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35results about How to "Inhibition of non-specific adsorption" patented technology
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Biochip sensor surface carrying polyethylene glycolated nanoparticles
InactiveCN1646912AInhibition of non-specific adsorptionMaterial nanotechnologyCatalyst regeneration/reactivationSensor materialsBiochip
The invention provides high sensitivity bioassay sensor systems in which non-specific adsorption of impurities such as, for example, proteins, in biological samples is inhibited. Polyethylene glycolated particles enclosing metal or semi-conductor which is in common with the sensor material are used for amplification.
Owner:JAPAN SCI & TECH CORP
Application of hyperbranched polyglycerol modified magnetic nanoparticle microspheres in chemiluminescence immune assay
ActiveCN104316679AEasy to detectLowest detection limit optimizationMaterial analysisAntigenMicrosphere
The invention discloses application of hyperbranched polyglycerol modified magnetic nanoparticle microspheres in chemiluminescence immune assay. By utilizing the hyperbranched polyglycerol modified magnetic nanoparticle microspheres, a nanometer microsphere material of which the surface is hydrophilic and which is enriched in functional groups is prepared. When a chemiluminescence diagnostic agent based on the hyperbranched polyglycerol modified magnetic nanoparticle microspheres is actually used, the reagent can well inhibit nonspecific adsorption of proteins, and the antibody coupling amount is 2-3 times higher than that of commercial magnetic microspheres. In the project-based performance analysis, the diagnostic agent based on the magnetic microspheres can greatly enhance the reaction between antigens and antibodies. When the reagent is compared with a commercial magnetic microsphere reagent, the sensitivity is improved by 4-5 times, and the upper detection limit is improved by 1.3 times, so that the detection range is improved by nearly 6.5 times.
Owner:GETEIN BIOTECH
Method for performing hydrophilic modification on polyacrylate or copolymer thereof, and product and application of polyacrylate or copolymer thereof
ActiveCN102690380AImprove hydrophilicityInhibition of non-specific adsorptionIon-exchange process apparatusOther chemical processesChromatographic separationOrganic solvent
The invention discloses a method for performing hydrophilic modification on the surface of polyacrylate or a copolymer material thereof. The method comprises the following steps of: 1) performing swelling on polyacrylate or the copolymer material thereof by using an organic solvent; 2) chemically bonding hydrophilic polyamine substance onto the surface of the material treated in the step 1) in the presence of the solvent by covalent bonds; 3) crosslinking and reinforcing the hydrophilic polyamine substance on the surface of the material, which is obtained in the step 2); and 4) performing quaterisation on the hydrophilic substance on the surface of the material, which is obtained in the step 3), in the presence of the solvent by using quaternary ammonium salt biochemical reagent. The method can be applied to the surface modification of most polymer materials, particularly superporous polymer microspheres, and the polymer microspheres which are subjected to hydrophilic modification can be applied to the field of biotechnologies and chromatographic separation.
Owner:INST OF PROCESS ENG CHINESE ACAD OF SCI
Three-dimensional carrier of microarray biochip and preparation method thereof
InactiveCN103147133ALarge specific surface areaIncrease surface densityPeptide librariesNucleotide librariesDiseaseZinc oxide nanorod
The invention discloses a three-dimensional carrier of a microarray biochip and a preparation method thereof. The three-dimensional carrier of a microarray biochip is prepared by growing a zinc oxide nanorod on the surface of a substrate material and growing a polymer brush on the surface of the zinc oxide nanorod. The preparation method comprises the following steps of: activating the surface of the substrate material, and growing a zinc oxide nanorod on the surface of the substrate material; performing surface modification on the grown zinc oxide nanorod; connecting the modified surface of the zinc oxide nanorod with an initiator; and finally, growing a polymer brush to obtain a three-dimensional carrier of a microarray biochip. The preparation method is simple, does not need special equipment, has low production cost and good repeatability, and can be used for realizing large-scale production; the prepared three-dimensional carrier has low price and high fixing density for probe molecule, can be used for effectively inhibiting non-specific protein adsorption and improving the sensitivity and the specificity, and can be widely applied to the fields of biology, basic medicine, disease diagnosis, drug screening, food safety, environmental monitoring and the like.
Owner:SOUTHWEST UNIV
Bilayer lipid membrane surface modified protein chip and its manufacturing method and use
ActiveCN101038290AHigh selectivityPrevent non-specific adsorptionBiological testingLipid formationProtein insertion
The present invention relates to a bilayer lipids membrane surface modified protein chip, a silicon substrate being a solid substrate on which being, in turn, a L-alpha-phosphatidylethanolamine monomolecular layer linked to the silicon substrate through covalent bonds and a p-nitrophenyl ester-polyethylene glycol-(1,2-dioleoyl-3-glycerophosphatide ethanolamine) layer associated with the phosphatidylethanolamine monomolecular layer by a hydrophobic effect. The protein chip is obtained by fixing the L-alpha-phosphatidylethanolamine monomolecular layer at the surface of the silicon substrate through covalent bonds in a micro- runner system and then adsorbing the p-nitrophenyl ester-polyethylene glycol-(1,2-dioleoyl-3-glycerophosphatide ethanolamine) layer. Said protein chip is capable of applied in an imaging ellipsometry biosensor to explore target molecules capable of associating specifically therewith by covalently fixing ligands molecules at terminal tetrafunctional radicals of the protein chip surface PEG, wherein the surface morphology changes of the protein chip are capable of being observed in said imaging ellipsometry system, after both are associated. The bilayer lipids membrane surface modified protein chip in accordance with the present invention is capable of avoiding effectively a nonspecific adsorption, with its ligands molecules having excellent stability and being kept high biological activities.
Owner:INST OF MECHANICS - CHINESE ACAD OF SCI
Preparation method of sensor electrode surface anti-biological-pollution coating
ActiveCN107976472ANon-immunogenicGood biocompatibilityMaterial electrochemical variablesEnvironmental resistanceFood safety
The invention discloses a preparation method of a sensor electrode surface anti-biological-pollution coating. The method is characterized by comprising two major steps of synthesizing photosensitive hyaluronic acid and preparing an anti-biological-pollution coating. The prepared photosensitive hyaluronic acid has high hydrophilcity, optical crosslinking performance and good biocompatibility. The synthesis of the photosensitive hyaluronic acid is simple; the raw materials can be easily obtained; the industrial production can be easily realized; through the introduction of photosensitive monomers, on the basis that the coating achieves the self excellent characteristics of the hyaluronic acid, the coating structure can be fixed through ultraviolet light crosslinking. When the photosensitivehyaluronic acid is applied to raw liquid sample detection, a sensor electrode based on modification by the photosensitive hyaluronic acid coating has long-term and efficient anti-biological-pollutioncapability. The light crosslinking technology, the functional coating technology and the biosensing technology are combined; the preparation method can be widely applied to the fields of food safety,biological medicine, environment protection monitoring, life safety and the like.
Owner:JIANGNAN UNIV
Surface zwitterionic polymer modified magnetic microsphere as well as preparation method and application of magnetic microsphere
ActiveCN113351182AImprove hydrophilicityGuaranteed Magnetic ResponsivenessOther chemical processesAlkali metal oxides/hydroxidesBiological macromoleculeActive agent
The invention relates to a surface zwitterionic polymer modified magnetic microsphere and a preparation method and application thereof, and the preparation method comprises the following steps: (1) preparing magnetic nanoparticles, and dispersing the magnetic nanoparticles in an organic solvent to prepare a magnetic fluid; (2) dispersing the magnetic fluid into a water phase containing a surfactant, and preparing submicron magnetic microspheres by using a reverse microemulsion method; (3) preparing silica modified magnetic nanoparticles; and (4) dispersing in a solvent containing an anionic surfactant, a carboxyl monomer, an amino monomer, a cross-linking agent and an initiator, heating, reacting and polymerizing to obtain the superparamagnetic microspheres coated with the zwitterionic polymer on the surface. The active groups on the surface of the magnetic microsphere can be controlled through the proportion of monomers, biological macromolecules such as nucleic acid and protein can be efficiently and specifically coupled, non-specific binding of the biological macromolecules on the surface of the magnetic microsphere can be inhibited, and the sensitivity and cell sorting efficiency in protein detection are greatly improved.
Owner:SHANGHAI JIAO TONG UNIV
Liquid chromatography column, and method for analyzing hemoglobin
ActiveCN103443622AReduce productionImprove economyComponent separationBiological bodyPolymethyl methacrylate
A purpose of the present invention is to provide a liquid chromatography column which can be produced at a low cost and rarely undergoes the non-specific adsorption of a biological component thereon. In addition, the purpose of the invention is to provide a method for analyzing hemoglobin by using the liquid chromatography column. The present invention is a liquid chromatography column comprising a column pipe and an end fitting, wherein the column pipe and the end fitting are made of polyethylene or polymethyl methacrylate.
Owner:SEKISUI MEDICAL CO LTD
Confining liquid and confining method of biochip for detecting various proteins
InactiveCN102914654AGood chemical stabilityElimination of associativityBiological testingFreeze-dryingPhosphate
The invention discloses a confining liquid of a biochip for detecting various proteins. The confining liquid is a polyvinylidene fluoride confining liquid. The confining liquid is 50-100 percent solution obtained by diluting phosphate buffer. The invention provides a confining method of the biochip for detecting various proteins. The confining method comprises the following steps of: firstly, adding a primary antibody and adding the confining liquid after reaction; secondly, carrying out cryopreservation on the mixed solution at the temperature of 40DEG C below zero for two hours; and thirdly, carrying out freeze drying on the mixture at the temperature 80DEG C below zero in a vacuum state. According to the confining liquid and the confining method disclosed by the invention, the polyvinylidene fluoride confining liquid is used as the confining liquid in the biochip for detecting various proteins; and when the biochip is used for detecting various proteins, the non-specific free combination and cross reaction can be eliminated and false positive is avoided.
Owner:董方
Superposition type fixed bed phase-conversion focusing chromatographic column and separation method
InactiveCN102302866BAchieve focusReduced separation timeIon-exchange process apparatusHydrolasesDismutaseFixed bed
The invention provides a superposition type fixed bed phase-conversion focusing chromatographic column and further provides a method which simultaneously separates lumbrukinase, superoxide dismutase and compound amino acid by adopting the same. The present status that single finished products are produced in the traditional biological pulp production line is reformed, and intensified continuous separation for a plurality of drug effect ingredients in the same column bed is realized under the premise that the economic accounting indicators, such as quality, yield, energy consumption, working hours and the like of the original biochemical products are kept at the original level.
Owner:北京双螺旋生物技术研究所
Polydimethylsiloxane surface modification method based on hydrophobin/methylcellulose
InactiveCN103772728AImprove coating stabilityImprove surface hydrophilicityAntifouling/underwater paintsPaints with biocidesMethyl cellulosePolydimethylsiloxane
The invention discloses a polydimethylsiloxane surface modification method based on hydrophobin / methylcellulose. The hydrophobin SC3 and the methylcellulose are used as the surface modifier to be physically adsorbed onto the surface of a micropassage of the polydimethylsiloxane to form an amphipathic monomolecular film, then a chip passage is heated, so that the structure conversion of the hydrophobin SC3 can be induced by the methylcellulose, and a stable amphipathic monomolecular film is formed on the surface of the micropassage of the polydimethylsiloxane. By adopting the method, the coating stability of the polydimethylsiloxane can be improved, the surface hydrophily of the passage can be improved, the non-specificity adsorption of the protein on the surface of the micropassage can be effectively prevented, the antipollution capacity of the surface of the polydimethylsiloxane can be improved, and the high-efficiency reproducibility and separation of an analyzed substance can be realized.
Owner:SHAANXI NORMAL UNIV
Method for preparing dendritic-polymer-based microarray antibody chip and product thereof
ActiveCN104034884AEasy to prepareHighly selective detectionMaterial analysisChemistryDichloromethane
The invention discloses a method for preparing a dendritic-polymer-based microarray antibody chip and a product of the method. The method for preparing the dendritic-polymer-based microarray antibody chip comprises the steps that a glass slide is cleaned in ethyl alcohol, and then is immersed in an ethanol solution containing 3-glycidoxypropyltrimethoxysilane, and after annealing, the glass slide is immersed into a dry dichloromethane or tetrahydrofuran solution containing triethylamine and alpha-bromoisobutyryl bromide, and then is flushed with dichloromethane or tetrahydrofuran; nitrogen is blown into a methanol solution containing oligo (ethylene glycol) methacrylate and glycidyl methacrylate, 2,2'-bipyridyl and cuprous bromide are added into the methanol solution under ultrasonic waves, the glass slide is immersed into the methanol solution, and the methanol solution is sealed and stored in an inert and light-avoiding atmosphere box to obtain an activated carrier material; finally, a microarray chip sample application instrument is used for printing detection antibodies on the activated carrier material, standing is carried out, and the microarray antibody chip is obtained through washing with a phosphate buffer solution. The obtained microarray antibody chip is high in sensitivity, good in specificity and capable of being used for early stage screening of diseases.
Owner:SOUTHWEST UNIVERSITY
Method for inhibiting non-specific adsorption of solid material surface
InactiveCN101745495BEasy to manufactureEfficient manufacturingBiological testingSpecial surfacesHigh volume manufacturingSurface-active agents
The invention relates to a method for inhibiting non-specific adsorption of a solid material surface, in particular to a method for solving the problem of non-specific adsorption of large biological molecule, germ, cell and the like through combining the non-ionic surface active agent on fluoride monomolecular layer surface or nylation monomolecular layer surface. The invention comprises: a layerof fluoride monomolecular layer film or nylation monomolecular layer film is modified on the solid material surface, and when using mixed non-ionic surface active agent to prepare buffer solution containing large biological molecule, the large biological molecule can inhibit non-specific adsorption of large biological molecule in the buffer solution together with the mixed non-ionic surface active agent and the fluoride monomolecular layer film or nylation monomolecular layer film. Through the cooperation of non-ionic surface active agent, and fluoride surface or nylation surface, the invention can simply, effectively and fast prepare the large biological molecule microarray detection part, is easy to operate, has low cost and is applicable for mass production.
Owner:TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
Method for modifying polydimethylsiloxane by hydrophobic coating self-assembly by using ion complementary peptide
InactiveCN104497334AImprove adsorption capacityInhibition of non-specific adsorptionLaboratory glasswaresCoatingsAnalyteDodecyl-beta-D-maltoside
The invention discloses a method for modifying polydimethylsiloxane by hydrophobic coating self-assembly by using an ion complementary peptide, which comprises the following steps: by using an ion complementary peptide EAR16-II or ion complementary peptide EAR16-II, methyl cellulose and dodecyl-beta-D-maltoside as a surface modifier, carrying out physical adsorption to perform self-assembly on the polydimethylsiloxane microchannel surface so as to form a stable hydrophobic film; and in the presence of saccharide derivatives, changing the configuration of the EAR16-II to form the more stable beta-folding self-assembled hydrophobic film. The method can effectively inhibit the non-specific adsorption of the protein to the microchannel surface, enhances the surface anti-pollution capacity and biological compatibility of the polydimethylsiloxane, and implements high-efficiency recurrent separation of the analyte.
Owner:SHAANXI NORMAL UNIV
Gold magnetic nanoprobe based on ordered arrangement of aptamers and application of gold magnetic nanoprobe in okadaic acid detection
PendingCN114657185AAchieve saturated coverageInhibition of non-specific adsorptionMaterial analysis by electric/magnetic meansBiological testingAptamerHigh density
The invention discloses a gold magnetic nanoprobe based on ordered arrangement of aptamers and application of the gold magnetic nanoprobe in okadaic acid detection (OA). The gold magnetic nanoprobe is formed by combining nanogold spherical nucleic acid and nano magnetic beads, wherein nucleic acid sequences are orderly arranged on the surface of the nanogold spherical nucleic acid; the nucleic acid sequence is formed by an aptamer with an adenine base polymeric chain at the 5'end and a DNA complementary chain with a fluorescent label at the 5 'end. The surface of the gold magnetic nanoprobe is modified with high density and is loaded with orderly arranged aptamers, so that the gold magnetic nanoprobe is high in specific recognition efficiency and simple and convenient to separate a target object, and can react with okadaic acid to release a fluorescently-labeled DNA complementary chain, and laser-induced fluorescence detection is applied, so that high-sensitivity analysis of trace OA is realized.
Owner:FUZHOU UNIV
Three-dimensional carrier of microarray biochip and preparation method thereof
InactiveCN103147133BLarge specific surface areaIncrease surface densityPeptide librariesNucleotide librariesDiseaseZinc oxide nanorod
The invention discloses a three-dimensional carrier of a microarray biochip and a preparation method thereof. The three-dimensional carrier of a microarray biochip is prepared by growing a zinc oxide nanorod on the surface of a substrate material and growing a polymer brush on the surface of the zinc oxide nanorod. The preparation method comprises the following steps of: activating the surface of the substrate material, and growing a zinc oxide nanorod on the surface of the substrate material; performing surface modification on the grown zinc oxide nanorod; connecting the modified surface of the zinc oxide nanorod with an initiator; and finally, growing a polymer brush to obtain a three-dimensional carrier of a microarray biochip. The preparation method is simple, does not need special equipment, has low production cost and good repeatability, and can be used for realizing large-scale production; the prepared three-dimensional carrier has low price and high fixing density for probe molecule, can be used for effectively inhibiting non-specific protein adsorption and improving the sensitivity and the specificity, and can be widely applied to the fields of biology, basic medicine, disease diagnosis, drug screening, food safety, environmental monitoring and the like.
Owner:SOUTHWEST UNIV
Method for modifing protein chip surface
ActiveCN1598588AImprove featuresMaintain biological activityMicrobiological testing/measurementBiological testingProtein moleculesProtein insertion
The invention discloses a method to modify proteid chip surface, its character is fixing a layer of low molecular weight proteid molecule on the chip by normal method at first, then fixing the proteid chip which is needed to be fixed. The low molecular weight is 1X10 to the power 3 -9X10 to the power 5 Dalton. The method can modify the surface character of the proteid chip surface: on one hand, because of the covering of the low molecular weight molecule on the chip surface, the biology activity of the unfixed proteid molecule can be ensured, and at the same time, the aspecfic absorb is controlled on the surface; on the other hand, the low molecular weight molecule has amino, carboxyl, hydroxyl and other active group polar aminophenol on the proteid molecule surface, they can be used to fix porteid molecule directly after being activeted, and to form stable covalent bond.
Owner:INST OF MECHANICS - CHINESE ACAD OF SCI
Method for performing hydrophilic modification on polyacrylate or copolymer thereof, and product and application of polyacrylate or copolymer thereof
ActiveCN102690380BImprove hydrophilicityInhibition of non-specific adsorptionIon-exchange process apparatusOther chemical processesChromatographic separationMicrosphere
The invention discloses a method for performing hydrophilic modification on the surface of polyacrylate or a copolymer material thereof. The method comprises the following steps of: 1) performing swelling on polyacrylate or the copolymer material thereof by using an organic solvent; 2) chemically bonding hydrophilic polyamine substance onto the surface of the material treated in the step 1) in the presence of the solvent by covalent bonds; 3) crosslinking and reinforcing the hydrophilic polyamine substance on the surface of the material, which is obtained in the step 2); and 4) performing quaterisation on the hydrophilic substance on the surface of the material, which is obtained in the step 3), in the presence of the solvent by using quaternary ammonium salt biochemical reagent. The method can be applied to the surface modification of most polymer materials, particularly superporous polymer microspheres, and the polymer microspheres which are subjected to hydrophilic modification can be applied to the field of biotechnologies and chromatographic separation.
Owner:INST OF PROCESS ENG CHINESE ACAD OF SCI
Bilayer lipid membrane surface modified protein chip and its manufacturing method and use
ActiveCN101038290BHigh selectivityPrevent non-specific adsorptionBiological testingPolyethylene glycolAcyl group
The present invention relates to a bilayer lipids membrane surface modified protein chip, a silicon substrate being a solid substrate on which being, in turn, a L-alpha-phosphatidylethanolamine monomolecular layer linked to the silicon substrate through covalent bonds and a p-nitrophenyl ester-polyethylene glycol-(1,2-dioleoyl-3-glycerophosphatide ethanolamine) layer associated with the phosphatidylethanolamine monomolecular layer by a hydrophobic effect. The protein chip is obtained by fixing the L-alpha-phosphatidylethanolamine monomolecular layer at the surface of the silicon substrate through covalent bonds in a micro- runner system and then adsorbing the p-nitrophenyl ester-polyethylene glycol-(1,2-dioleoyl-3-glycerophosphatide ethanolamine) layer. Said protein chip is capable of applied in an imaging ellipsometry biosensor to explore target molecules capable of associating specifically therewith by covalently fixing ligands molecules at terminal tetrafunctional radicals of the protein chip surface PEG, wherein the surface morphology changes of the protein chip are capable of being observed in said imaging ellipsometry system, after both are associated. The bilayer lipids membrane surface modified protein chip in accordance with the present invention is capable of avoiding effectively a nonspecific adsorption, with its ligands molecules having excellent stability and being kept high biological activities.
Owner:INST OF MECHANICS CHINESE ACAD OF SCI
Application of hyperbranched polyglycidyl ether modified nano-magnetic microspheres in chemiluminescence immunoassay
ActiveCN104316679BEasy to detectLowest detection limit optimizationMaterial analysisAntigenMicrosphere
The invention discloses application of hyperbranched polyglycerol modified magnetic nanoparticle microspheres in chemiluminescence immune assay. By utilizing the hyperbranched polyglycerol modified magnetic nanoparticle microspheres, a nanometer microsphere material of which the surface is hydrophilic and which is enriched in functional groups is prepared. When a chemiluminescence diagnostic agent based on the hyperbranched polyglycerol modified magnetic nanoparticle microspheres is actually used, the reagent can well inhibit nonspecific adsorption of proteins, and the antibody coupling amount is 2-3 times higher than that of commercial magnetic microspheres. In the project-based performance analysis, the diagnostic agent based on the magnetic microspheres can greatly enhance the reaction between antigens and antibodies. When the reagent is compared with a commercial magnetic microsphere reagent, the sensitivity is improved by 4-5 times, and the upper detection limit is improved by 1.3 times, so that the detection range is improved by nearly 6.5 times.
Owner:GETEIN BIOTECH
Surface modification method for QCM wafer
ActiveCN109557297AHigh sensitivityImprove accuracyBiological testingMaterial weighingAntibody antigen reactionsUltraviolet lights
The invention relates to a surface modification method for a QCM wafer. The QCM wafer with the gold-plated surface is soaked with an ethanol solution of ethyl 3-mercaptopropionate, ethanol is used forcleaning the surface, and the surface is naturally air-dried; the treated QCM wafer is soaked with a photoactive polymer solution with an active ester group, a strip-shaped or grid-shaped mask is used for covering the surface of the wafer, and a polymer is bonded with the surface of the wafer under the induction of ultraviolet light to form an array structure; absolute ethanol and deionized waterare used for sequentially cleaning the surface of the wafer to remove the unreacted bonded polymer; and the wafer is soaked with a buffer solution containing an antibody, the wafer is cleaned by using the deionized water, and the wafer is stored in the buffer solution for later use. The method has the advantages that a polymer film with a certain pattern structure is prepared on the surface of the QCM wafer through a light-induced grafting technology, and antibody molecules are further grafted to the surface of the film to form an ''antenna'' array capable of capturing specific biomolecules through an antibody-antigen reaction, so that the sensitivity is high.
Owner:南通市产品质量监督检验所
Preparation method and product of dendrimer-based microarray antibody chip
Owner:SOUTHWEST UNIV
Surface modification method of polydimethylsiloxane based on hydrophobin/methylcellulose
InactiveCN103772728BImprove coating stabilityImprove surface hydrophilicityAntifouling/underwater paintsPaints with biocidesMethyl celluloseNon specificity
The invention discloses a polydimethylsiloxane surface modification method based on hydrophobin / methylcellulose. The hydrophobin SC3 and the methylcellulose are used as the surface modifier to be physically adsorbed onto the surface of a micropassage of the polydimethylsiloxane to form an amphipathic monomolecular film, then a chip passage is heated, so that the structure conversion of the hydrophobin SC3 can be induced by the methylcellulose, and a stable amphipathic monomolecular film is formed on the surface of the micropassage of the polydimethylsiloxane. By adopting the method, the coating stability of the polydimethylsiloxane can be improved, the surface hydrophily of the passage can be improved, the non-specificity adsorption of the protein on the surface of the micropassage can be effectively prevented, the antipollution capacity of the surface of the polydimethylsiloxane can be improved, and the high-efficiency reproducibility and separation of an analyzed substance can be realized.
Owner:SHAANXI NORMAL UNIV
Biodetector based on interference effect of ordered porous nanostructured thin film and method for detecting biomolecules
ActiveCN108344714BImprove signal-to-noise ratioHigh sensitivityNanoparticle analysisWithdrawing sample devicesBio moleculesNanostructure
The invention discloses a biological detector based on the interference effect of an ordered porous nanostructure film. The biological detector includes a detection pool, a sensing unit arranged in the detection pool, and an optical fiber spectrometer for detecting the reflection interference spectrum of the sensing unit. The sensing unit is a porous film with three-dimensional ordered nanopores, and the diameter of the nanopores is 20nm-500nm. The invention also discloses a method for detecting biomolecules by using the biodetector. When the substance to be measured is adsorbed on the pore surface of the ordered porous film as the sensing unit, it will change the spectral distribution of the reflected interference light, resulting in a spectral line shift, which can be used to quantitatively or qualitatively analyze the sample The concentration of substances in the medium, the attachment rate, the interaction, the change of the geometric size, etc.
Owner:SOUTHEAST UNIV
A kind of surface modification method of qcm wafer
ActiveCN109557297BHigh sensitivityImprove accuracyBiological testingMaterial weighingChemical solutionAntibody antigen reactions
The invention relates to a method for modifying the surface of a QCM wafer. The gold-plated QCM wafer is soaked with an ethanol solution of ethyl 3-mercaptopropionate, the surface is cleaned with ethanol and air-dried naturally; The treated QCM wafer is infiltrated with the substance solution, and the wafer surface is covered with a strip or grid mask. Under the induction of ultraviolet light, the polymer is bonded to the wafer surface to form an array structure; it is washed successively with absolute ethanol and deionized water. On the surface of the wafer, remove the unreacted bonded polymer; use the buffer solution containing the antibody to soak the wafer, use deionized water to clean the wafer, and store the wafer in the buffer solution for future use. The advantage of the present invention is that: a polymer film with a certain pattern structure is prepared on the surface of the QCM wafer by light-induced grafting technology, and antibody molecules are further grafted on the surface of the film to form a "antibody-antigen reaction" that can capture specific biomolecules. Antenna" array with high sensitivity.
Owner:南通市产品质量监督检验所
PH-responsive siRNA delivery system
PendingCN114748640AClear structureProcess stabilityOrganic active ingredientsGenetic material ingredientsTumor targetPropanoic acid
The invention relates to the technical field of biology, in particular to a pH responsive siRNA delivery system for a tumor growth factor receptor. The delivery system provided by the invention comprises a molecule formed by a positive-sense strand ssRNA modified by a polypeptide derivative and an antisense strand asRNA modified by a PEG derivative, polypeptide in the delivery system is c (RGDfK), PEG derivative modified antisense strand asRNA is formed by connecting and reacting methoxy polyethylene glycol propionaldehyde (mPEG-CHO) molecules, coupling molecules and asRNA, the PEG molecular weight in the mPEG-CHO is 500-20000, hydrazone bonds are formed between the mPEG-CHO and the coupling molecules, the coupling molecules comprise 8-amino-3, 5-dimethoxy-3, 5-dimethoxy-3, 5-dimethoxy-3, 5-dimethoxy-3, 5-dimethoxy-3, 5-dimethoxy-3, 5-dimethoxy-3, 5-dimethoxy-3, 5-dimethoxy-3, 5-dimethoxy-3, 5-dimethoxy-3, 5-dimethoxy-3, 5-dimethoxy-3, 5-dimethoxy-3, 5-dimethoxy-3, 5-dimethoxy-3, the organic solvent is one or more of 2, 6-dioxaoctanoic acid, 4-(benzyl amino) benzoyl hydrazine, N-maleyl-beta-alanine and 3-maleimidopropionic acid. The delivery system can prolong the in-vivo circulation time of the siRNA, inhibit metabolic accumulation of the siRNA in the kidney and improve the tumor targeted delivery efficiency, thereby better achieving the silence effect of the siRNA.
Owner:ZHUJIANG HOSPITAL SOUTHERN MEDICAL UNIV
Cellulose 6-aminocaproate and its synthesis method and application
The invention discloses 6-amino caproic acid cellulose ester as well as a synthetic method and application thereof. According to the 6-amino caproic acid cellulose ester, some hydroxyls of cellulose are substituted by a group as shown in the specification, and the total average degree of substitution of the group on each glucose unit ring is 0.6-1.2. The 6-amino caproic acid cellulose ester is synthesized from raw materials derived from a wealth of sources, the reaction conditions are gentle, the cost is low, the synthesized 6-amino caproic acid cellulose ester can be used as a surface modifying reagent for a polydimethylsiloxane micro-fluidic chip and physically adsorbed on the surface of a passage of the polydimethylsiloxane micro-fluidic chip to form a monomolecular film with positive charges, so that the surface hydrophilicity of the passage of the polydimethylsiloxane micro-fluidic chip can be improved, and nonspecific adsorption of protein on the surface of the passage of the micro-fluidic chip can be effectively inhibited, so high-efficiency reappearance and separation of analyte are realized.
Owner:SHAANXI NORMAL UNIV
Method for modifing protein chip surface
InactiveCN1320361CImprove featuresMaintain biological activityMicrobiological testing/measurementBiological testingProtein moleculesProtein insertion
The invention discloses a method to modify proteid chip surface, its character is fixing a layer of low molecular weight proteid molecule on the chip by normal method at first, then fixing the proteid chip which is needed to be fixed. The low molecular weight is 1X10 to the power 3 -9X10 to the power 5 Dalton. The method can modify the surface character of the proteid chip surface: on one hand, because of the covering of the low molecular weight molecule on the chip surface, the biology activity of the unfixed proteid molecule can be ensured, and at the same time, the aspecfic absorb is controlled on the surface; on the other hand, the low molecular weight molecule has amino, carboxyl, hydroxyl and other active group polar aminophenol on the proteid molecule surface, they can be used to fix porteid molecule directly after being activeted, and to form stable covalent bond.
Owner:INST OF MECHANICS CHINESE ACAD OF SCI
Superposition type fixed bed phase-conversion focusing chromatographic column and separation method
InactiveCN102302866AImprove ergonomicsReduced separation timeIon-exchange process apparatusHydrolasesChemistryChromatography column
The invention provides a superposition type fixed bed phase-conversion focusing chromatographic column and further provides a method which simultaneously separates lumbrukinase, superoxide dismutase and compound amino acid by adopting the same. The present status that single finished products are produced in the traditional biological pulp production line is reformed, and intensified continuous separation for a plurality of drug effect ingredients in the same column bed is realized under the premise that the economic accounting indicators, such as quality, yield, energy consumption, working hours and the like of the original biochemical products are kept at the original level.
Owner:北京双螺旋生物技术研究所
Phosphoryl choline chitosan derivative synthesis method
Owner:JINAN UNIVERSITY
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