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Method for preparing dendritic-polymer-based microarray antibody chip and product thereof

A technology of dendritic polymer and antibody chip, which is applied in the field of preparation of microarray antibody chip, can solve the problems such as detection limit not meeting practical application requirements, low sensitivity and poor specificity of microarray antibody chip, etc., and achieves high uniformity. , high selectivity detection, high sensitivity effect

Active Publication Date: 2014-09-10
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, one of the main problems of the microarray antibody chip is that its sensitivity is not high, and the detection limit cannot meet the requirements of practical applications; in many cases, the specificity is poor, and it is easy to cause false positive or false negative results
Therefore, the sensitivity and specificity of microarray antibody chips need to be improved urgently.

Method used

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  • Method for preparing dendritic-polymer-based microarray antibody chip and product thereof
  • Method for preparing dendritic-polymer-based microarray antibody chip and product thereof
  • Method for preparing dendritic-polymer-based microarray antibody chip and product thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0024] The preparation method of the microarray antibody chip based on dendritic polymer comprises the steps:

[0025] (1) Carrier material activation method: submerge the glass slide in ethanol and ultrasonically clean it for 30 minutes, take it out and immerse it in an ethanol solution with a mass fraction of 3-epoxypropyloxypropyltrimethoxysilane for 2 hours, Then vacuum annealing at 110°C for 2 hours, and then immersing the glass slide in dry dichloromethane with a mass fraction of 0.35% and 0.6% of triethylamine and α-bromoisobutyryl bromide for 2 hours, Finally, take out and rinse with dichloromethane for 3 times to obtain the cleaned slide; (The volume ratio of methanol:water in the methanol solution is 1:1), and nitrogen gas is bubbled into the solution for 20 minutes, and then 2,2'-bipyridyl and cuprous bromide are added under ultrasonic conditions to a final concentration of 3mg respectively / mL and 1.4mg / mL, then immerse the cleaned glass slide, seal the solution a...

Embodiment 2

[0029] The preparation method of the microarray antibody chip based on dendritic polymer comprises the steps:

[0030] (1) Carrier material activation method: immerse the glass slide in ethanol and ultrasonically clean it for 50 minutes, take it out and immerse it in an ethanol solution with a mass fraction of 3-epoxypropyloxypropyltrimethoxysilane for 1 hour, Then vacuum annealing at a temperature of 110°C for 1 hour, and then immersing the glass slide in dry dichloromethane with a mass fraction of 0.1% and 0.1% of triethylamine and α-bromoisobutyryl bromide for 2 hours, Finally, take out and rinse with dichloromethane for 3 times to obtain the cleaned slide; (the volume ratio of methanol:water in the methanol solution is 1:4), and nitrogen gas is bubbled into the solution for 15 minutes, and then 2,2'-bipyridyl and cuprous bromide are added under ultrasonic conditions to a final concentration of 1mg respectively / mL and 0.45mg / mL, then immerse the cleaned glass slide, seal ...

Embodiment 3

[0034] The preparation method of the microarray antibody chip based on dendritic polymer comprises the steps:

[0035](1) Carrier material activation method: immerse the glass slide in ethanol and ultrasonically clean it for 20 minutes, take it out and immerse it in an ethanol solution with a mass fraction of 3-epoxypropyloxypropyltrimethoxysilane for 4 hours, Then vacuum anneal at a temperature of 110°C for 4 hours, then immerse the glass slide in tetrahydrofuran solutions with a mass fraction of 1.0% and 1.2% of triethylamine and α-bromoisobutyryl bromide for 2 hours, and finally take out Rinse 3 times with tetrahydrofuran to obtain the cleaned glass slide; then prepare oligo(ethylene glycol) methacrylate and glycidyl methacrylate mass fractions respectively in 90% and 1% methanol solution (where methanol solution Methanol:water volume ratio is 4:1), and nitrogen gas is bubbled into the solution for 30 minutes, and then 2,2'-bipyridine and cuprous bromide are added under ult...

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Abstract

The invention discloses a method for preparing a dendritic-polymer-based microarray antibody chip and a product of the method. The method for preparing the dendritic-polymer-based microarray antibody chip comprises the steps that a glass slide is cleaned in ethyl alcohol, and then is immersed in an ethanol solution containing 3-glycidoxypropyltrimethoxysilane, and after annealing, the glass slide is immersed into a dry dichloromethane or tetrahydrofuran solution containing triethylamine and alpha-bromoisobutyryl bromide, and then is flushed with dichloromethane or tetrahydrofuran; nitrogen is blown into a methanol solution containing oligo (ethylene glycol) methacrylate and glycidyl methacrylate, 2,2'-bipyridyl and cuprous bromide are added into the methanol solution under ultrasonic waves, the glass slide is immersed into the methanol solution, and the methanol solution is sealed and stored in an inert and light-avoiding atmosphere box to obtain an activated carrier material; finally, a microarray chip sample application instrument is used for printing detection antibodies on the activated carrier material, standing is carried out, and the microarray antibody chip is obtained through washing with a phosphate buffer solution. The obtained microarray antibody chip is high in sensitivity, good in specificity and capable of being used for early stage screening of diseases.

Description

technical field [0001] The invention belongs to a preparation method of an immunochemical detection device, in particular to a preparation method of a microarray antibody chip based on a dendrite polymer, and a product prepared by the method. Background technique [0002] The microarray antibody chip immobilizes different antibody probe molecules on the carrier in an orderly, high-density, and addressable manner, through the specific capture of the target biomolecules by the antibody, and combined with a specific signal reading method such as fluorescence , electrochemistry, etc., an advanced technology for high-throughput detection of biomolecules, which is of great significance in the early screening of diseases, diagnosis and treatment effect evaluation, drug development, and basic life science research. In recent years, it has been widely used in academia and industry research interests. At present, one of the main problems of the microarray antibody chip is that its se...

Claims

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Application Information

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IPC IPC(8): G01N33/543
CPCG01N33/543
Inventor 李长明胡卫华刘英帅
Owner SOUTHWEST UNIVERSITY
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