82 results about "Adenine nucleotide" patented technology

A composition comprising a novel Ca2+-activated, [ATP]i-sensitive nonspecific cation (NCCa-ATP) channel is described. The channel is found in mammalian neural cells and exhibits a different sensitivity to block by various adenine nucleotides, and is activated by submicromolar [Ca]i. The NCCa-ATP channel is activated under conditions of ATP depletion, which causes severe cell depolarization, followed by cell swelling. The NCCa-ATP channel is regulated by a sulfonylurea receptor and is inhibited by sulfonylurea compounds glibenclamide and tolbutamide. Methods employing compositions comprising the NCCa-ATP channel to screen for compounds that block the channel and the use of such antagonists as therapeutics in preventing brain swelling and damage are described. In addition, methods employing compositions comprising the Kir2.3 channel to screen for compounds that open the channel and the use of such antagonists as therapeutics in preventing brain swelling and damage are described.

The invention relates to the technical field of detection sensors and particularly relates to an electrochemical aptamer electrode for kanamycin detection. Graphene-polyaniline compound, polyamide-amine dendritic high molecule-gold nano compound, kanamycin anti-body are modified on a glass carbon electrode in sequence from bottom to up; the surface of the electrode is sealed with BSA (Bovine Serum Albumin) and single chain DNA (Deoxyribose Nucleic Acid) containing 10 adenine nucleotide. The preparation method is simple, stable in performance and good in electrode repeating performance and is suitable for practical applications of kanamycin detection in food safety and bio-sensor industrialization; the prepared electrode is low in technical cost and is suitable for the requirement of low cost in industrialization; according to a sandwich type electrochemical sensing system fixed by using the glass carbon electrode as a fixing carrier and based on a DNA aptamer, rapid and online kanamycin detection in food is realized and the detection limit is 4.6x10<-6>mu gmL<-1>.

The invention discloses a microRNA-328 and the application of antisense nucleotide thereof for diagnosing and controlling heart diseases; in the invention, a plurality of experiments prove that the microRNA-328 is changed and has pathopoiesia function in heavy heart diseases (atrial fibrillation, ischemia-reperfusion or myocardial fibrosis), and the antisense nucleotide AMO-328 of the microRNA-328has prevention and cure functions to the heart diseases, the microRNA-328 is used as a novel drug effect target spot, so as to finally determine and detect that the peripheral blood microRNA-328 canbe used as a novel biological marker which is used for diagnosing and preventing the heart diseases; in addition, the peripheral blood microRNA-328 expression level of a patient can be detected through fluorescent quantitative PCR and the nanometer microballoon technology enhanced by silver staining, so as to be used for diagnosing and preventing the heart diseases such as atrial fibrillation, long QT syndrome, ischemia-reperfusion or myocardial fibrosis and the like.

The invention relates to a nanometer gold complex, as well as a preparation method and an application of the nanometer gold complex. Specifically, the nanometer gold complex provided by the invention comprises nanometer gold particles and a functional molecule, wherein the functional molecule is bound to the surfaces of the nanometer gold particles through an oligonucleotide binding region which is rich in adenine nucleotide or the analogue of the adenine nucleotide, and the functional molecule comprises the oligonucleotide binding region which is rich in the adenine nucleotide or the analogue of the adenine nucleotide, a functional region and a transition area located between the binding region and the functional region. The invention also provides the preparation method and the application of the nanometer gold complex.

The present invention relates to adenine nucleotide translocator 2 (ANT2) siRNA (small interfering RNA) or ANT2 shRNA (short hairpin RNA) suppressing the expression of ANT2 gene expression and anticancer agent containing the same. Furthermore, the present invention relates to methods for treating breast cancers or stem cells of a breast cancer by treating the same with ANT2 siRNA or ANT2 shRNA. In addition, the invention provides a method for inhibiting metastasis of breast cancer cells with ANT2 siRNA or ANT2 shRNA.

The invention discloses a catalytic light mark and a preparation method thereof, and a method for determination of trace uranium by the catalytic light mark. The catalytic light mark is composed of two parts of a DNA enzyme and a substrate DNA, a 3' end of a DNA enzyme sequence comprises four continuous guanine deoxynucleotides (G), the middle of a substrate DNA sequence has an adenine nucleotide (rA), and a 5' end of the substrate DNA is modified by a 4-methyl-6-carboxyl rhodamine (TAMRA) fluorescent dye. The DNA enzyme and the substrate DNA are in mixing hybridization to form the catalytic light mark, the catalytic light mark is added to a reaction system of a to-be-measured sample to undergo a reaction, the fluorescence intensity of uranyl nitrate ions with known concentration and the fluorescence intensity of the sample are determined, and the uranium content in the sample can be obtained through calculation. The method is a fast, accurate, high-sensitivity and high-selectivity method for determination of trace uranium.

The invention provides a method for constructing an artificial multi-enzyme system based on a lanthanide series nucleotide compound and a DNA (Deoxyribonucleic Acid) oriented immobilization technologyand belongs to the field of preparation of immobilized enzymes. The method comprises the following steps: firstly, preparing single-stranded DNA modified magnetic nanoparticles and complementary single-stranded DNA functionalized glucose oxidase; then mixing the magnetic nanoparticles with the enzyme; carrying out DNA complementary hybridization to realize the immobilization of the enzyme; commonly hatching the immobilized enzyme, cerium nitrate hexahydrate and disodium adenosine 5'-phosphate; carrying out self-assembling on a lanthanide series nucleotide coordinated polymer to realize the encapsulation of the immobilized enzyme and constructing the artificial multi-enzyme system. An encapsulation vector can realize the effect of protecting the immobilized enzyme and is also used as an analogue enzyme to form an enzyme cascade system with the immobilized natural enzyme. A preparation process of the artificial multi-enzyme system, provided by the invention, is simple, has moderate conditions and achieves high enzyme activity; the artificial multi-enzyme system is easy to separate from a reaction system and has excellent stability and reusability; meanwhile, the analogue enzyme is introduced and the cost is extremely reduced.

The present invention discloses a feed for treating Cyclemys trifasciata bacterial enterorrhagia, and a preparation method thereof, and belongs to the technical field of chelonia feed processing. The feed comprises the following raw materials: earthworm powder, Pomacea canaliculata powder, corn powder, sweet potato powder, edible salt, dimethyl-beta-propiothetin, betaine, phenylalanine, guanylic acid-adenylic acid, sodium diacetate, guanylic acid sodium, and a traditional Chinese medicine preparation, and drying, crushing, screening, mixing, granulating, drying, sterilization and other steps are performed to prepare the feed. According to the present invention, a variety of traditional Chinese medicine effects are integrated, effects of anti-bacterial effect, anti-inflammatory effect, body immune function enhancing and the like can be provided, the cure rate of the Cyclemys trifasciata bacterial enterorrhagia can be effectively improved, and the death rate can be reduced; the feed of the present invention has characteristics of reasonable preparation, rich nutrition, increase of the intake of the Cyclemys trifasciata having bacterial enterorrhagia, and growth rate improving; and the feed preparation method of the present invention has characteristics of simple operation and low production cost, and is easy to promote and apply.

Provided are diagnostic methods and kits including oligo and / or polynucleotides or derivatives, including as well antibodies determining whether a human subject is at risk of getting adverse drug reaction after statin therapy or whether the human subject is a high or low responder or a good a or bad metabolizer of statins. The diagnostic methods and kits including antibodies determining whether a human subject is at risk for a cardiovascular disease. Also provided are polymorphic sequences and other genes and isolated polynucleotides encoding a phenotype associated (PA) gene polypeptide useful in methods to identify therapeutic agents and useful for preparation of a medicament to treat cardiovascular disease or influence drug response.

Described herein are assays, kits and methods for treating mood disorders by testing for one or more polymorphisms in a specific group of genes and for analyzing the results of polymorphism testing; the genes included may converge in one or more signaling pathways, and may be epigenetic. The genes are included based on the relationships of the proteins encoded by the genes in the context of particular signaling pathways and provide a diagnostically relevant nexus. Also described herein are methods of presenting the data collected by the screen, including methods of delivering interpretive comments and / or treatment guidance based on the results of the genetic screening either individually or based on the genetic composition of particular clusters of genes which may be related to each other. Importantly, drugs which modulate these genetic disturbances are described for targeted therapeutic use based upon companion diagnostic method.

The structure of a soluble, functional fragment of human Apaf-1 protein having ADP bound thereto is disclosed. The invention includes such soluble, functional fragments of human Apaf-1 and other metazoan Apaf-1 homologs. Also included in the invention are methods of making such fragments and methods of using them, for example in screening methods to identify adenine nucleotide analogs and other compounds useful for alleviating or preventing disease conditions associated with inappropriate regulation of apoptosis.

The present teaching provides a fluorescent oligonucleotide probe having a high degree of design flexibility and wide applicability, as well as the use thereof. This is an oligonucleotide probe capable of forming a stem and loop, comprising at least one fluorophore located between adjacent nucleotides in the stem and is linked to a unit represented by Formula (1) and at least one quencher located at a site capable of pairing up with the at least one fluorophore located between the adjacent nucleotides in the stem and is linked to a unit represented by Formula (2). (In the formulae, X represents the fluorophore, Y represents the quencher, R1 represents an optionally substituted C2 or C3 alkylene chain, R2 represents an optionally substituted C0-2 alkylene chain, and Z represents a direct bond or linker.)

A composition comprising a novel Ca2+-activated, [ATP]i-sensitive nonspecific cation (NCCa-ATP) channel is described. The channel is found in mammalian neural cells and exhibits a different sensitivity to block by various adenine nucleotides, and is activated by submicromolar [Ca]i. The NCCa-ATP channel is activated under conditions of ATP depletion, which causes severe cell depolarization, followed by cell swelling. The NCCa-ATP channel is regulated by a sulfonylurea receptor and is inhibited by sulfonylurea compounds glibenclamide and tolbutamide. Methods employing compositions comprising the NCCa-ATP channel to screen for compounds that block the channel and the use of such antagonists as therapeutics in preventing brain swelling and damage are described. In addition, methods employing compositions comprising the Kir2.3 channel to screen for compounds that open the channel and the use of such antagonists as therapeutics in preventing brain swelling and damage are described.

A method of search for and identification of a eukaryotic IRES element active in cap-independent translation of RNA in eukaryotic cells is provided, comprising the following steps: i) screening eukaryotic mRNA sequences or corresponding DNA sequences for a potential IRES element having a block of nucleotides having: a) a length of at least 30 nucleotides; b) an adenine nucleotide content of at least 40 mol-%; and c) a pyrimidine nucleotide content of less than 40 mol-%; ii) inserting said potential IRES element into a linear dicistronic construct between an upstream gene and a downstream GUS reporter gene, whereby said potential IRES element is positioned for IRES-dependent translation of said downstream GUS gene and whereby said upstream gene is preceded by a stable hairpin structure to prevent IRES-independent translation of said genes; and iii) testing said potential IRES element for IRES-dependent translation of said GUS gene in a rabbit reticulocyte lysate or in a wheat germ extract in vitro translation assay, whereby GUS gene expression is quantitated preferably relative to a construct having a reference IRES element or a non-IRES element between said upstream gene and said GUS gene.