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Biocompatible metal organic framework material glutamic acid-zinc as well as preparation and application thereof

A metal-organic framework and biocompatible technology, applied in the field of biocompatible metal-organic framework material glutamic acid-zinc and its preparation and application, can solve the problems of slow speed and low solubility, and achieve mild reaction and fast generation speed. , the effect of reducing the reaction temperature

Inactive Publication Date: 2016-06-08
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Glutamic acid has low solubility in cold water and organic solvents, and it is a weak acid that is not easily ionized into -COO - , resulting in a Zn 2+ Generate Zn(L-Glu)(H 2 O)]H 2 O is very slow

Method used

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  • Biocompatible metal organic framework material glutamic acid-zinc as well as preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] (1) 15 mL of disodium glutamate aqueous solution containing 1.8 mmol was added dropwise to 45 mL containing 3 mmol of Zn(NO 3 ) 2 in methanol (disodium glutamate and Zn(NO 3 ) 2 The molar ratio of 3:5; the volume ratio of disodium glutamate aqueous solution to methanol is 1:3, containing Zn(NO 3 ) 2 Zn(NO 3 ) 2 The concentration is 0.05mol / L), obtain white precipitate immediately, centrifuge and freeze-dry after washing, obtain biocompatible metal-organic framework material glutamic acid-zinc carrier material (Zn(L-Glu)(H 2 O)]H 2 O)( figure 1 );

[0042] (2) Aspergillus niger lipase is dispersed in the phosphate buffer solution that pH is 8, obtains enzyme solution (20mg / mL);

[0043] (3) At 25°C, add 2mg of EDC·HCl to 1mL enzyme solution and stir at 150rpm for 1h to obtain mixed solution A;

[0044] (4) Add 2.4 mg of NHS to the mixed solution A obtained in step (3) at 25° C. and continue stirring for 1 hour to obtain mixed solution B;

[0045](5) Take 44 mg...

Embodiment 2

[0047] (1) 15mL aqueous solution containing 1.5mmol disodium glutamate was added dropwise to 45mL containing 3mmol Zn(NO 3 ) 2 in methanol (disodium glutamate and Zn(NO 3 ) 2 The molar ratio of 1:2; the volume ratio of disodium glutamate aqueous solution to methanol is 1:3, containing Zn(NO 3 ) 2 Zn(NO 3 ) 2 The concentration is 0.05mol / L), obtain white precipitate immediately, centrifuge and freeze-dry after washing, obtain biocompatible metal-organic framework material glutamic acid-zinc carrier material (Zn(L-Glu)(H 2 O)]H 2 O);

[0048] (2) Aspergillus niger lipase is dispersed in the phosphate buffer solution that pH is 8, obtains enzyme solution (20mg / mL);

[0049] (3) At 25°C, add 2mg of EDC·HCl to 1mL enzyme solution and stir at 150rpm for 1h to obtain mixed solution A;

[0050] (4) Add 2.4 mg of NHS to the mixed solution A obtained in step (3) at 25°C and continue stirring at 150 rpm for 1 hour to obtain mixed solution B;

[0051] (5) Take 44 mg of the bioco...

Embodiment 3

[0053] (1) 15mL aqueous solution containing 1.0mmol disodium glutamate was added dropwise to 45mL containing 3mmol Zn(NO 3 ) 2 in methanol (disodium glutamate and Zn(NO 3 ) 2 The molar ratio of 1:3; the volume ratio of disodium glutamate aqueous solution to methanol is 1:3, containing Zn(NO 3 ) 2 Zn(NO 3 ) 2 The concentration is 0.05mol / L), obtain white precipitate immediately, centrifuge and freeze-dry after washing, obtain biocompatible metal-organic framework material glutamic acid-zinc carrier material (Zn(L-Glu)(H 2 O)]H 2 O);

[0054] (2) Aspergillus niger lipase is dispersed in the phosphate buffer solution that pH is 8, obtains enzyme solution (20mg / mL);

[0055] (3) At 25°C, add 2mg of EDC·HCl to 1mL enzyme solution and stir at 150rpm for 1h to obtain mixed solution A;

[0056] (4) Add 2.4 mg of NHS to the mixed solution A obtained in step (3) at 25°C and continue stirring at 150 rpm for 1 hour to obtain mixed solution B;

[0057] (5) Take 44 mg of the bioco...

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Abstract

The invention belongs to the technical field of new materials and particularly relates to biocompatible metal organic framework material glutamic acid-zinc as well as preparation and an application thereof. The biocompatible metal organic framework material glutamic acid-zinc is obtained by dropwise adding a disodium glutamate to a Zn(NO3)2 methanol solution. The method has the characteristics that the production speed of glutamic acid-zinc crystals is high, the preparation condition is mild, the prepared product is good in crystal form and the like, can be applied to a series of amino-rich enzymes such as lipase, protease, epoxide and the like and has the potential of being used as a protein drug carrier. The product is added to an Aspergillus niger lipase phosphate buffer modified with EDC or EDC*HCl and NHS for oscillation immobilization, and immobilized Aspergillus niger lipase is obtained. The method is high in immobilization efficiency, high in enzyme activity recovery rate, convenient to operate, mild in condition and the like.

Description

technical field [0001] The invention belongs to the technical field of new materials, and in particular relates to a biocompatible metal organic framework material glutamic acid-zinc and its preparation and application. Background technique [0002] Enzyme immobilization on metal-organic frameworks (MOFs) is a research hotspot at home and abroad because of its unique and diverse topology, porosity, and physical properties. Metal-organic framework materials have many properties such as porosity, large specific surface area, and multiple metal sites, and have good application prospects in the fields of gas storage, molecular separation, catalysis, and drug sustained release. At present, encapsulation and physical adsorption methods are commonly used to immobilize enzymes on metal-organic framework materials to immobilize enzymes on mesoporous carriers, and most of the organic ligands used are traditional organic ligands. In-depth research requires MOF to be constructed from b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C227/18C07C229/76C12N11/02
CPCC12N9/20C12N11/02C12Y301/01003C07C227/18C07C229/76
Inventor 娄文勇夏高辉曹诗林宗敏华
Owner SOUTH CHINA UNIV OF TECH
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