Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Electrochemical detection method of okadaic acid in shellfish

A technology of Datian soft sponge acid and detection method, applied in the direction of electrochemical variables of materials, etc., can solve the problems of expensive, cumbersome steps, complicated instruments, etc., and achieve the effects of fast response, high sensitivity and good specificity

Active Publication Date: 2014-04-09
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
View PDF1 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved in the present invention is to provide an electrochemical detection method for okadaic acid in shellfish, so as to overcome the deficiencies in the existing technology for detecting the shellfish toxin okadaic acid, such as complex, expensive, and cumbersome steps, etc. shortcoming

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Electrochemical detection method of okadaic acid in shellfish
  • Electrochemical detection method of okadaic acid in shellfish
  • Electrochemical detection method of okadaic acid in shellfish

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0030] The technical solution of the present invention will be further explained by the following examples, but the protection scope of the present invention is not limited in any form by the examples.

[0031] An electrochemical detection method for okadaic acid in shellfish, comprising the following steps:

[0032] (1) Sample pretreatment: Weigh 1g shellfish sample into a 50mL centrifuge tube, add 8mL80% methanol aqueous solution, extract homogeneously at 10000r / min for 2min, then centrifuge at 8000r / min for 5min, transfer the supernatant to a 10mL colorimetric tube , and dilute to 10 mL with methanol. Pipette 1 mL of extraction liquid nitrogen and blow it to nearly dry, dilute to 1 mL with 10 mmol / L PBS (pH 7.4), sonicate for 30 s to fully dissolve the residue, and pass the solution through a 0.45 μm filter membrane before testing.

[0033] (2) Synthesis of nano-gold: take 50mL HAuCl 4 (0.01%), heat to boil, and quickly add 1.5mL of 1% trisodium citrate aqueous solution u...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an electrochemical detection method of okadaic acid in shellfish, which belongs to the technical field of electrochemical detection. The electrochemical detection method comprises the following steps: firstly, electro-polymerizing a thionine-methylene blue composite film on the surface of a bare gold electrode, and sequentially modifying nanogold and an antibody on the electrode surface through electrode surface modification technology to prepare an electrochemical immunosensor; and then, applying the prepared electrochemical immunosensor to electrochemical detection of okadaic acid in shellfish. A peak current signal is amplified by means of a synergistic effect of the thionine-methylene blue composite polymer film and the nanogold to improve the sensitivity of the sensor; the okadaic acid with special molecular structure is specifically combined with the antibody to form an immune product existing in the form of anion, and the immune product can block the electron transfer property of the electrode surface to achieve non-labeled electrochemical detection. The method disclosed by the invention is used for measuring okadaic acid in shellfish samples, the detection is fast, the sensitivity is high and the result is accurate and reliable.

Description

technical field [0001] The invention belongs to the technical field of electrochemical detection, in particular to an electrochemical detection method for okadaic acid in shellfish. Background technique [0002] Okadaic acid (OA) is a polycyclic polyether fat-soluble shellfish toxin and is the main component of marine diarrheal shellfish toxins. OA can be enriched in filter-feeding marine organisms such as shellfish through the food chain, causing gastrointestinal dysfunction in consumers, and poisoning symptoms such as diarrhea, vomiting and abdominal pain. Studies have confirmed that OA and its derivatives, fin algae toxins, can inhibit the activity of protein phosphatases, and are a class of potential tumor-promoting factors, showing a trend of increasing occurrence frequency, expanding distribution area, and increasingly serious harm. The major food and ecological security issues of common concern are also the main goals of developed countries in formulating monitoring ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N27/48
Inventor 郭萌萌谭志军吴海燕李兆新王联珠翟毓秀
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com