215 results about "Microarray cgh" patented technology

The present invention provides a microarray chip for use in the analysis of various sample types. The microarray chips disclosed herein generally comprise a substrate covered with a coating material comprising a photoresist material, wherein the coating material is patterned to comprise a plurality of microstructures such as microwells and/or microcolumns. Methods for preparing and utilizing the microarray chips of the invention are further provided. The microarray chips of the instant invention find particular use in high-throughput assays.

The present invention relates to pathogen detection and identification by use of DNA resequencing microarrays. The present invention also provides resequencing microarray chips for differential diagnosis and serotyping of pathogens present in a biological sample. The present invention further provides methods of detecting the presence and identity of pathogens present in a biological sample.

The invention discloses a microarray chip without solid walls based on LSPR (Localized Surface Plasmon Resonance) and application thereof. The substrate of the microarray chip is in a hydrophilic and hydrophobic mode and comprises a base, a plurality of hydrophilic regions and hydrophobic regions, wherein the hydrophilic regions are arranged on the base; the hydrophobic regions separate the hydrophilic regions; the surfaces of the hydrophilic regions are provided with metal nanometer material layers with local area surface plasma resonance attributes; the outer regions of the hydrophilic regions are not provided with the solid walls; the surfaces of the hydrophobic regions are provided with hydrophobic material layers. The microarray chip disclosed by the invention can be obtained by combining a peculiar affinitive molecule for detecting a substance to be detected on the substrate provided by the invention. The microarray chip disclosed by the invention has the advantages of easiness and convenience for use, low detection cost, accurate result, reusability, and the like, can be used for detecting multiple substances, such as micromolecules, heavy metal ions, proteins, bacteria, viruses, and the like, and has wide application prospect in the fields of medical health, environmental monitoring, scientific experiments, and the like.

The invention relates to an immune gene prognosis model for predicting hepatocellular carcinoma tumor immune infiltration and postoperative survival time, and belongs to the technical field of biological medicines. The model can be used for evaluating the infiltration degree of immune cells in a tumor in clinical practice by detecting the expression levels of 22 specific immune related genes of ahepatocellular carcinoma patient, so that the model can be used for predicting hepatocellular carcinoma tumor immune infiltration in clinical practice and improve the prediction capability of the liver cancer immunotherapy response. The model can be used for judging the postoperative overall survival risk of a patient and guiding the formulation of a postoperative treatment strategy, and the corresponding microarray chip kit can realize the standardization and convenience of detection. Meanwhile, the immune gene prognosis model provided by the invention can increase the prediction accuracy andthe clinical net income of a hepatocellular carcinoma TNM staging system on the total survival time of three years and five years after operation. As a molecular marker for objectively and accuratelyevaluating the tumor immune state and poor prognosis risk of hepatocellular carcinoma, the model can realize accurate implementation of hepatocellular carcinoma immunotherapy and accurate prognosis prediction.

The present invention provides a protein micro-array surface plasma resonance imaging detection system and its detection method. Said system includes surface plasma resonance micro-array protein sensor, incident arm, reflecting arm and signal processing unit, the described sensor includes prism, micro-array chip and sample cell, between prism and micro-array chip a refractivity oil layer is coated, the incident arm is positioned at one side of the sensor, successively includes semiconductor laser, collimator, polarizer, attenuator and reactangular light diaphragm, the reflecting arm is positioned at another side of the sensor, and successively includes lens and CCD receiver, and signal processing unit includes signal processing circuit and computer.

A system, method and device for the detection of reactions between analytes, (e.g., DNA, biomolecules, or cells) and a second compound are disclosed. The present invention includes a coating of a fluorescent material having a fluorescence that changes with temperature. The fluorescent material is associated with a substrate, and can be used for any type of surface reaction that requires determination of temperature conditions at an interface between the surface and the reaction analyte subject to assay. The substrate may be, for example, a microarray chip or microplate, preferably suitable for use in high-throughput screening of biomolecules or cells. Substrates containing the fluorescent material also can be used to compensate for temperature variations in refractive index in optical sensors.

The invention belongs to the technical field of chemical analysis and discloses a method for preparing a microarray chip between a hydrophilic phase and a hydrophobic phase and carrying out mass spectrum imaging quantitative analysis by using the microarray chip. The method comprises the following steps: selecting a hydrophobic polymer; printing the hydrophobic polymer on conductive glass by adopting a screen printing technique according to a designed template to form a hydrophilic region and a hydrophobic region which are clear and uniform; uniformly paving a water solution of a mixture system to be detected in the hydrophilic region to form a chip; drying and curing the chip; uniformly paving a base material solution for ionizing the water solution of the mixture system to be detected on the surface of the chip; after the chip is dried, forming a sample; measuring a mass spectrum image of the sample by using a mass spectrum imaging method; processing the image obtained by mass spectrum imaging of the sample in the same area through using a mathematical method, and carrying out the quantitative analysis. The method is high in automation degree, simple in operation and high in sensitivity; the detection sensitivity of a material to be detected reaches 10-15mol/L; the use amount of the sample is less and is as low as a microliter-grade.

The present invention provides aptamer libraries with pre-defined secondary structures that can be used for oversampled screening for affinity binding. In one embodiment, a multiplex approach is employed to divide the library into degenerate subsets that are immobilized on multiple locations of a solid support such as a microarray chip

The nucleic acid sequencing process based on nucleic acid micro array chip is for sequencing genome and detecting gene transcription and expression spectrum of some specific species, and detecting various genome DNA modifying spectrum. Different DNA segments to be sequenced are fixed onto one solid chip to form nucleic acid micro array, and each of the DNA segments is made to contain the same common sequence as the complementary sequence of the sequencing primer. After crossing the common sequencing primer and the chip, some mixed nucleotide solution with certain ratio of fluorescent marker ddNTP and dNTP is injected to the chip to terminate on-chip base reaction with a micro flow controller by means of Sanger end terminating process. Once the termination is finished, one fluorescent signal is obtained through scanning to obtain the base extending information in different sample points on the chip. All the sequencing maps are finally superposed to obtain the complete nucleic acid segment information.

A fluidic nano/micro array chipset comprises a microarray filling chip and a nano/micro array stamping chip. There are a plurality of sample containers and a plurality of nano/micro channels on the top of the microarray filling chip, and a plurality of nano/micro-scaled micro filling holes on the bottom of the microarray filling chip. Each nano/micro channel is connected to one of the sample containers and leads the sample solution in that sample container to the corresponding micro filling hole. The nano/micro array stamp chip comprises a plurality of stamping heads arranged in an array pattern, with a body part of the stamp chip and a plurality of space channels forming hydrophobic areas. Each sample solution is stored in the body of the stamp chip, and is transported by the corresponding stamping head to the stamping part of this stamping head.

A computer based method for analyzing microarray chip information. A computer implemented artificial neural network (ANN) is trained by back propagation of error using a set of training microarray chip input vectors to create a trained ANN. At least one set of test data is applied to the trained ANN to generate a prediction. The trained ANN numerically analyzing with respect to a subset of the input vectors to identify those elements of the input vector which are most effective in obtaining the prediction. the set of input vectors is reduced in dimension to contain data only from those genes found most effective in obtaining the prediction to form a dimensionally reduced set of input vectors. The neural network is retrained using the dimensionally reduced set of input vectors by back propagation of error to generate a retrained network. The at least one set of test data is reapplied to the retrained neural network to generate a second prediction.

The invention discloses a semiconductor chip used for characteristic spectrum discrimination and a manufacture method thereof, as well as a characteristic spectrum discrimination device and a biochip scanning device using the chip to measure absorption spectroscopy and fluorescence spectrum. According to the invention, the chip used for characteristic spectrum discrimination comprises an array semiconductor optical sensor, the array structure of which comprises at least a detection pixel and at least a reference pixel; the chip also comprises at least one optical filter plate, and the surface of each detection pixel is covered by a corresponding optical filter plate. Each optical filter plate has characteristic wavelength, thus leading the wavelength which takes the characteristic wavelength as the center in a predetermined width range to transmit through the optical filter plate, while the wavelength beyond the width range not to transmit. According to the invention, the semiconductor technology can be used for manufacturing the chip for characteristic spectrum discrimination at low cost and an absorption spectroscopy apparatus, a fluorescence spectrum apparatus and a biological microarray chip, with small volume, can be conveniently manufactured at low cost.

This invention relates to a plastic substrate for a microarray chip, which is characterized in that an aminoalkylsilane with an aldehyde group derived from glutaraldehyde and introduced onto an amino group of the aminoalkylsilane exists on a surface of the plastic substrate, and also to a process for its production and a method of its use. Substrates according to the present invention have no variations in the immobilized amount of DNA fragments upon immobilization of DNA fragments, permit reproducible hybridization with high efficiency, and are excellent in storability.

A process for high-flux screening, capturing and separation of target moleculae from complex substance (Chinese medicine or chemical mixer) includes preparing a ds DNA microarray chip to make its probe have a binding sites of dsDNA bindin, measuring the affinity of the sites, creating the standard affinity parameter system, mixing the microarray chip with said complex substance, reacting, detecting the affinity variation, screening the varied targets, linking them to the surface of chromatographic column, filling the complex substance in it, capturing the effective moleculae by probes, eluting and collecting the effective moleculae.

The invention provides methods and compositions for use in the diagnosis and management of cancer, particularly breast cancer. The invention utilizes differential gene expression profiles in tumor associated stroma and normal stroma to compile a stroma derived prognostic predictor that classifies breast cancer patients according to clinical outcome. The application provides nucleic acids, antibodies, microarray chips and kits for use with the methods described in the application.