117results about How to "Achieve qualitative" patented technology

The invention relates to a qualitative and quantitative analysis method for polyoses, which comprises the following steps: (1) the zymohydrolysis and the verification of the polyoses: measuring the content of a polyoses water solution by a colorimetric method; (2) comparing the change of a polyoses mapping before and after zymohydrolysis by HPSEC-ELSD analysis; (3) saccharide ingredient direct HPLC analysis of a polyoses zymohydrolysis solution or HPLC analysis after pre-column derivatization: identifying polyoses mapping and chromatogram peaks by taking a standard monosaccharose, a uronic acid, a disaccharide, and the like as contrasts and taking a chromatogram peak mass-to-charge ratio as reference, and using stable characteristic sections as a quantitative analysis index; and (4) realizing the qualitative and quantitative analysis of the polyoses by establishing polyoses zymohydrolysis responding characteristics and polyoses zymohydrolysis mapping which are based on structural information through the independent or combining application of the steps. The qualitative and quantitative analysis method can be used for the identification and quantitative measurement of the polyoses of traditional Chinese medicine, such as panax, panax pseudoginseng, gen-seng, aweto, cordyceps, ganoderma lucidum, fomes japonica, milk veteh, angelica, and the like and provides an effective method for controlling the quality of the polyoses and the products thereof.

The invention relates to functional nanometer particle composite non-crosslinking microsphere powder, and a preparation method and application of the functional nanometer particle composite non-crosslinking microsphere powder. The functional nanometer particle composite non-crosslinking microsphere powder contains functional nanometer particle composite non-crosslinking microspheres; the functional nanometer particle composite non-crosslinking microspheres contain functional nanometer particles and polymers, average particle size is 0.1micron to 20micron, and particle size distribution variable coefficient is less than or equal to 9.1%. The preparation method is a combination of a membrane emulsification technique and a solvent volatilization method. The invention also relates to a biometric probe and application of the biometric probe based on the above composite non-crosslinking microsphere powder. The preparation method has the advantages that the functional nanometer composite non-crosslinking microsphere powder which is uniform in particle size can be prepared; the prepared functional nanometer composite non-crosslinking microspheres belong to micron order, the particle size variable coefficient is small, monodispersity is good, and performance is excellent; and the prepared functional nanometer composite non-crosslinking microspheres have broad application prospect in biometric and biomedical fields and the like.

An electric automobile charging and replacing station fire risk data evaluation method includes the steps: numerically simulating actual acquired data of fire scene main structures of a distribution room, a charging and replacing room and a monitoring room in an electric automobile charging and replacing station by a modeling tool to obtain a large number of simulated data, and screening or exhausting the fire development condition of a single room after catching fire and influence factors of the fire on the whole charging and replacing station; determining a data evaluation range; building a fire risk data hierarchy structure model; calculating the weight of each factor by a hierarchy analysis method; determining fire risk levels; judging whether the fire risk levels meet safety requirements or not, if the fire risk levels do not meet the safety requirements, calculating improving suggestion data according to fire risk and harmfulness obtained in the first step, returning to the fourth step according to the proposed suggestion data, reconstructing a judgment matrix, calculating the weight and performing evaluation until meeting evaluative criteria. Reliable data can be provided, and prediction accuracy is improved.

The invention relates to a method for detecting oligosaccharides in breast milk. The method includes a step of breast milk sample pretreatment, namely taking a breast milk sample, centrifugally removing upper fat, taking lower liquid, adding alcohol solvent, freezing for 5-15min at a temperature ranging from -40 DEG C to -100 DEG C, centrifuging and taking supernatant. The invention further provides a method for qualitative and quantitative detection of oligosaccharides in the pretreated breast milk according to a liquid chromatography-mass spectrometry method. The method for simultaneous detection of various oligosaccharides in non-reducing breast milk by liquid chromatography-mass spectrometry is established and has advantages of effectiveness, quickness and simplicity in pretreatment. AQ Exactive detector is adopted for mass spectrometry, liquid-phase gradient elution parameters and mass spectrometric detection parameters are determined, ion information of non-reducing breast milkoligosaccharides in mass spectrometric detection under a positive ion mode is given, and the method can be well applied to qualitative and quantitative detection of the oligosaccharides in the breastmilk.

The invention discloses a tea leaf classifying method. The tea leaf classifying method comprises the following step of using a vapor phase-ion transfer joint technique to detect volatile organic compounds in different types of tea leaves, wherein detection information is used as classifying basis. The tea leaf classifying method is characterized in that feature information of different types of tea leaves are respectively represented by corresponding ion transfer time and ion strength under different retention time, the method comprises collection of a three-dimension spectrum map, selection of a spectrum map feature area, pre-processing of data and analysis of chemical metering data, so as to classify samples. The tea leaf classifying method has the advantages that the vapor phase-ion transfer joint technique can be widely applied to the field of quick analysis of qualities of foods and agricultural products containing complicated systems; the analysis time is short, the resolution is high, the sensitivity is high, the non-destructive detection can be performed, and the tea leaf classifying method can be applied to classify the types of tea leaves.

The invention discloses a method for quickly detecting copper ions in a solution. The method comprises the following steps of: reacting a reducible water-soluble compound with a water-soluble compound which contains precious metal in a polyhydroxy water-soluble compound which is used as a protective agent to obtain a detecting solution containing precious metal nano particles, which are modified by the polyhydroxy water-soluble compound; supplying two parts of the same detecting solutions with equal quantities, adding the solution without the copper ions and a solution to be detected, which are isometric, into the two parts of the detecting solutions respectively, and forming a first mixing solution and a second mixing solution which are corresponding; and judging whether the copper ions exist in the solution to be detected or not according to the color variation of the second mixing solution relative to the color of the first mixing solution or according to the variation of the ultraviolet visible adsorption strength or peak position of the second mixing solution relative to that of the first mixing solution, wherein the copper ions are divalent copper ions. The method disclosed by the invention is simple and is flexible in design, free from a large-scale instrument, high in sensitivity and selectivity, strong in stability, good in reproducibility of the detecting result, and low in cost.

The invention belongs to the technical fields of preparation and application of metal optical function nano materials and in particular relates to a fluorescent silver nanocluster as well as a simple preparation method and application thereof. The fluorescent silver nanocluster is synthesized by reacting soluble silver salt with a stabilizer, wherein the fluorescent silver nanocluster has the mean particle size of more than 1.5nm and less than 2nm; the fluorescence emitted by the fluorescent silver nanocluster has the wavelength of 610nm under excitation of light with the wavelength of 510nm; the emitted fluorescence has high illumination stability. The method is readily available in raw materials, simple in process, convenient to operate, low in biotoxicity and easy to popularize and apply. The synthesized fluorescent silver nanocluster can be applied to the fields of optical imaging, biological markers, biological or chemical sensors and the like, and especially can be used for qualitative and quantitative detection on the activity of alpha-L-fucosidase.

The invention discloses a quick phased array ultrasonic whole-focusing imaging technology, which includes steps of: separating a collected full-matrix data A*NRX*NTX into N two-dimensional sub-matrixdata An*NRX; performing virtual focusing to all sub-matrix data by means of algorithm based on two-dimensional forward / inverse Fourier transform; performing circulation calculation to acquire the two-dimensional focusing images of the first to Nth sub-matrixes; finally fusing the two-dimensional focusing images of all sub-matrixes to form the whole-focusing image. The method is expected to solve aproblem that a phased array ultrasonic fault detector cannot achieve real-time whole-focusing imaging, can quickly and effectively achieve high-precise quantization, positioning and qualification tointernal defects of materials, and has great promotion and application prospect.

The invention provides a method for detecting related substances of ibuprofen or its sodium salt and preparation. The related substances comprise impurities A, B, C, D, E and F. The method comprises (1) preparation of a test sample solution: taking an appropriate amount of ibuprofen or ibuprofen sodium raw materials, putting the materials into a certain volume of a container, dissolving the materials, diluting the solution until a desired volume marked by a scale, shaking the solution, and filtering the solution to obtain a certain concentration of the test sample solution, and (2) sample detection: pouring the test sample solution into a chromatographic instrument, and acquiring a chromatogram map of the related substances separated effectively under chromatographic conditions of use of a chromatographic column containing octadecyl silane chemically bonded silica as a filler having size of 250*4.6mm and pore sizes of 5 micrometers and having a column temperature of 20 to 40 DEG C, use of a mobile phase having a volume ratio of organic phase acetonitrile to a water phase phosphoric acid aqueous solution of 32% to 48% and phosphoric acid content of 0.01 to 0.1% in the phosphoric acid aqueous solution, a flowing rate of 1.0 to 2.3 ml / min, and a detection wavelength of 205 to 225nm. The method can realize effective separation of a variety of impurities.

The invention relates to a fluorescent probe for specifically distinguishing different thiol and belongs to the field of fluorescent probes. A molecular structure of the fluorescent probe is as follows: a formula is shown in the description. A probe molecule has no fluorescent light; after the probe molecule responds with thiophenol, a solution produces blue light and red light; after the probe molecule responds with GSH (glutathione), the solution produces the blue light; after the probe molecule responds with Hcy / Cys, the solution produces the blue light and green light. The probe molecule provided by the invention can be used for distinguishing different types of the thiol and can be used for rapidly and quantitatively detecting different types of the thiol, and has important application value in the fields of biochemistry, environment monitoring and the like.

The invention provides a method for performing nuclear magnetic resonance identification on adulterated non-dairy cream in watery cream. The method comprises the following steps: (1) collecting creamsamples and performing plausibility check on the samples; (2) preparing adulterated cream samples by performing plausibility check on the cream samples and collecting 1H-NMR of the samples; (3) establishing a PLS-DA qualitative model and a PCA-SVM regression quantitative analysis model by adopting 1H-NMR data subjected to data processing; (4) collecting 1H-NMR data of the cream samples with unknown adulteration or not, and performing detection by the established qualitative and quantitative models so as to obtain the identification of the unknown cream samples. According to the method, the accuracy of the modeling sample is guaranteed, the condition that the non-dairy cream is adulterated in the watery cream can be detected rapidly, qualitatively and quantitatively, the technology is reliable, the operation is simple and convenient, the adopted model is high in calculation speed and accurate in identification result, and technological support is provided for quality supervision of baking products such as cream cakes.

The invention provides a real-time fluorescence constant temperature index amplification method. According to the method, an oligonucleotide signal amplification template of a stem-ring-tail structure and marked with fluorescence or an oligonucleotide signal amplification template of a pure linear structure and marked with fluorescence are used, the process of cutting, extending and chain replacing can be executed repeatedly at the constant temperature under the combined action of a nicking agent with one chain of a cut double-chain nucleic acid molecule and a DNA polymerase with chain replacing activity, so that target molecule specific oligonucleotides are amplified in an index form, and fluorescence signals can be released. By means of varieties and abundance of the fluorescence signals released by the signal amplification templates corresponding to the different target molecule specific oligonucleotides, multiple target molecules can be detected in parallel in a single reaction tube, a detection tank or a detection hole, the target molecule specific oligonucleotides can be amplified by 106 times or more, and the technical problem that by means of an existing constant temperature amplification technology, multiple target molecules cannot be detected at the same time in one detecting system can be effectively solved.

The invention discloses an ultra-trace lead-cadmium ion detection method and a detection test strip. The detection method comprises the following steps: (1) acidifying a sample; (2) removing Hg2+ and Zn2+ in the sample; (3) diluting the sample by utilizing a buffer solution to obtain a neutral sample; (4) enabling the neutral sample obtained in the step (3) to be contacted with dithizone to generate a lead-cadmium ion-dithizone complex; (5) adding sulfide solution so as to enable the lead-cadmium ion-dithizone complex to be completely reacted to generate PbS and / or CdS; (6) carrying out silver staining reaction on the mixture containing PbS and / or CdS obtained in the step (5), and determining the concentration of lead and cadmium ions in the to-be-detected sample according to the grey scale of a reaction product. The test strip comprises a substrate film and a dithizone layer, wherein the dithizone layer is compounded onto the substrate film, and the test strip is packaged in a vacuum manner. The detection method and the detection test strip are sensitive inn detection, simple in judgment, capable of reaching the standard of naked eye detection, strong in applicability and stable inn performance.

The invention relates to a nano chemical sensor based on a semiconductor nanowire and capable of supplying power by self and a preparation method thereof. The nano chemical sensor comprises a semiconductor nanowire, wherein the semiconductor nanowire grows on a substrate which is non-conductingg and can not react with an organic chemical molecule; one part of the growing semiconductor nanowire is covered by a seal layer which is non-conductive, not dissolved in an organic chemical solvent and water and does not react with the semiconductor nanowire and electrodes; one of the electrodes is connected with the semiconductor nanowire sealed by the seal layer, and the other electrode is connected with the semiconductor nanowire which is not sealed by the seal layer; when the nano chemical sensor is in contact with a chemical substance, a potential difference can be generated between the part covered by the seal layer and the part which is not sealed, thereby carrying out quantitative and qualitative detection without adding a power supply.

The present invention discloses a mobile surveillance car-based single particle volatile organic compound online mass spectrum detection system and method, the system includes a mobile surveillance car and a volatile organic compound online mass spectrometer, the interior of the mobile surveillance car is provided with a driving room, a workspace area, a functional area and an instrument area, and the volatile organic compound online mass spectrometer is a single-photon ionization flight time mass spectrometer, is placed in the functional area, and is used for qualitative and quantitative real-time online analysis of industrial source volatile organic compound emissions. The mobile surveillance car-based single particle volatile organic compound online mass spectrum detection system can detect the composition and concentration of industrial source volatile organic compounds, a sample can be directly and quickly detected without sample preprocessing, high throughput sample analysis can be achieved, pollutant source volatile organic compound emissions can be detected in crusing mode, and the emissions of the composition and concentration of the volatile organic compounds at higher time resolution over time can be characterized.

The invention relates to a construction method and an application of chemiluminiscence-colloidal gold immunochromatography test paper, which has high sensitivity and is quantitative. The method comprises the following steps: chemiluminiscence and a colloidal gold immunochromatography method are combined, namely the surface of colloidal gold is simultaneously coupled with a detection antibody and an enzyme to form a colloidal gold-enzyme-antibody compound; the colloidal gold-enzyme-antibody compound and a coated antibody are sequentially sprayed on a sample combination cushion and a film; an immunochromatography reaction is carried out and qualitative detection is realized based on color shades of the colloidal gold; quantitative detection is carried out based on the light intensity of an enzyme catalyzed chemiluminiscence reaction so as to construct the chemiluminiscence-colloidal gold immunochromatography test paper; the test paper is applied to the detection of biological macromolecules and micro-molecules. The chemiluminiscence-colloidal gold immunochromatography test paper can simultaneously realize quantitative, qualitative and high-sensitivity detection and is suitable for field detection and domestic individualized diagnosis.

The invention discloses a time-frequency combined rapid full-focusing ultrasonic imaging method, which comprises the following steps of firstly, splitting acquired three-dimensional full-matrix data into N two-dimensional matrix slices, secondly, by means of two-dimensional forward / inverse Fourier transform, achieving received wave field extrapolation on each depth layer on a certain slice step bystep, then, by calculating a delay matrix from each pixel point to a transmitting array element, interpolating the extrapolated time domain signal to realize focusing on the slice, and finally, repeating the phase shift and interpolation operation on the N two-dimensional slices and accumulating to obtain a time-frequency combined full-focus image. The method has the advantages that compared withtraditional time domain full focusing, the method is low in complexity and high in imaging speed, the problem that full focusing is difficult to achieve real-time imaging is expected to be solved, high-precision quantification, positioning and qualitative determination of internal defects of materials can be effectively achieved, and the method has good application and popularization prospects.

The invention relates to the field of electroforming, in particular to a measurement method of the internal stress of an electrical deposition layer. The measurement method comprises the following procedures: dipping a cathode test slice and an anode test slice in a transparent electroplating bath, annealing the anode test slice for 30 to 50 min under the temperature of 600 to 800 DEG C before test to eliminate the residual stress of the anode test slice, rolling out the anode test slice under static press, making the single surface of the anode test slice insulated, measuring the elastic modulus value E and the thickness value t of the anode test slice, and calculating the length L of the anode test slice dipped in electroplating liquid; making the insulated surface of the anode test slice back to the cathode test slice, connecting the cathode test slice and the anode test slice with a power supply, providing a camera outside the transparent electroplating bath, and calculating the displacement Z' of the bottom end of the anode test slice; measuring the thickness of the electroplated anode test slice, calculating the thickness value of the electrical deposition layer, and finally, calculating the internal stress S of the electrical deposition layer according to a formula, i.e. S=(1 / 6)*(Et<2>Z' / dL<2>). In the invention, computer imaging and measurement technique are adopted to accurately measure the displacement of the bottom end of the anode test slice so as to qualitatively and quantificationally measure the internal stress of the electrical deposition layer.

The invention discloses a gas phase chromatography-mass spectrometry detection method of polyoxyethylene nonyl phenyl ether in water, belonging to the technical field of water environment pollutant detection. The detection method comprises the steps of membrane filtration, pre-purification, solid phase extraction, enrichment and elution and comprises the following concrete steps: using an organicsolvent to extract the eluent which is specially treated chemically; then carrying out the gas phase chromatography-mass spectrometry detection of the organic phase; comparing the detection result with the standard curve to quantitatively obtain the concentration value of the polyoxyethylene nonyl phenyl ether. The invention has the advantages of simple pretreatment, easy operation, wide linear range, high enrichment factor, better reproducibility and accurate qualitative determination, has no need of adopting expensive liquid phase chromatography-mass spectrometry analyzers, and can quickly analyze the concentration of the polyoxyethylene nonyl phenyl ether in water.

The invention relates to the technical field of monitoring of floating oil, and in particular relates to thermal infrared imaging measuring system and method for an oil film on the water surface. The system comprises a thermal infrared imaging instrument, an optical filter unit, a polarizing film unit and a reflecting vibration mirror which are sequentially arranged; the thermal infrared imaging instrument is used for acquiring thermal infrared information of the current water source; the optical filter unit is equipped with a plurality of optical filters with different wave bands, and the plurality of optical filters can be adjusted to be respectively centered to a lens of the thermal infrared imaging instrument; the polarizing film unit is equipped with a plurality of polarizing films with different polarizing angles, and the plurality of polarizing films can be adjusted to be respectively centered to a lens of the thermal infrared imaging instrument; the reflecting vibration mirror is used for adjusting the working angle. Compared with the prior art, the system has the advantages that the interference of outside solar radiation, wind speed and other time and environmental factors on the measurement of the oil film on the water surface are avoided; the system is small in size, can quickly and accurately qualitatively and quantitatively measure the floating oil on the water surface, and is relatively high in operability, and controllable in manufacturing cost and use cost.

The invention provides a method for detecting effective ingredients of isodon lophanthoides. According to the detection method, the content of isoschaftoside, isoschaftoside and rosmarinic acid in isodon lophanthoides is determined by employing HPLC; and the conditions of HPLC comprise: a C18 reversed phase chromatographic column employing octadecylsilane bonded silica as a filling agent is employed, the mobile phase comprises a mobile phase A which is methanol, and a mobile phase B which is a 0.1% (volume) aqueous solution of phosphoric acid, and gradient elution is performed; the program of gradient elution comprises: 0-30 min, 30% of the mobile phase A and 70% of the mobile phase B; 30-70 min, 30% -> 50% of the mobile phase A, and 70% -> 50% of the mobile phase B, wherein the ratio of the mobile phases employs the volume percentage. The detection method successively helps to realize simultaneous determination on content of effective ingredients flavonoids and rosmarinic acid in isodon lophanthoides by employing HPLC, and the data can be used to characterize the medicinal material quality of isodon lophanthoides. A chromatogram of the content of isoschaftoside, isoschaftoside and rosmarinic acid is shown in the specification.

The invention discloses a vegetable oil pesticide residue detection method based on a three-dimensional fluorescence spectroscopic technology. The method comprises the steps that a fluorescence spectrometer is utilized to acquire an apparent spectrum of all vegetable oil pesticide residue samples, instrument excited emission correction is performed to obtain a real spectrum of the samples, and then pretreatment operation on spectral data is completed through a polynomial smoothing method; the number of optimal fluorescence components of the samples is determined by use of an APTLD model in combination with a nuclear consistent diagnosis method, a resolution spectrum of all the components is obtained, and the resolution spectrum is fitted with the real spectrum in a database to realize qualitative analysis of all the components of the samples; and a pesticide spectrum and a corresponding concentration are trained through a BP neural network algorithm to find a mapping relation, the resolution spectrum is input into the neural network completing training, and quantitative analysis of all the components of the to-be-detected samples is realized. Through the method, vegetable oil pesticide residue detection can be completed rapidly and accurately, and realization of a vegetable oil detection system which is easy to operate, fast in detection and portable is benefited.

The invention discloses a cigarette paper quality stability testing method, which comprises the following steps of (1) respectively shearing cigarette paper samples with the same specifications in different batches into fragments, and then performing cracking, wherein the cracking conditions are as follows: the initial temperature is 300+ / -20 DEG C, the temperature rise rate is 20+ / -5 DEG C / ms, and the cracking temperature is 900+ / -20 DEG C; (2) performing GC-MS (gas chromatography-mass spectrometer) analysis on cracking products, and obtaining the quality stability of the cigarette paper samples with the same specifications in different batches according to analysis results of aroma substances in the cracking products. The number of the cigarette paper samples with the same specifications in different batches is 3 to 5. The testing method has the advantages that the thermal cracking GC-MS is used; cigarette paper is subjected to heating cracking at a certain temperature in gas atmosphere; by analyzing the integral area change of ions extracted from aroma components in the cracking products of the cigarette paper with the same specifications in different batches, the test on the cigarette paper quality stability is realized.

The invention discloses a method for extracting a pure ethylene-ethylene vinyl-acetate copolymer. The ethylene-ethylene vinyl-acetate copolymer is purified through utilizing the difference between two substances in mobility in a mixture of the ethylene-ethylene vinyl-acetate copolymer and paraffin in a solvent.

The invention provides a detection test strip of salmonella typhimurium. The detection test strip comprises a sample pad, a cellulose nitrate membrane, absorbent paper and a bottom plate. A quantum dot fluorescent micro-sphere is marked with a monoclonal antibody of salmonella typhimurium, and the cellulose nitrate membrane is coated with a detection antibody and a quality control antibody. Basedon the test strip, the invention also provides a method for high-sensitivity and quick quantitative detection of salmonella typhimurium. The method fully utilizes super-strong and stable fluorescent signals of quantum dots, combines the advantages of easy marking and control of polymer sub-micro-spheres, constructs an immunochromatography technology of quantum dot fluorescent micro-spheres, and realizes sensitive and quick detection of salmonella typhimurium.

The invention relates to the technical field of lobster eye devices. The invention discloses a device and a method for detecting defects of a lobster eye optical device square hole array structure. The device comprises an X-ray light tube, an optical slit, a multi-axis displacement table, a detector and a data analysis system, wherein the lobster eye optical device is arranged between the X-ray light tube and the detector, the optical slit is arranged between the X-ray light tube and the lobster eye optical device, the lobster eye optical device is installed on the X-ray light tube, and the centers of the X-ray light tube, the lobster eye optical device, the optical slit and the detector are coaxial and correspondingly flush. By combining lobster eye optical focusing and a small hole X-rayimaging method, the square hole structure defects of different lobster eye optical devices, especially the deformation condition in a high-temperature preparation environment (hot bending), are tested so as to be beneficial to optimizing the focusing imaging quality of the lobster eye optical devices in the later period.

The invention discloses a plasma flow visualization device based on a glow discharge principle. The plasma flow visualization device comprises a plurality of electrode couples composed of anode electrodes and cathode electrodes, an insulating plate, a dividing wall, an amplifier, a variable voltage variable frequency device, a high-voltage probe, a modulation-demodulation device and a data acquisition device. Three-phase electricity provided by an external power supply is sequentially converted into high-frequency high-voltage alternating currents through the amplifier and the variable voltage variable frequency device, the high-frequency high-voltage alternating currents are loaded to the anode electrodes and the cathode electrodes of the electrode couples, plasma fields are generated between the anode electrodes and the cathode electrodes, high-frequency high voltage between the anode electrodes and the cathode electrodes is obtained through the high-voltage probe, the modulation-demodulation device is adopted to separate originally-applied high-frequency high voltage from an incoming flow unsteady pressure fluctuation, the unsteady pressure fluctuation is collected and analyzed through the data acquisition device at last, a real unsteady pressure fluctuation of a wall is restored, and displaying of real flow fields is achieved.

The invention discloses a method for visually detecting melamine through nanogold hydrosol without an aptamer. The method comprises the following steps: adding plants into absolute ethyl alcohol, boiling, carrying out extraction, and filtering, so as to obtain filtrate; adding activated carbon into the filtrate for decolorization, and standing for 1 day, so as to obtain a faint yellow plant extracting solution; diluting the faint yellow plant extracting solution, and refrigerating a obtained diluent for later use; and extracting the diluent, adding a to-be-tested sample into the diluent, uniformly mixing, adding a chloroauric acid water solution to adjust the pH, reacting, and detecting melamine by virtue of a visual colorimetric method or an ultraviolet visible spectrophotometry. The method is rapid, simple, convenient, high in sensitivity and strong in anti-jamming capability, and the detection limit to melamine can reach 15nM. The method can be used for visually detecting a melaminesample, the field sampling detection can be realized, the detection sensitivity is high, and the qualitative and quantitative detection of melamine can be realized; and the sample can be detected ina severe environment, so that the application range of the sample is wide.

The invention discloses a biochemical sensor based on a polymer long-period waveguide optical grating and a preparation method thereof. The biochemical sensor comprises a silicon substrate layer, a lower covering layer, a core layer and an upper covering layer which are overlapped sequentially from bottom to top, wherein the upper covering layer is provided with a liquid channel; and the core layer comprises a straight waveguide, and a first long-period optical grating and a second long-period optical grating which are connected with one side of the straight waveguide and stretch into the liquid channel. Furthermore, the preparation method adopts a mask plate I and a mask plate II, and combines a magnetron sputtering process, a photoetching process, a wet-process etching process, a dry-process etching process and the like, so that the biochemical sensor can be conveniently and rapidly prepared; and compared with a traditional biochemical sensor, the biochemical sensor disclosed by the invention has the advantages of small size, simple structure, high sensitivity, rapid detection, low cost, convenience for integration and detection diversity.

The invention relates to the field of molecular diagnosis, and especially relates to a fully-automatic real-time fluorescent quantitative PCR workstation. The fully-automatic real-time fluorescent quantitative PCR workstation comprises a frame, a three-axis clamping jaw module, a pipetting module, a 96-well plate station module, a uniform mixing module and a real-time fluorescent quantitative PCRmodule. The defects that traditional nucleic acid detection is low in automation degree, poor in sample processing capacity and the like are overcome, and fully-automatic nucleic acid qualitative or quantitative measurement can be achieved.