Immobilized enzyme of core-shell structure as well as preparation method and application of immobilized enzyme

Abstract

The invention belongs to the field of immobilized enzymes and in particular relates to an immobilized enzyme of a core-shell structure as well as a preparation method and application of the immobilized enzyme. The preparation method comprises the following steps: preparing an enzyme solution from zymoprotein, adding a modifier into the enzyme solution, uniformly mixing, and reacting for 2-10 hoursto obtain a surface double bond modified zymoprotein; and adding a monomer in an amount of 1-10 times that of the mass of the solution into the double bond modified enzyme solution, performing vortexblending uniformly, removing dissolved oxygen, sequentially adding a cross-linking agent, an initiator and a catalyst, reacting the solution at room temperature for 1-5 hours, dialyzing and purifying, thereby obtaining the immobilized enzyme of the core-shell structure. Compared with the traditional immobilized enzyme method, the method disclosed by the invention has the advantages that the enzyme is immobilized on a small scale, and the enzyme immobilization efficiency is improved, so that all the enzyme molecules are embedded and fixed by the finally formed 'shell' structure. Meanwhile, theprepared immobilized enzyme system has excellent properties such as high enzyme activity retaining rate and environmental stability, wide application field and the like.

Description

technical field [0001] The invention belongs to the field of immobilized enzymes, in particular to a shell-core structure immobilized enzyme and its preparation method and application. Background technique [0002] Enzymes are a class of highly efficient and specific biocatalysts, which are widely used in many fields such as biopharmaceuticals, food processing, environmental protection, and clean energy development. Under the new situation of vigorously advocating the concept of sustainable development, developing green and energy-saving industrial production methods and using renewable resources and energy, enzyme catalysis will play an increasingly important role in the production and processing of bulk commodities and fine chemicals. . [0003] Enzymes are special organic substances (proteins, RNA) with catalytic activity and high selectivity produced by living cells, and the chemical nature of most enzymes is protein. Limited by the characteristics of the protein itsel...

AI Technical Summary

Problems solved by technology

Due to its own disadvantages, traditional methods, such as immobilized enzymes by physical adsorption, have limited immobilization capacity for enzymes and poor stability; covalent binding methods require that the group at the binding site must not be the active center of the enzyme, and the immobilized enzymes usually obtained The recovery rate of enzyme activity is low; the cross-linking method requires the combination of the enzyme and the carrier material, the reaction conditions are relatively severe, and the activity of the immobilized enzyme is low; the encapsulation method is difficult to control the network size of the carrier material, and the enzyme is easy to leak

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