77results about How to "High immobilization efficiency" patented technology

The invention discloses a compound immobilization method for fructosyl transferase, and belongs to the field of biotechnologies. The technology has the advantages that fructosyl transferase extraction technological equipment is simple; the fructosyl transferase is embedded by calcium alginate after chitosan covalence immobilization; the problems of forming and integer of chitosan covalence immobilized enzyme can be solved; the immobilization efficiency of the enzyme is high (enzyme activity recovery rate reaches above 70%); the enzyme can be used repeatedly for more than 85 times during batch reaction.

The invention belongs to the technical field of novel materials and particularly relates to a biocompatible metal-organic framework material glutamic acid-zinc and preparation and an application thereof. The biocompatible metal-organic framework material glutamic acid-zinc is obtained through dropwise adding a disodium glutamate water solution into a methanol solution of Zn(NO3)2. The method has the characteristics of being high in glutamic acid-zinc crystal growth speed and mild in preparation condition, and the prepared product is good in crystal form, can be applied to a series of enzymes containing abundant amino, such as lipase, protease and epoxide hydrolase, and has the potential as a protein drug carrier. The product is added to an aspergillus niger lipase phosphate buffer solution modified by EDC or EDC.HC and NHS for shock immobilization to obtain the immobilized aspergillus niger lipase. The method is high in immobilization efficiency, high in enzyme recovery rate, simple in operation and mild in condition.

The invention discloses a method for preparing magnetic covalent immobilized enzyme carriers, which relates to the field of biological chemistry and comprises the following steps that: firstly, oleic acid covered Fe3O4 with a magnetic structure is prepared, and then, Fe3O4 carries out polymerization reaction with olefine acid ester and subsequently and sequentially carries out reaction with hydrazine hydrate and nitrous acid to obtain activated immobilized enzyme carriers in nitrine magnetic nanometer structures. The method of the invention has the advantages that the process is simple, the operation cost is low, the enzyme carrying quantity is high, the stability is good, the reaction is easy to control and operate, the enzyme carrying capacity is as high as 250mg / g, and the original enzyme performance of the immobilized enzyme can almost be maintained at 100 percent.

The invention relates to a technique method of immobilized candida antarctica lipase B, which adopts an organic solvent as an immobilizing medium and realizes the immobilization of lipase in microaqueous phase. The technique method comprises the following steps of: (1) pretreatment of resin; (2) preparation of immobilized enzyme: the pretreated resin is added into a reactor and an organic medium is added; and after standing, then zymogen solution is added according to the mass ratio of the candida antarctica lipase B and the resin, which is equal to 1:60-1:100; the reactor is sealed and then arranged in a shaking bath with constant temperature of 25-35 DEG C for 1h-3h; after vacuum freezing and drying, the immobilized candida antarctica lipase B is prepared. The method for preparing the immobilized enzyme has low consumption of resolvase and high immobilization efficiency and avoids the considerable waste of resolvase caused by an aqueous-phase absorption method, thereby reducing immobilization cost; a carrier has low price and is easily obtained; furthermore, the method has simple technique, mild condition, low loss in enzyme activity and enzyme activity recovery rate of 83.3 percent.

The invention relates to the field of polymer materials. The gel raw materials of an immobilized cell carrier which is prepared from bentonite and biological polysaccharide complex gel and disclosed in the patent with the application number of 200710151906.3 comprise carrageenan and guar gum complex natural plant polysaccharide. Compared with the prior art, various properties of the immobilized cell carrier are enhanced, but the curing temperature is higher, and the thermal decomposition temperature as well as the mechanical strength are lower, thereby causing more loss of microbial activities and larger damage rate of a cell carrier ball. In order to further improve the properties, the method adopts the following steps: preparing a water solution with the consistency of 2.4-3.0 percent from tamarind seed gum and sodium alginate complex biological polysaccharide; curing the water solution after cooling in a calcium chloride solution with the consistency of 2.5-3.5 percent and a sodiumpyroborate solution with the consistency of 2.5-3.5 percent for the second time; and finally, keeping the cured solution in physiological saline. The prepared immobilized cell carrier has the advantages of high mechanical strength, thermal decomposition temperature and microbial curing rate as well as low damage rate and cost.

Technology for fixing fungi and bacterium with vermiculite as carrier is carried out by modifying while moistening for vermiculite as carrier material, steam sterilizing at high-temperature and high-pressure, inoculating mixed bacterium, and multiplication culturing to obtain the final product. It's simple, porous and efficient, has better growth environment, degradation and resistance to poisonous, larger specific area and competition ability.