The invention relates to an anisotropism evaluation method for oil and gas generation quantity of shale. The method includes the following steps that firstly, different depth points on a section of the shale are densely sampled, basic parameter values are obtained through Rock-Eval pyrolysis detection, and organic matter types are determined; secondly, a conceptual model is established, and contents of organic carbon of different types are calculated; thirdly, a simulated experiment is designed, and a heavy hydrocarbon oil yield and natural gas yield evolvement model is established; fourthly, the total quantity of hydrocarbon generated and discharged by the different depth points of the shale and the quantity of heavy oil, light oil and gas generated and discharged by the different depth points of the shale are calculated, and the anisotropism of the oil bearing possibility and gas bearing possibility of the shale is evaluated. The models are established on the basis of a large number of sample data points of the section, anisotropism evaluation can be carried out on oil and gas resources of the shale, under the situation that samples are limited, the method is still suitable in combination with logging information, and therefore the method has a great significance in quantitatively representing the anisotropism of the oil bearing possibility and gas bearing possibility of the shale and accurately evaluating unconventional shale oil and gas resources.