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Method for visually detecting melamine through nanogold hydrosol without aptamer

A technology of melamine and nano-gold, which is applied in the preparation of test samples, material analysis through observation of the influence on chemical indicators, and measurement devices, can solve problems such as low cost, heavy environmental pollution, and complicated procedures, and achieve cost reduction. Low, strong anti-interference ability, high detection flexibility

Active Publication Date: 2018-12-07
HAINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the deficiencies of low cost, large environmental pollution, and complicated procedures in current melamine detection methods, the present invention provides a method for the visual detection of melamine without aptamer nano-gold hydrosol. The method does not use aptamers and is easy to operate and Fast, short process, high sensitivity, environmentally friendly, capable of on-site random visual detection of melamine samples

Method used

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  • Method for visually detecting melamine through nanogold hydrosol without aptamer
  • Method for visually detecting melamine through nanogold hydrosol without aptamer
  • Method for visually detecting melamine through nanogold hydrosol without aptamer

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] 1. Green and rapid preparation of nano-gold hydrosol

[0046] 1.1. Extraction of the active ingredients of Baisha green tea: Weigh 4.0g of Baisha green tea dry powder pulverized through a 10-mesh sieve into a three-necked flask, add 160 mL of absolute ethanol to the flask, place the flask in a constant temperature magnetic stirrer, and set the temperature to 80 ℃, constant temperature boiling for 1 hour, cooled to room temperature, suction filtration to obtain the filtrate. 1.5 g of activated carbon was added to the filtrate, stirred for three hours for decolorization, and left for one day after filtration to obtain a pale yellow plant extract. Use a pipette to accurately measure 100 mL of the plant extract into a 500 mL volumetric flask, dilute to the mark with distilled water, and store the diluted extract and stock solution in a 4°C refrigerator for later use.

[0047] 1.2. Preparation of nano-gold hydrosol: Take 5 mL of the diluted Baisha green tea extract in a 20-...

Embodiment 2

[0077] Embodiment 2 The influence of pH and NaCl concentration on detection of melamine

[0078] 1. Green and fast preparation of plant extract

[0079]Extraction of the active ingredients of Baisha green tea: Weigh 4.0g of the dry powder of Baisha green tea pulverized through a 10-mesh sieve into a three-necked flask, add 160 mL of absolute ethanol to the flask, place the flask in a constant temperature magnetic stirrer, and set the temperature to 80°C , boiled at a constant temperature for 1 hour, cooled to room temperature, and suction filtered to obtain a filtrate. 1.5 g of activated carbon was added to the filtrate and stirred for three hours to decolorize, and after filtration, it was left for one day to obtain a pale yellow plant extract. Use a pipette to accurately measure 100 mL of the plant extract into a 500 mL volumetric flask, dilute to the mark with distilled water, and store the diluted extract and stock solution in a 4°C refrigerator for later use.

[0080] 2...

Embodiment 3

[0086] Example 3 Anti-interference experiment

[0087] In order to prove the detection accuracy and high selectivity of the method of the present invention, some other salts or reagents containing hydroxyl and amino groups (ascorbic acid, arginine, histidine, lysine, tryptophan, etc.) which may interfere with the detection results were selected at the same time. acid, glucose, urea, sodium chloride, calcium chloride and lactose) are detected by the method of the present invention, and the steps are:

[0088] Take 5mL of the diluted Baisha green tea extract, respectively, add melamine, ascorbic acid, arginine, histidine, lysine, tryptophan, glucose, urea, sodium chloride to each diluted Baisha green tea extract. , calcium chloride, lactose, the concentration of each reagent in the system is 0.1g / L, mix well at room temperature, then add 1mL of 1mM chloroauric acid aqueous solution, adjust the pH to 6.5, and react at room temperature until the color of the solution no longer cha...

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Abstract

The invention discloses a method for visually detecting melamine through nanogold hydrosol without an aptamer. The method comprises the following steps: adding plants into absolute ethyl alcohol, boiling, carrying out extraction, and filtering, so as to obtain filtrate; adding activated carbon into the filtrate for decolorization, and standing for 1 day, so as to obtain a faint yellow plant extracting solution; diluting the faint yellow plant extracting solution, and refrigerating a obtained diluent for later use; and extracting the diluent, adding a to-be-tested sample into the diluent, uniformly mixing, adding a chloroauric acid water solution to adjust the pH, reacting, and detecting melamine by virtue of a visual colorimetric method or an ultraviolet visible spectrophotometry. The method is rapid, simple, convenient, high in sensitivity and strong in anti-jamming capability, and the detection limit to melamine can reach 15nM. The method can be used for visually detecting a melaminesample, the field sampling detection can be realized, the detection sensitivity is high, and the qualitative and quantitative detection of melamine can be realized; and the sample can be detected ina severe environment, so that the application range of the sample is wide.

Description

technical field [0001] The invention relates to a method for detecting melamine, in particular to a method for visually detecting melamine with low cost and no aptamer nano-gold hydrosol, and relates to the technical field of melamine detection. technical background [0002] Melamine is a white, odorless, nitrogen-containing heterocyclic compound with the molecular formula C 3 N 3 (NH 2 ) 3 , is widely used in chemical industries such as plastics, fertilizers and resins, it is harmful to human body and cannot be used for food processing or food addition. However, since melamine has a high nitrogen content and is cheap, adding it as a protein-rich raw material to food can significantly increase the total protein content, so some unscrupulous merchants add melamine to milk, feed, etc. Melamine can be hydrolyzed into cyanuric acid in the human body, and the two combine to form a network of insoluble matter. Excessive accumulation of the insoluble matter in the body will cau...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N21/31G01N1/38
CPCG01N1/38G01N21/3103G01N21/78
Inventor 付云芝李彦景周美双李琼霞杨晓红汪国庆
Owner HAINAN UNIVERSITY
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