244 results about "Cell carrier" patented technology

The present invention relates to a device comprising a cell carrier portion containing regenerative cells, e.g., stem and progenitor cells, and a cell carrier containment portion. The device is useful for the treatment of bone related disorders, including spinal fusion related disorders and long bone or flat bone related defects. The device may be used in conjunction with disclosed automated systems and methods for separating and concentrating regenerative cells.

Cell-loaded devices or prostheses having various applications such as insertion into body passages are disclosed. The prostheses include cell carrier portions which are compatible with living tissue and which are loaded with therapeutic cell populations, and the prostheses can be applied within or replace one or more of narrow segments, environments which may be difficult to access or luminal areas of the body such as parts of blood vessels. In the context of blood vessels, the cell-loaded devices or prostheses can line or otherwise treat with therapeutic cell populations inner walls of damaged blood vessels and surrounding parenchyma or other organs.

The invention discloses a preparation method of sericin hydrogel, the method is as follows: first, weighting domestic silkworm fibroin deletion form mutation variety silkworm cocoon for LiBr or LiCl extraction and dialysis and purification to obtain a non-degradable sericin water solution with a mass percentage concentration of 0.1-4%; concentrating the sericin water solution to 1.5-10%, adding a cross-linking agent to the concentrated sericin water solution (wherein 2-500muL of the cross-linking agent is added into each L of the sericin water solution), fully mixing, and placing at 4-45 DEG C for 5 seconds to 36 hours to obtain the sericin hydrogel. The sericin hydrogel has biological activity and multiple functions, can be used as carrying growth factors, drugs and cell carriers treatment, and can be applied in repair of a variety of soft tissue injuries, including but not limited to skin damages, muscle damages, injuries of blood vessel, nerve injuries, myocardial damages, and the like, and treatment of diseases.

An apparatus for collecting or culturing cells or cell colonies includes: a common substrate formed from a flexible resilient polymeric material and having a plurality of wells formed therein; and a plurality of rigid cell carriers releasably connected to said common substrate, with said carriers arranged in the form of an array, and with each of the carriers resiliently received in one of the wells. A method of collecting or culturing cells or cell colonies with such an apparatus is carried out by depositing a liquid media carrying cells on the apparatus so that said cells settle on or adhere to said the carriers; and then (c) releasing at least one selected carrier having said cells thereon by gradual application of release energy to each carrier from the cavity in which it is received (e.g., by pushing with a probe).

The present invention relates to a process of producing a collagen gel comprising collagen fibers cross-linked with each other by a cross-linking agent on the way of fibrosis of collagen, and a collagen gel comprising the cross-linked collagen fibers obtained by the process. The collagen gel comprising cross-linked collagen fibers of the present invention has high strength and thermal stability and can be widely used as cell carriers and medical materials.

To improve a cartilage replacement implant for the biological regeneration of a damaged cartilage area of articular cartilage in the human body, comprising a cell carrier which has a defect-contacting surface for placement on the damaged cartilage area and is formed and designed for colonization with human cells, so that after implantation of the cartilage replacement implant, formation of a gap between adjacent contact surfaces of the implant and surrounding recipient tissue is minimized, it is proposed that the cell carrier rest with surface-to-surface contact on a carrier and be joined to the carrier at a cell carrier surface that faces away from the defect-contacting surface. A method for producing a cartilage replacement implant is also proposed.

A wireless terminal receives signaling information, pertaining to a reference signal transmission in at least one specifically designated sub frame, the signaling information including a list, the list including base station identities. The terminal determines, from at least one of the base station identities in the list, the time-frequency resources associated with a reference signal transmission intended for observed time difference of arrival (OTDOA) measurements from a transmitting base station associated with said one base station identity. The time of arrival of a transmission from the transmitting base station, relative to reference timing, is measured. The wireless terminal can receive a command from a serving cell to start performing inter-frequency OTDOA measurement on a frequency layer containing reference signals, the frequency layer distinct from the serving frequency layer, the serving frequency layer not containing positioning reference signals. The wireless terminal can perform OTDOA measurements subsequent to the reception of the command on a carrier frequency different from the serving cell carrier frequency. A base station transmitter can jointly schedule a reference signal transmission from a plurality of base station transmitters for the purpose of OTD estimation enhancement, and transmit identical reference signals from the plurality of base station transmitters, the reference signals being identical both in the signal sequence and time-frequency resources used for transmission.

The invention relates to a virus preparation or vaccine production method by culturing cells with a polyester fiber carrier. The method particularly comprises the following steps: 1) preparing a polyester fiber cell culturing carrier by adopting a processing method; 2) in a cell culturing system, culturing proliferation cells in a cell proliferation culture solution with the treated polyester fiber cell culturing carrier; 3) changing a cell maintaining culture solution after the cells grow to a certain density or number, and inoculating virus to infect cells; 4) amplifying virus; 5) harvesting virus fluid; 6) inactivating or implementing living vaccine treatment according to the required preparation type; 7) concentrating, purifying and cracking the virus fluid; 8) adding a stabilizing agent and an immunoadjuvant; and 9) packaging vaccine. According to the method, a material which has low price and is suitable for wall attaching growth of the cells is used to make the cell culturing carrier, carry out cell culture and prepare virus or produce vaccine, so that the cost of the cell carrier is greatly reduced.

The invention provides supermolecular hydrogel containing chitosan and gelatin as well as a preparation method and an application of the supermolecular hydrogel and belongs to the field of biomaterials. The supermolecular hydrogel contains a part formed by combining chitosan modified with a beta-cyclodextrin carboxylated derivative and gelatin under the host-guest interaction; chitosan is graftedwith the beta-cyclodextrin carboxylated derivative through an amide group. The preparation method comprises the steps as follows: the chitosan is grafted with the activated beta-cyclodextrin carboxylated derivative through the amide group, then, the gelatin is added, so that the grafted beta-cyclodextrin carboxylated derivative and an aromatic group of the gelatin are in host-guest interaction, and the supermolecular hydrogel containing the chitosan and the gelatin is obtained. The supermolecular hydrogel containing the chitosan and the gelatin has shear thinning and self-healing characteristics and can be used as an injectable cell or drug carrier. The beta-cyclodextrin group in hydrogel and the aromatic group in the gelatin can be used in cell carriers and tissue engineering materials through the host-guest interaction.