A method for measuring the freshness of royal jelly

A method of determination, the technology of royal jelly, applied in the direction of testing food, measuring devices, instruments, etc., to achieve the effect of simple analysis method and high sensitivity

Inactive Publication Date: 2011-11-30
BEE RES INST CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, there is currently no reliable method for measuring the freshness of royal jelly

Method used

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  • A method for measuring the freshness of royal jelly
  • A method for measuring the freshness of royal jelly
  • A method for measuring the freshness of royal jelly

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] ATP, ADP, AMP, IMP, HxR, Hx, adenine and adenosine standard substance (purchased in Sigma company) are dissolved in ultrapure water, final concentration is 50ppm, measure with liquid chromatography, obtain standard liquid chromatography, see figure 1 .

[0031] Get newly harvested rapeseed royal jelly sample 0.5g, add 5ml 5% HClO 4 , mixed well, and centrifuged at 12000rpm for 10min; take the supernatant, add 700μL 6mol / L KOH and 50μL 2mol / L Na 2 CO 3 , after fully mixing, centrifuge at 12000rpm for 10min, filter the supernatant with a 0.45μm filter membrane, and 2 HPO 4 As the mobile phase, the content of each component is measured with a liquid chromatograph, and the obtained liquid chromatogram is shown in figure 2 .

[0032] Compare figure 1 and figure 2 Apparently, the characteristic peaks of Hx and adenine are not detected in the newly harvested royal jelly, indicating that Hx and adenine are hardly contained, while the characteristic peaks of AMP and IMP...

Embodiment 2

[0037] Get each 0.5g of rapeseed royal jelly samples stored at -18°C for 60 days, 4°C for 60 days, 16°C for 60 days and 28°C for 60 days, and add 5ml of 5% HClO 4 , mixed thoroughly, and centrifuged at 12000rpm for 10min. Take the supernatant, add 700 μL 6mol / L KOH and 50 μL 2mol / L Na 2 CO 3 , after fully mixing, centrifuge at 12000rpm for 10min, filter the supernatant with a 0.45μm filter membrane, and 2 HPO 4 As the mobile phase, it is determined with a liquid chromatograph (see image 3 , 4 , 5 and 6).

[0038]It can be seen that with the increase of storage temperature, the contents of IMP, ATP, ADP, and AMP of royal jelly decreased gradually, while the contents of adenine and adenosine gradually increased, and the contents of Hypoxanthine (Hx) and Inosine (HxR) decreased first, and then remained stable . It is basically consistent with the aforementioned degradation mechanism of the inventor. The ATP content of royal jelly stored at 28°C for 60 days was 0, indicat...

Embodiment 3

[0044] Get each 0.5g of camellia royal jelly samples stored at 4°C for 15 days, 30 days, 60 days and 90 days, adopt the method of Example 1, add 5ml 5% HClO 4 , mixed thoroughly, and centrifuged at 12000rpm for 10min. Take the supernatant, add 700 μL 6mol / L KOH and 50 μL 2mol / L Na 2 CO 3 , after fully mixing, centrifuge at 12000rpm for 10min, filter the supernatant with a 0.45μm filter membrane, and 2 HPO 4 As the mobile phase, it was determined with a liquid chromatograph. The contents of ATP and its degradation products in royal jelly were calculated, and the results are shown in Table 2.

[0045] Table 2 Degradation product content (mg / kg) and F value of camellia royal jelly stored at 4°C for different time

[0046]

[0047] It can be seen that with the increase of storage time, the contents of ATP, ADP, AMP, and IMP of royal jelly decreased gradually, while the contents of adenine and adenosine gradually increased, and the contents of Hypoxanthine (Hx) and Inosine ...

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Abstract

The invention relates to a method for detecting the freshness of royal jelly. The specific determination method is: Quantitative determination of adenosine triphosphate (ATP), adenosine diphosphate (ADP), adenosine monophosphate (AMP), inosinic acid (IMP), secondary Xanthine nucleoside (HxR), hypoxanthine (Hx), adenine (adenine) and adenosine (adenosine); calculate the cumulative amount of adenine, adenosine, HxR and Hx and the above 8 substances (ATP and nucleic acid The ratio (F value) of the quality sum of degradation products); Utilize the F value size of royal jelly to detect the freshness of royal jelly, judge the quality of royal jelly. Compared with the existing method for judging the quality of royal jelly, the method can judge the quality and freshness of royal jelly more accurately and sensitively, can improve the current quality standard of royal jelly, and effectively monitor the quality of royal jelly.

Description

technical field [0001] The invention relates to a food quality detection method, in particular to a method for measuring the freshness of royal jelly. Background technique [0002] Royal jelly is secreted by the hypopharyngeal glands and mandibular glands of the feeding bees, and is used to feed the queen bee and 1-3 day-old bee larvae. It plays an important role in the hierarchical differentiation of bees. Royal jelly is rich in biologically active substances, and has nutritional and health effects on the human body such as anti-fatigue, anti-bacterial and anti-inflammatory, anti-tumor, lowering blood pressure, and promoting growth. It has high application value in the fields of health food, medicine, and cosmetics. The excellent nutritional and health effects make royal jelly and its products popular all over the world. With the improvement of domestic people's living standards and the growth of health care needs, the domestic royal jelly market is gradually growing. [...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/02G01N30/86G01N21/00
Inventor 吴黎明薛晓锋赵静李熠彭文君胡福良
Owner BEE RES INST CHINESE ACAD OF AGRI SCI
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