72 results about "Inosine monophosphate" patented technology

Flavivirus, rhabdovirus and paramyxovirus infections may be treated by administering an inhibitor of the enzyme dihydroorotate dehydrogenase such as 6-fluoro-2-(2′-fluoro-1,1′-biphenyl-4-yl)-3-methyl-4-quinolinearcarboxylic acid sodium salt (Brequinar). A synergistic effect can be obtained if an interferon such as interferon α2, interferon α8 or interferon β, or an inhibitor of a second enzyme selected from inosine monophosphate dehydrogenase, guanosine monophosphate synthetase, cytidine triphosphate synthetase and S-adenosylhomocysteine hydrolase, is also administered.

The invention discloses a spicy chicken essence seasoning and method for preparation, which is prepared from the following raw materials (by weight portion), chicken 5-15, gourmet powder 20-40, table salt 15-35, starch 5-15, starch gum 5-15, sugar 1-5, lycopene 0.5-2, ginger powder 0.5-3, Chinese prickly ash powder 0.2-3, fragrant onion 0.5-3, sodiam inosine monophosphate and/or sodium guanylate 0.2-5, chicken extract 0.5-5, hot pepper extract 1-5, and capsorubin 1-5.

The invention relates to a method for measuring the concentration of asymmetric dimethylarginine and a diagnostic reagent kit. The method comprises the following steps of: degrading asymmetric dimethylarginine (ADMA) by adopting dimethylarginine dimethylamino hydrolase to generate dimethylamine and citrulline; reacting the citrulline with adenosine triphosphate (ATP) and aspartate under the action of arginine succinate synthetase to generate adenosine monophosphate (AMP); generating inosine monophosphate (IMP) and ammonia by the AMP under the action of AMP deaminase; generating oxidization nicotinamide adenine dinucleotide (NAD) by the ammonia and deamination NAD under the action of NAD synzyme; and calculating the concentration of the ADMA by detecting the concentration of the NAD. The method and the reagent kit are accurate in results, and high in speed, repeatability and interference resistance, and are convenient to popularize and use.

The invention discloses an instant shark skin, which is produced by mixing, boiling and thickening the following raw materials with the following weight percents: 12 to 15 percent of fishy smell removed sharp skin, 3 to 5 percent of compounding material, and 80 to 85 percent of dripping seasoning. The compounding material is prepared by mushroom, black fungus, white fungus, veiled lady and dried scallop with a wet weight proportion of 3:3:6:6:2 after soaked. The dripping seasoning is prepared by the following dripping materials with the following weight percents and water: 10 to 15 percent of abalone juice, 0.3 to 0.5 percent of fresh chicken powder, 0.3 to 0.5 percent of white spirit, 0.1 to 0.3 percent of inosine monophosphate and guanosine monophosphate, 0.05 to 0.1 percent of salt, 0.01 to 0.05 percent of chicken extract, and 2 to 4 percent of starch. The invention has the advantages of convenient use, high nutritional value and good taste. The invention also discloses a preparation method of the instant sharp skin. The method has the advantage of good fishy smell removing effect; in addition, the compounding material and the dripping seasoning for the preparation of the sharp skin taste delicious and are rich in various nutrients.

The invention belongs to the technical field of a biosensor, and discloses an enzyme biosensor for detecting inosine monophosphate(IMP) and a preparation method and an application thereof. The lineardetection range of the enzyme biosensor is 0.313-210 microgram/L, and the detection limit is 0.238 microgram/L. The enzyme biosensor is formed by a reference electrode, a counter electrode and a modified electrode obtained by curing an IMP-sensitive substance recognition film on the surface of a working electrode. The substance recognition film is formed by a composite solution a, a double-enzymecomposite solution b formed by a 5'-nucleotidase solution and an xanthine oxidase solution by the volume ratio 1: 1, and a bovine serum albumin solution by the volume ratio of 1: 1: 1, wherein the composite solution a is composed of a MXene-Ti3C2Tx solution (T is selected from OH, O or F), a chitosan solution, a chloroauric acid solution and a chloroplatinic acid solution by the volume ratio of 12:1.2:1:1. The enzyme biosensor is simple, fast and accurate and high in sensitivity, and can be used for quantitative detection of inosine monophosphate(IMP) in food.

The invention discloses a Raman spectrum-based method for quickly detecting a umami substance inosine monophosphate in fresh fish flesh, relating to the technical field of aquatic product quality and safety detection. The method comprises the steps of 1, measuring a Raman spectrum of a inosine monophosphate standard substance; 2, acquiring a Raman spectrum of a fresh fish flesh sample; 3, measuring inosine monophosphate content by a chemical method; 4, performing Raman spectrum correction; 5, optimizing an inosine monophosphate characteristic peak; 6, calculating the area of the characteristic Raman peak; 7, building a detection model of the inosine monophosphate content. The umami substance inosine monophosphate in the fresh fish flesh is quickly detected by a microscopic confocal Raman spectra technique, and the Raman spectrum-based method for quickly detecting the umami substance inosine monophosphate in the fresh fish flesh which is effective, simple in operation and less in cost and time consumption is found; the detection method is simple, safe and efficient, can meet the industrialization production need and the requirements of consumers on product quality, and satisfies the need of evaluating the flavour and quality of fresh fish flesh products in a practical production and processing process.

The invention relates to an adenosine monophosphate (AMP) adenosine deaminase for synthesizing and metabolizing an inosine monophosphate from adenine nucleotide of Cordyceps sinensis(Berk.)Sacc. Hirsutella sinensis from 'Bailing' production strain, a gene coding the enzyme and application thereof. The AMP adenosine deaminase comprises the proteins shown in SEQ ID No. 1 and SEQ ID No. 2, and the coding gene of the AMP adenosine deaminase corresponds to the nucleotide sequence shown in SEQ ID No. 3 and SEQ ID No.4. According to the invention, the metabolizing way for synthesizing the inosine monophosphate from the adenine nucleotide is researched in detail from the principle, and the cloned DNA containing the nucleotide sequence provided by the invention can be transferred to an engineering strain by transduction, transformation, and combined transfer; the expression of the gene is biologically synthesized by regulating the inosine monophosphate, the host inosine monophosphate is endowed with high expressivity, an effective way is provided for increasing the yield of the inosine monophosphate, and the invention has a great application prospect.

The invention relates to a helicobacter pylori dominant antigen assembly based on CD4+T cell immunity and a preparation method. The dominant antigen assembly comprises the following three components and homologous protein of the three components: inosine monophosphate dehydrogenase, type II citrate synthase and urease B subunit, wherein the amino acid sequences are shown as SEQ ID NO.1, SEQ ID NO.2 and SEQ ID NO.3. The screened dominant antigen assembly based on CD4+T cell immunity has the obvious immune protection effect, has the protection effect superior to that of H.pylori holoprotein antigen, has strong capacity of scavenging helicobacter pylori, and causes extremely slight pathological injuries. The three immunity dominant antigens provided by the invention can induce the body to generate strong immune response reaction aiming at antigens, therefore, through the means of inducing the body to generate the response aiming at the immunoprotecive dominant antigens, or directly immunizing the body by adopting the protective antigens, the effective immune protection effect can be achieved on the helicobacter pylori infection, and the scheme can be used for the further study on the preventive and therapeutic polyvaccines of helicobacter pylori.

The invention discloses a method for increasing adenosine content in a paecilomyces hepiali fermentation mycelium. The method comprises the steps of strain activation, seed liquid preparation, fermentation with a fermentation tank and extraction, and a fermentation medium adopted in the fermentation step comprises 20-25 g/L of soybean cake powder, 10-15 g/L of glucose, 10-15 g/L of sucrose, 0.3-0.5 g/L of potassium dihydrogen phosphate, 0.2-0.4 g/L of MgSO4. 7H2O, 0.25 g/L of anhydrous calcium chloride, 0.4 g/L of soybean oil, 0.02-0.1 g/L of inosine monophosphate (IMP) and the balance of water. The IMP is added into the fermentation medium, so that the adenosine content in the paecilomyces hepiali mycelium powder can be increased, and the yield of the paecilomyces hepiali mycelium powdercan also be increased.

A pharmaceutical composition including an adjuvant effective amount of a protected inosine monophosphate (IMP) compound. The pharmaceutical composition includes the protected IMP compound alone, or in combination with vaccine agents with or without additional adjuvants. The pharmaceutical composition can be utilized as a vaccine composition or can be included with existing vaccine compositions in order to increase a specific T lymphocyte mediated immune response thereto. Various methods relating to the pharmaceutical composition and N the vaccine are described herein. The vaccines can be employed to prevent or treat infections. Additionally, the pharmaceutical compositions not only increase T-cell responses, but also confer, by pretreatment, non-specific protection against a variety of pathogens. This combination of actions is appropriate for enhancing defense against bioterrorism with organisms like smallpox or anthrax.

A method for improving the quality of mutton by using stems and leaves of liquorice comprises the following steps of (1) mixing the following ingredients in percentage by weight: 39%-40% of alfalfa, 19%-21% of withered and yellow liquorice stem and leaf grass meal, 29%-30% of corn, 4.0%-3.9% of cotton seed meal, 3.8%-3.9% of sunflower seed meal, 1.0%-1.1% of salt and 1.0%-1.1% of additives manually or by using a TMR (total mixed ration) machine or preparing the ingredients into total mixed ration particles on the basis of dry matters; and (2) feeding mutton sheep by using the daily ration mixed in the step (1) every morning and every evening at intervals of 10-11 hours. The feeding period is not shorter than 50 days, the content of the dry matters in the liquorice stem and leaf grass meal in the daily ration of the mutton sheep reaches 345-377 grams per day, and the mutton sheep freely eat the ration and freely drink water in the feeding period. By the method, the cholesterol content of the mutton can be reduced obviously, and the content of inosine monophosphate, amino acids and unsaturated fatty acids is increased, so that the quality of the mutton is improved.

The invention relates to an inosine monophosphate (IMP) dehydrogenase from 'Bailing' producing strain Cordyceps sinensis Hirsutella sinensis for participating in anabolism on IMP to obtain a xanthylic acid, a gene for encoding the dehydrogenase and application of the dehydrogenase. The IMP dehydrogenase comprises a protein shown by SEQ ID No.1 and a protein shown by SEQ ID No.2, and the coding gene of the protein shown by SEQ ID No.1 and the coding gene of the protein shown by SEQ ID No.2 respectively correspond to a nucleotide sequence shown by SEQ ID No.3 and a nucleotide sequence shown by SEQ ID No.4. According to the invention, the metabolic pathway of the IMP to synthetize the xanthylic acid is traversed from the principle; the cloning deoxyribonucleic acids (DNA) of the nucleotide sequences provided by the invention can be transferred into engineering bacteria through a transduction method, a transformation method and a conjugal transfer method; and through regulating the expression of the biosynthetic gene of the xanthylic acid, the high expressivity of a host xanthylic acid is endowed, and an effective way for increasing the yield of the xanthylic acid is provided. The IMP dehydrogenase has a great application prospect.

The invention relates to a 5'nucleotidase diagnosis kit using the technology of an enzyme colorimetric method and an ELISA method, a method for determining 5'nucleotidase activity concentration, and composition and components of a reagent, and belongs to the technical field of medical test and determination. The kit comprises the following main components: buffer solution, coenzyme, phosphate radical, inosine, inosine monophosphate dehydrogenase, and a stabilizer. The method comprises the following steps: mixing a sample and the reagent according to a certain volume ratio to perform a series of enzymic reaction, and then placing reactants under a UV/ visible light analyzer to test the ascending speed of absorbance at the position where the dominant wave length is 340nm to determine the activity concentration of 5'nucleotidase.

The invention discloses mycophenolic acid derivatives Penicacids A, B, C and their application in preparing immunosuppression medicaments. Penicacids A and B have a specific structure shown in (I), wherein, Penicacids A: R1=CH3, R2=OH; Penicacids B: R1=H, R2=OGlu. And Penicacids C has a specific structure shown in formula (II). Immunosuppressive activity evaluations on Penicacids A, B, and C find that Penicacids A, B and C have an obvious inhibiting effect on inosine monophosphate dehydrogenase (IMPDH), especially the compound Penicacids B which has good inhibitory activity. Therefore, Penicacids A, B, C can be used for preparing immunosuppression medicaments applicable in kidney, liver and other organ transplantations.

The invention relates to an adenosine deaminase diagnosis kit using the technology of an enzyme colorimetric method and an ELISA method, a method for determining adenosine deaminase activity concentration, and composition and components of a reagent, and belongs to the technical field of medical test and determination. The kit comprises the following main components: buffer solution, coenzyme, adenosine, adenosine triphosphate, inosine kinase, inosine monophosphate dehydrogenase and a stabilizer. The method for determining adenosine deaminase activity concentration comprises the following steps: mixing a sample and the reagent according to a certain volume ratio to perform a series of enzymic reaction, and then placing reactants under a UV/ visible light analyzer to test the ascending speed of absorbance at the position where the dominant wave length is 340nm to determine the activity concentration of adenosine deaminase.

The invention discloses a high-accuracy 5'-nucleotidase assay kit and relates to the technical field of bioassay. The kit comprises a reagent R1 and a reagent R2 which are independent from each other,wherein the reagent R1 is N-acetylpiperazine-N-2-ethanesulfonic acid, 4-aminoantipyrine, purine nucleoside phosphorylase, xanthine oxidase, peroxidase and sodium azide; and the reagent R2 is acetylpiperazine-N-2-ethanesulfonic acid, N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline, 5'-inosine monophosphate disodium salt and sodium azide. In the application process of the kit, by controlling the mass ratio of the components, the stability of the kit can be improved and assay accuracy and precision can be also improved, and in addition, the kit is applicable to fully automatic biochemical analyzers, is simple to operate, high in automation degree and applicable to wide clinical application.

The invention relates to a modified IMPDH polypeptide wherein the IMPDH polypeptide has a histidine tag and where the subdomain of the IMPDH polypeptide is modified so that the rate stability of the histidine-tagged, modified IMPDH polypeptide is maintained relative to the wild-type IMPDH polypeptide. The invention also relates to a method, nucleic acid molecules, vectors, and host cells for producing such a histidine-tagged, modified IMPDH polypeptide, and to kits comprising the histidine-tagged, modified IMPDH polypeptide.

The present disclosure relates to a novel protein variant having an activity of exporting 5′-inosine monophosphate, a microorganism comprising the protein variant, and a method for preparing 5′-inosine monophosphate using the microorganism.