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Method of high flux screening, capturing and separating target molecule from complex composition matter such as traditional Chinese medicine and chemical mixture

A high-throughput, material technology, applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve problems such as failure to achieve expected results, inability to reveal drug effect gene expression changes, etc.

Inactive Publication Date: 2005-03-09
王进科 +2
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Problems solved by technology

However, the limitation of this kind of research is that it cannot reveal how the drug causes the change in the expression of the effector gene. Therefore, the specific target, pathway, and method of drug action, as well as the specific components of the drug’s action are unknown. The identification and separation of components are still powerless
So far, the progress of this research has not achieved the results that people expect in drug research

Method used

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  • Method of high flux screening, capturing and separating target molecule from complex composition matter such as traditional Chinese medicine and chemical mixture
  • Method of high flux screening, capturing and separating target molecule from complex composition matter such as traditional Chinese medicine and chemical mixture
  • Method of high flux screening, capturing and separating target molecule from complex composition matter such as traditional Chinese medicine and chemical mixture

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Embodiment Construction

[0062] 1. Preparation of double-stranded nucleic acid microarray chip

[0063] The implementation of the technology of the present invention will be described below by taking the example of the reaction between the protein NF-κB and the double-stranded DNA microarray chip prepared on the glass slide.

[0064] Design and chemical synthesis of single-stranded oligonucleotides: use Chinese patent (02112780.8) technology to design and synthesize probe single-stranded oligonucleotides with solid-phase chemical synthesis technology, and prepare double-stranded DNA microarray chips for detecting NF-κB. The synthetic probe single-stranded oligonucleotide building block is as follows: 5'NH 2 -TTTTAGTTGAG CGTA CGC - GCGTACG -OH 3'; the DNA sequence in the box is an example NF-κB binding site, and the other site sequences are shown in Table 1. The underlined sequences are complementary sequences in both directions. Use synthetic oligonucleotides to prepare NF-κB detection double-...

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Abstract

A process for high-flux screening, capturing and separation of target moleculae from complex substance (Chinese medicine or chemical mixer) includes preparing a ds DNA microarray chip to make its probe have a binding sites of dsDNA bindin, measuring the affinity of the sites, creating the standard affinity parameter system, mixing the microarray chip with said complex substance, reacting, detecting the affinity variation, screening the varied targets, linking them to the surface of chromatographic column, filling the complex substance in it, capturing the effective moleculae by probes, eluting and collecting the effective moleculae.

Description

1. Technical field: [0001] In the field of basic molecular biology and biomedicine, the present invention proposes a new method for high-throughput screening, capture and separation of target molecules from complex components such as traditional Chinese medicine and combinatorial chemical mixtures, that is, using the detection of specific DNA / The double-stranded nucleic acid or peptide nucleic acid microarray chip of the RNA binding protein reacts with the DNA / RNA binding protein to establish the binding affinity of the DNA / RNA binding protein to the double-stranded nucleic acid or peptide nucleic acid target on the chip as a standard reference system; React the complex component substances with the microarray chip. After the reaction, the microarray chip continues to react with the DNA / RNA binding protein. After the reaction of the complex component substances with the microarray chip, those that bind to DNA / RNA are screened out by comparing the standard reference system. Mi...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 王进科陆祖宏李同祥
Owner 王进科
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