Method for preparing real substance sample for authentication of propolis liquid phase fingerprint

Abstract

The invention relates to a method for preparing a real substance sample for authentication of a propolis liquid phase fingerprint. At present, the mature method for authenticating poplar gum and propolis is unavailable. The method comprises the processes of preparing a propolis substance sample and a poplar gum substance sample. The process of preparing the propolis substance sample comprises thefollowing steps of: soaking the propolis in 95 percent edible ethanol; drying the refined propolis liquid in vacuum in a water bath at the temperature of 80 to 95 DEG C; and mixing the crushed refined propolis and an excipient, and sieving the mixture with an 80-mesh sieve to obtain the propolis substance sample. The process of preparing the poplar gum substance sample comprises the following steps of: heating polar-water mixed liquid to over 90 DEG C; pressing the liquid to obtain a polar liquid; after cooking the polar liquid, soaking the polar liquid in the 95 percent edible ethanol; drying the refined polar gum liquid in vacuum in the water bath at the temperature of 80 to 95 DEG C; and mixing the crushed refined polar gum liquid and the excipient, and sieving the mixture with the 80-mesh sieve to obtain the poplar gum substance sample. The method has the advantages of reasonable and scientific preparation, strong operability and application to effective authentication of the false propolis prepared from polar sprouts.

Description

technical field The invention relates to a method for preparing a physical standard sample, in particular to a method for preparing a physical standard sample used for the authenticity identification of propolis liquid fingerprints. The standard sample is mainly used to identify poplar gum and propolis, and to identify the authenticity of propolis by comparing the consistency of each characteristic chromatographic peak of the liquid phase fingerprint of the test sample and the physical standard sample. Background technique At present, the phenomenon of propolis production and adulteration is intensifying. Due to the lagging of the existing propolis standards, it is difficult to identify the fake propolis made from poplar buds through the human senses, and the quality indicators of this kind of fake propolis fully comply with the current national standards. That is to say, it is difficult to distinguish the authenticity of propolis products with the current national standards...

AI Technical Summary

Problems solved by technology

In summary, there is no mature method for distinguishing poplar gum and propolis at present, and there is no propolis physical label designed specifically for the Chinese patent whose publication date is January 11, 2006, and the publication number is CN1719247. sample and poplar gum physical standard sample, thus bringing certain obstacles to the widespread popularization of this patent, and also hindering the better development of methods for identifying poplar gum and propolis

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