The invention discloses a method for detecting a Simotang preparation. The method is characterized in that:
high performance liquid chromatography detection is carried out on a
test sample of the Simotang preparation, and a chromatographic condition is selected from one of the following two
modes: a mode 1 is characterized in that: the
chromatographic column is a strongly
cationic exchange chromatographic column, the mobile phase comprises
methanol and an
aqueous solution with a
volume percentage of 0.2% and a pH value of 3.8 according to a volume ratio of 40-80:60-20, and the detection
wavelength is 215nm; and a mode 2 is characterized in that: the
chromatographic column is an octadecylsilane bonded
silica gel column, the mobile phase comprises
methanol and an
aqueous solution of SDS and with a
mass percentage of 0.1% according to a volume ratio of 50:50, the flow velocity is in a gradient mode, and the detection
wavelength is 230nm. The method of the invention, which allows three
alkaloid components of arecolidine,
synephrine and norisobolidine in the Simotang preparation to be simultaneously determined, the resolution of chromatographic peaks, a theoretic
stage number, a symmetry factor, and the peak purity obtained through the method to be good, and a
linear relation between the concentration and the
peak area to be good in a large concentration range, has the advantages of excellent precision,
repeatability and accuracy.