shown its promoting effect on tumors, as well as mutagenic and immunotoxin effects. However, algae containing okadaic acid are almost all over the world's major oceans. Therefore, the development of an on-site rapid detection method that can realize okadaic acid in marine water and marine she...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 赵珮妮于京华刘焕英
Owner UNIV OF JINAN
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