54 results about "Candida antarctica" patented technology

The invention discloses a method for enriching n-3 polyunsaturated fatty acids by an enzymatic method, and belongs to the field of oil and fat deep processing. The method of the invention comprises: taking oil, water (alcohol) solution and lipase to react in a reactor, wherein the lipase includes one or more lipases derived from Candida cylindracea and lipase Candida antarctica lipase A, And the order of adding lipase is not fixed. At present, the content of saturated and monounsaturated fatty acids in the glycerides obtained by enzymatic catalytic hydrolysis or alcoholysis enrichment PUFA reaction is still relatively high; The monounsaturated fatty acid has specificity, and further reduces the non-PUFA fatty acid content in the glyceride product, thereby obtaining a glyceride product with a higher content of PUFA.

Shown and described is a composition and a method to prepare a dopant-free photoluminescent hydrogel with synthetic polymers are disclosed. The hydrogel can be synthesized in one embodiment by incorporating an amino acid to a citric acid based polyester oligomer followed by multiple crosslinking group functionalization through a transesterification reaction using an enzyme such as Candida antarctica Lipase B (CALB) as a catalyst. The hydrogels are injectable, degradable, and their mechanical and photoluminescent properties are tunable. An in vivo study shows that the hydrogel emits strong fluorescence under visible light excitation and can completely degrade over time.

A process for the preparation of an acid of formula (I), as the individual (R) enantiomer or (S), or a salt thereofwherein R is a protected amino group; and the asterisk * denotes the stereogenic carbon atom, comprising contacting an ester of formula (II), as mixture of (R,S) enantiomers, or a salt thereof,wherein R1 is straight or branched C1-C6 alkyl, optionally substituted with phenyl; (the asterisk * and R defined above), with a lipase from Candida antarctica, under conditions effective to obtain a mixture comprising an acid of formula (I), as the individual (R) enantiomer, and an ester of formula (II), as the individual (S) enantiomer; the subsequent hydrolysis of the latter to obtain an acid of formula (I), as the individual (S) enantiomer; and, if desired, the conversion of an acid of formula (I), either as the (R) or (S) enantiomer, to a salt thereof.

A process for the synthesis of Sofosbuviris provided comprising the steps of selectively mono-deacetylating a compound of formula (V)enzymatically using a resin supported lipase B derived from Candida Antarctica to obtain a compound formula (IV),then converting the compound of formula (IV) to a compound of formula (II)by reacting the compound of formula (IV) with a compound of formula (III), andthen converting the compound of formula (II) to Sofosbuvir of formula (I) by deacetylation reaction.

The invention belongs to the technical field of biochemical engineering and in particular discloses an immobilized enzyme for oriented catalysis of an esterification reaction and a preparation methodand application of the immobilized enzyme. The method comprises the following steps: mixing candida antarctica lipase with an HEPES (hydroxyethyl piperazin ethanesulfonic) buffer so as to obtain an HEPES-lipase liquid; and mixing the obtained HEPES-lipase liquid with GMP (guanine nucleotide) or AMP (adenine nucleotide) mother liquor so as to obtain a mixed solution, further uniformly mixing the mixed solution with metal ions, performing filtering, and performing freeze drying, so as to obtain the immobilized enzyme. The immobilized enzyme preparation method disclosed by the invention is simple, and the obtained immobilized enzyme is high in enzyme activity, is capable of effectively catalyzing the esterification reaction, in addition, is good in repeated utilization, but cannot catalyze alcoholysis reactions, has an oriented catalysis property, that is, is capable of catalyzing the esterification reaction but not alcoholysis reactions, and thus has very good application prospects.

The invention relates to a method for preparing (S)-1-(1-naphthyl)ethylamine by an enzyme method. According to the method, Candida antarctica lipase B is adsorbed by macroporous resin, and cross-linking is carried out by using glutaraldehyde so as to obtain immobilized Candida antarctica lipase B. The immobilized lipase serves as a catalyst, toluene serves as a solvent, ethyl acetate serves as an acyl donor, (S)-1-(1-naphthyl)ethylketoxime is selectively esterified, (R)-1-(1-naphthyl)ethylamine is not esterified, and a racemation reaction is carried out in the presence of a racemation catalyst and hydrogen gas. According to the method, the immobilized Candida antarctica lipase B can be used for completely converting 1-(1-naphthyl)ethylketoxime into (S)-1-(1-naphthyl)ethylamine ethyl ester at a relatively high temperature, and the reaction has the characteristics of short reaction time, high product optical purity, and the like.