Fluorescent nucleic acid aptamer sensor for detecting okadaic acid, preparation method of fluorescent nucleic acid aptamer sensor and method for detecting okadaic acid by using fluorescent nucleic acid aptamer sensor

Abstract

The invention discloses a fluorescent nucleic acid aptamer sensor for detecting okadaic acid, a preparation method of the fluorescent nucleic acid aptamer sensor and a method for detecting okadaic acid by using the fluorescent nucleic acid aptamer sensor, and belongs to the technical field of harmful substance detection. According to the invention, the fluorescent nucleic acid aptamer sensor for detecting the okadaic acid is formed by self-assembly of a nucleic acid aptamer which is marked by a fluorophore at the 5' end and can specifically recognize the okadaic acid and graphene oxide. When the fluorescent nucleic acid aptamer sensor is used for detecting okadaic acid, the operation steps are simple and clear, the cost is low, the specificity is good, the detection range is between 0.5 ng / mL and 200 ng / mL, and the sensitivity is high.

Description

technical field [0001] The invention belongs to the technical field of harmful substance detection, and in particular relates to a fluorescent nucleic acid aptamer sensor for detecting okadaic acid, a preparation method thereof and a method for using the sensor to detect okadaic acid. Background technique [0002] Okadaic acid is a low-molecular-weight diarrheal shellfish toxin in shellfish, which can cause nausea, vomiting, and diarrhea in those who eat it by mistake; it has a strong inhibitory effect on the activity of protein phosphatase in the cytoplasm and affects normal cell activities; It even has a direct toxic effect on human cells, interferes with the DNA repair process, changes gene expression, and induces tumor formation. Okadaic acid is fat-soluble and thermally stable, and it is difficult to remove it through common processing techniques during processing. It may even be due to dehydration of shellfish and conversion of esterified toxins to free states, resulti...

AI Technical Summary

Problems solved by technology

[0005] In view of the problems in the prior art that detection reagents are easily inactivated, detection samples require pretreatment, cumbersome steps, low sensitivity, high requirements for instruments and equipment, difficulty in large-scale use and meeting the requirements of on-site detection, the purpose of the present invention is to provide a Graphene is the fluorescent aptasensor of fluorescence quencher, and the method for using this sensor to detect okadaic acid

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