Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of petroleum degrading bacteria solid inoculant and method for restoring petroleum-polluted soil by using prepared solid inoculant

A technology of petroleum-degrading bacteria and solid bacterial agent, which is applied in the field of microbial remediation of petroleum-contaminated soil, and can solve problems such as unguaranteed actual effects, unrealized industrialized production, suboptimal viability, and unsatisfactory degradation efficiency.

Active Publication Date: 2015-03-25
QINGDAO AGRI UNIV
View PDF2 Cites 23 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The large-scale promotion of microbial remediation of oil-contaminated soil technology requires a large number of microbial agents. Domestic research on oil-degrading agents has only carried out small-scale laboratory cultivation, and has not achieved industrial production.
[0007] There have been reports on methods for screening petroleum-degrading bacteria using petroleum hydrocarbons as the only carbon source, but most of them use a single petroleum hydrocarbon or several petroleum hydrocarbons and their intermediates as carbon sources for screening. There are many kinds of actual conditions, so the viability and degradation efficiency of the strains screened under this condition in the actual oil-contaminated soil are not ideal, and the actual effect of using them in the treatment project of oil-contaminated soil cannot be guaranteed.
[0008] Most of the existing screening techniques for petroleum-degrading bacteria are based on laboratory research. A single-component petroleum hydrocarbon is used as a substrate to screen bacteria, and then the petroleum hydrocarbon is used as a substrate to evaluate the degradation effect of the screened bacteria. It is only a small-scale laboratory test for bacterial culture

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of petroleum degrading bacteria solid inoculant and method for restoring petroleum-polluted soil by using prepared solid inoculant
  • Preparation method of petroleum degrading bacteria solid inoculant and method for restoring petroleum-polluted soil by using prepared solid inoculant
  • Preparation method of petroleum degrading bacteria solid inoculant and method for restoring petroleum-polluted soil by using prepared solid inoculant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Screening of Efficient Petroleum Degrading Bacteria Strains

[0069] 1. Collection of oil-degrading indigenous bacteria sources

[0070] The oil-contaminated soil near the oil storage tank of the Shengli Oilfield refinery in Shandong Province was used as the source of indigenous oil-degrading bacteria. Sampling method: Use a small shovel to take soil samples with a thickness of 10cm, mix them evenly after sampling at multiple points, take 3kg soil samples and bring them back to the laboratory for later use.

[0071] 2. Preparation of Petroleum Polysorbate-80 Enhanced Solution

[0072]After the surfactant reaches the critical micelle concentration (CMC) in the aqueous solution, the solubility of some water-insoluble or slightly soluble substances in the micellar solution can be significantly increased to form a transparent colloidal solution. This effect is called solubilization. (solubilization). Polysorbate-80 (polyoxyethylene sorbitan fatty acid ester) is a water-s...

Embodiment 2

[0120] Liquid fermentation production of petroleum degrading bacterial agent

[0121] 1. Primary seed cultivation

[0122] Under sterile conditions, the mixed strain 2 of petroleum-degrading bacteria purified in Example 1 was inoculated in the slant culture medium of the test tube. Culture medium composition Per 1000ml sterile water: petroleum polysorbate-80 enrichment solution containing petroleum 0.125g equivalent (wherein petroleum mass concentration is 2%, polysorbate-80 mass concentration is 4%, remainder is water.), beef Cream 3g, peptone 10g, NaCl 5g, agar powder 20g, pH value is 7, sterilized at 121°C for 20 minutes. The culture conditions are: 30°C, cultured for 24 hours, and used as first-class seeds.

[0123] 2. Secondary seed cultivation

[0124] Under sterile conditions, take the two-ring primary seeds and put them into a 500ml Erlenmeyer flask filled with 200ml medium. Shake flask medium composition per 1000ml sterile water: petroleum polysorbate-80 enrichmen...

Embodiment 3

[0132] Combination of oil-degrading solid compound bacterial agent and plants to remediate oil-contaminated soil

[0133] 1. Selection and processing of experimental bases

[0134] This test selects the land near the oil extraction machine of an oil production plant in Dongying City. The land is located near the oil extraction machine and is polluted by oil. The total hydrocarbon content of oil is 4g / Kg. The basic physical and chemical properties of the soil are shown in Table 5. In the present invention, alfalfa-microbes are used to jointly restore oil-contaminated soil, and the planting density is about 8000 plants / mu. The prepared oil-degrading bacteria solid bacterial agent is added to the oil-contaminated soil at a rate of 10-100 kg / mu, and plowed. 0-30cm topsoil and mix well. When alfalfa is planted, the soil is covered with a film to retain moisture, and a controlled-release fertilizer is applied to ensure the nutrients needed for normal plant growth. At the seedling ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a preparation method of a petroleum degrading bacteria solid inoculant. The preparation method comprises the following steps: A, screening and domestication of the petroleum degrading bacteria; B, preparation of a seed culture solution; C, fermentation of the solid inoculant; and D, drying, pulverization, measurement and packaging of composted products. The petroleum degrading bacteria solid inoculant has the beneficial effect of improving the petroleum dissolution effect by taking a nonionic surfactantpolysorbate-80 (tween 80) as a solubilizer. The preparation method has the advantages that solid fermentation raw materials are easy to get and the process is relatively simple; and the solid inoculant contains a large amount of carbon and nutrient elements so as to provide more proper matrix for the growth of the bacteria, has strong affinity for the microorganisms, has immobilization efficiency, is capable of improving the competitiveness and degrading efficiency of the added microorganisms and indigenous microorganisms, and is convenient to transport and operate agriculturally and suitable for the large-scale in-situ biological remediation of the petroleum-polluted soil.

Description

technical field [0001] The invention relates to the technical field of microorganism restoration of petroleum-contaminated soil, in particular to a method for preparing a solid bacterial agent of petroleum-degrading bacteria and a method for repairing oil-contaminated soil by the solid bacterial agent prepared therefrom. Background technique [0002] With the rapid development of economy, soil environmental problems caused by humans are becoming more and more serious. As a non-renewable resource for human beings, soil is the basis for human survival. The toxic and harmful substances discharged by industry and agriculture are increasing day by day, and the organic pollution caused by the soil is on the rise. Among them, the oil discharged in the process of oil development, transportation, processing, storage and transportation is particularly harmful to the soil environment. Petroleum is a mixture of various chemical substances with different chemical properties, mainly inc...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/36B09C1/10
CPCB09C1/10C12N1/36
Inventor 郑庆柱田侠
Owner QINGDAO AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com