35results about How to "Inhibition of non-specific adsorption" patented technology

The invention provides high sensitivity bioassay sensor systems in which non-specific adsorption of impurities such as, for example, proteins, in biological samples is inhibited. Polyethylene glycolated particles enclosing metal or semi-conductor which is in common with the sensor material are used for amplification.

The invention provides a superposition type fixed bed phase-conversion focusing chromatographic column and further provides a method which simultaneously separates lumbrukinase, superoxide dismutase and compound amino acid by adopting the same. The present status that single finished products are produced in the traditional biological pulp production line is reformed, and intensified continuous separation for a plurality of drug effect ingredients in the same column bed is realized under the premise that the economic accounting indicators, such as quality, yield, energy consumption, working hours and the like of the original biochemical products are kept at the original level.

The invention discloses a three-dimensional carrier of a microarray biochip and a preparation method thereof. The three-dimensional carrier of a microarray biochip is prepared by growing a zinc oxide nanorod on the surface of a substrate material and growing a polymer brush on the surface of the zinc oxide nanorod. The preparation method comprises the following steps of: activating the surface of the substrate material, and growing a zinc oxide nanorod on the surface of the substrate material; performing surface modification on the grown zinc oxide nanorod; connecting the modified surface of the zinc oxide nanorod with an initiator; and finally, growing a polymer brush to obtain a three-dimensional carrier of a microarray biochip. The preparation method is simple, does not need special equipment, has low production cost and good repeatability, and can be used for realizing large-scale production; the prepared three-dimensional carrier has low price and high fixing density for probe molecule, can be used for effectively inhibiting non-specific protein adsorption and improving the sensitivity and the specificity, and can be widely applied to the fields of biology, basic medicine, disease diagnosis, drug screening, food safety, environmental monitoring and the like.

The invention discloses a method for performing hydrophilic modification on the surface of polyacrylate or a copolymer material thereof. The method comprises the following steps of: 1) performing swelling on polyacrylate or the copolymer material thereof by using an organic solvent; 2) chemically bonding hydrophilic polyamine substance onto the surface of the material treated in the step 1) in the presence of the solvent by covalent bonds; 3) crosslinking and reinforcing the hydrophilic polyamine substance on the surface of the material, which is obtained in the step 2); and 4) performing quaterisation on the hydrophilic substance on the surface of the material, which is obtained in the step 3), in the presence of the solvent by using quaternary ammonium salt biochemical reagent. The method can be applied to the surface modification of most polymer materials, particularly superporous polymer microspheres, and the polymer microspheres which are subjected to hydrophilic modification can be applied to the field of biotechnologies and chromatographic separation.

The present invention relates to a bilayer lipids membrane surface modified protein chip, a silicon substrate being a solid substrate on which being, in turn, a L-alpha-phosphatidylethanolamine monomolecular layer linked to the silicon substrate through covalent bonds and a p-nitrophenyl ester-polyethylene glycol-(1,2-dioleoyl-3-glycerophosphatide ethanolamine) layer associated with the phosphatidylethanolamine monomolecular layer by a hydrophobic effect. The protein chip is obtained by fixing the L-alpha-phosphatidylethanolamine monomolecular layer at the surface of the silicon substrate through covalent bonds in a micro- runner system and then adsorbing the p-nitrophenyl ester-polyethylene glycol-(1,2-dioleoyl-3-glycerophosphatide ethanolamine) layer. Said protein chip is capable of applied in an imaging ellipsometry biosensor to explore target molecules capable of associating specifically therewith by covalently fixing ligands molecules at terminal tetrafunctional radicals of the protein chip surface PEG, wherein the surface morphology changes of the protein chip are capable of being observed in said imaging ellipsometry system, after both are associated. The bilayer lipids membrane surface modified protein chip in accordance with the present invention is capable of avoiding effectively a nonspecific adsorption, with its ligands molecules having excellent stability and being kept high biological activities.

The invention discloses application of hyperbranched polyglycerol modified magnetic nanoparticle microspheres in chemiluminescence immune assay. By utilizing the hyperbranched polyglycerol modified magnetic nanoparticle microspheres, a nanometer microsphere material of which the surface is hydrophilic and which is enriched in functional groups is prepared. When a chemiluminescence diagnostic agent based on the hyperbranched polyglycerol modified magnetic nanoparticle microspheres is actually used, the reagent can well inhibit nonspecific adsorption of proteins, and the antibody coupling amount is 2-3 times higher than that of commercial magnetic microspheres. In the project-based performance analysis, the diagnostic agent based on the magnetic microspheres can greatly enhance the reaction between antigens and antibodies. When the reagent is compared with a commercial magnetic microsphere reagent, the sensitivity is improved by 4-5 times, and the upper detection limit is improved by 1.3 times, so that the detection range is improved by nearly 6.5 times.

The invention discloses a method for preparing a dendritic-polymer-based microarray antibody chip and a product of the method. The method for preparing the dendritic-polymer-based microarray antibody chip comprises the steps that a glass slide is cleaned in ethyl alcohol, and then is immersed in an ethanol solution containing 3-glycidoxypropyltrimethoxysilane, and after annealing, the glass slide is immersed into a dry dichloromethane or tetrahydrofuran solution containing triethylamine and alpha-bromoisobutyryl bromide, and then is flushed with dichloromethane or tetrahydrofuran; nitrogen is blown into a methanol solution containing oligo (ethylene glycol) methacrylate and glycidyl methacrylate, 2,2'-bipyridyl and cuprous bromide are added into the methanol solution under ultrasonic waves, the glass slide is immersed into the methanol solution, and the methanol solution is sealed and stored in an inert and light-avoiding atmosphere box to obtain an activated carrier material; finally, a microarray chip sample application instrument is used for printing detection antibodies on the activated carrier material, standing is carried out, and the microarray antibody chip is obtained through washing with a phosphate buffer solution. The obtained microarray antibody chip is high in sensitivity, good in specificity and capable of being used for early stage screening of diseases.

The invention discloses a biological detector based on the interference effect of an ordered porous nanostructure film. The biological detector includes a detection pool, a sensing unit arranged in the detection pool, and an optical fiber spectrometer for detecting the reflection interference spectrum of the sensing unit. The sensing unit is a porous film with three-dimensional ordered nanopores, and the diameter of the nanopores is 20nm-500nm. The invention also discloses a method for detecting biomolecules by using the biodetector. When the substance to be measured is adsorbed on the pore surface of the ordered porous film as the sensing unit, it will change the spectral distribution of the reflected interference light, resulting in a spectral line shift, which can be used to quantitatively or qualitatively analyze the sample The concentration of substances in the medium, the attachment rate, the interaction, the change of the geometric size, etc.

The invention relates to a method for modifying the surface of a QCM wafer. The gold-plated QCM wafer is soaked with an ethanol solution of ethyl 3-mercaptopropionate, the surface is cleaned with ethanol and air-dried naturally; The treated QCM wafer is infiltrated with the substance solution, and the wafer surface is covered with a strip or grid mask. Under the induction of ultraviolet light, the polymer is bonded to the wafer surface to form an array structure; it is washed successively with absolute ethanol and deionized water. On the surface of the wafer, remove the unreacted bonded polymer; use the buffer solution containing the antibody to soak the wafer, use deionized water to clean the wafer, and store the wafer in the buffer solution for future use. The advantage of the present invention is that: a polymer film with a certain pattern structure is prepared on the surface of the QCM wafer by light-induced grafting technology, and antibody molecules are further grafted on the surface of the film to form a "antibody-antigen reaction" that can capture specific biomolecules. Antenna" array with high sensitivity.

The invention discloses 6-amino caproic acid cellulose ester as well as a synthetic method and application thereof. According to the 6-amino caproic acid cellulose ester, some hydroxyls of cellulose are substituted by a group as shown in the specification, and the total average degree of substitution of the group on each glucose unit ring is 0.6-1.2. The 6-amino caproic acid cellulose ester is synthesized from raw materials derived from a wealth of sources, the reaction conditions are gentle, the cost is low, the synthesized 6-amino caproic acid cellulose ester can be used as a surface modifying reagent for a polydimethylsiloxane micro-fluidic chip and physically adsorbed on the surface of a passage of the polydimethylsiloxane micro-fluidic chip to form a monomolecular film with positive charges, so that the surface hydrophilicity of the passage of the polydimethylsiloxane micro-fluidic chip can be improved, and nonspecific adsorption of protein on the surface of the passage of the micro-fluidic chip can be effectively inhibited, so high-efficiency reappearance and separation of analyte are realized.

The invention discloses a method to modify proteid chip surface, its character is fixing a layer of low molecular weight proteid molecule on the chip by normal method at first, then fixing the proteid chip which is needed to be fixed. The low molecular weight is 1X10 to the power 3 -9X10 to the power 5 Dalton. The method can modify the surface character of the proteid chip surface: on one hand, because of the covering of the low molecular weight molecule on the chip surface, the biology activity of the unfixed proteid molecule can be ensured, and at the same time, the aspecfic absorb is controlled on the surface; on the other hand, the low molecular weight molecule has amino, carboxyl, hydroxyl and other active group polar aminophenol on the proteid molecule surface, they can be used to fix porteid molecule directly after being activeted, and to form stable covalent bond.

The invention discloses a synthesizing method of phosphocholine chitosan derivant, which comprises the following steps: reacting chitose powder and disubstituted Choline phosphonate with different proportions in the composite system of alkaline, carbon tetrachloride and secondary alcohol at 0-40 Deg C for 12-24h; hydrolyzing through alkaline liquid to obtain the product with different coupling rate. The invention modifies biological function and compatibility, which can be applied in the drug and gene carrier, tissue engineering rack, biological medical film, aquagel and biological intelligent instrument domain.