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55results about How to "High enzyme loading" patented technology
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Nanofibers with high enzyme loading for highly sensitive biosensors
ActiveUS20110229916A1High areaIncrease flexibilityBioreactor/fermenter combinationsBiological substance pretreatmentsFiberEnzyme system
The invention provides high enzyme loading nanofibers and processes utilized in their fabrication, the nanofibers suitable for use as a new class of highly sensitive and stable biosensors capable of monitoring glucose at low levels. The biosensors, comprising nanofiber enzyme materials fabricated from organic solvent-based polymer-enzyme systems, can be used effectively in non-invasive transdermal biosensing applications.
Owner:THE UNIVERSITY OF AKRON
Immobilization of enzyme on a fibrous matrix
ActiveUS7166451B1The process is simple and clearHigh enzyme loadingOn/in organic carrierFermentationFiberCross-link
A multilayer enzyme immobilization process is provided comprising adsorbing a polyethyleneimine (PEI) solution in a fibrous matrix, and adding an enzyme to the fibrous matrix, which comprises a plurality of fibrils. The process further comprises forming at least two layers of PEI-enzyme aggregates on the fibrils, and cross-linking the multilayer PEI-enzyme aggregates. The process can further comprise washing the fibrils containing the cross-linked PEI-enzyme aggregates with distilled water and acetic acid buffer subsequent to cross-linking. However, the PEI-containing matrix is not washed prior to the addition of enzyme. The enzyme can be β-galactosidase and the fibrous matrix can be cotton cloth. The multilayer immobilized enzyme can be employed in a biocatalyst reactor for production of galacto-oligosaccharides from lactose and the hydrolysis of lactose to glucose and galactose.
Owner:THE OHIO STATE UNIV RES FOUND
Immobilization of enzyme on a fibrous matrix
ActiveUS20070111289A1High enzyme loadingEasy loadingBioreactor/fermenter combinationsBiological substance pretreatmentsCross-linkFiber
A multilayer enzyme immobilization process is provided comprising adsorbing a polyethyleneimine (PEI) solution in a fibrous matrix, and adding an enzyme to the fibrous matrix, which comprises a plurality of fibrils. The process further comprises forming at least two layers of PEI-enzyme aggregates on the fibrils, and cross-linking the multilayer PEI-enzyme aggregates. The process can further comprise washing the fibrils containing the cross-linked PEI-enzyme aggregates with distilled water and acetic acid buffer subsequent to cross-linking. However, the PEI-containing matrix is not washed prior to the addition of enzyme. The enzyme can be β-galactosidase and the fibrous matrix can be cotton cloth. The multilayer immobilized enzyme can be employed in a biocatalyst reactor for production of galacto-oligosaccharides from lactose and the hydrolysis of lactose to glucose and galactose.
Owner:THE OHIO STATE UNIV RES FOUND
Method for preparing immobilized enzyme biological catalyst
InactiveCN101235368AGood Enzyme DispersionHigh enzyme loadingOn/in organic carrierWastewaterSilicon oxide
The invention designs a process for preparation, which utilizes silica-based mesoporous molecular sieves and calcium alginate to fix enzyme biocatalyst, and belongs to the technical field of fixing enzyme biocatalyst preparation technique. The process for preparation comprises utilizing silica-based mesoporous molecular sieves MCM-41 to be carrying agent, fixing biocatalyst molecule in meso-porous pore path of silicon oxide carrier, secondarily fixing immobilized enzyme biocatalyst which is prepared in calcium alginate gel. The process for preparation of the invention adopts a physical adsorption embedding method and a collosol-gel method. The fix enzyme biocatalyst of the invention has better enzyme dispersibility and higher enzymes loading, and has higher enzymatic activity recovery ratio and repeated utilization ratio. The invention can fix various enzymes such as horse radish peroxidase, proteinase, lipase and the like. The fix enzyme biocatalyst of the invention can be used to process waste water which contains hydroxybenzene and decolorize, which has higher catalytic activity.
Owner:SHANGHAI UNIV
Preparation method and application of macroporous polyacryamide resin
InactiveCN101899130AEasy to prepareLow reaction temperatureOn/in organic carrierReaction temperaturePolyacrylamide
The invention relates to a preparation method of macroporous polyacryamide resin. The method comprises the following steps of: preparing an aqueous phase, preparing an oil phase, preparing the macroporous polyacryamide resin and carrying out after-treatment on products. The preparation method of the macroporous polyacryamide resin has simplicity and low reaction temperature, and the prepared macroporous polyacryamide resin has macroporous structure from the surface to the interior, uniform and dense aperture, relatively larger specific surface area and strong water absorbability and adsorbability, can be used as a carrier of an immobilized enzyme and can greatly improve the capacity of the enzyme.
Owner:SHAANXI NORMAL UNIV
Novel immobilized enzyme preparation, and preparation method and application thereof
InactiveCN106520747AImprove stabilityEasy to fixOn/in organic carrierPeroxidaseMetal-organic framework
The invention belongs to the field of biological catalysis, and particularly relates to a novel immobilized enzyme preparation, and a preparation method and application thereof. The preparation method of the novel immobilized enzyme preparation comprises the following steps: combining nano magnetic chitosan microspheres and a metal-organic framework material having so many properties such as multiple pores, large specific area, multiple metal sites and the like through graft copolymerization to obtain a nano magnetic chitosan-metal organic framework material having a core-shell structure with a porous network structure; and mixing the nano magnetic chitosan-metal organic framework material used as a carrier and an enzyme solution, and immobilizing at 0-25 DEG C for 1-12 hours to obtain the novel immobilized enzyme preparation. The method can immobilize enzymes such as lipase, proteinase, peroxidase and the like, and has the advantages of low cost, high immobilization efficiency, favorable enzyme stability, high enzyme loading amount and high enzyme recovery rate; no bifunctional reagent is used in the immobilization process; and the method is simple to operate, low in cost and mild in reaction.
Owner:GUANGZHOU KAIYAO ASSET MANAGEMENT CO LTD
Transaminase immobilization method
ActiveCN104774830ARich in holesIncrease the areaOn/in organic carrierCross-linkPolyvinyl alcohol sponge
The invention provides a transaminase immobilization method. The method comprises the following steps: oxidizing a hydroxyl group on polyvinyl alcohol sponge, adding transaminase to convert a carbonyl group into an amino group, flocculating, and carrying out cross-linking immobilization by using polyaldehyde to obtain immobilized transaminase. The immobilization method has the advantages of high enzyme activity unit and enzyme purification.
Owner:四川百特芳华医药科技有限公司
Soybean epoxy compound hydrolase preparation and immobilization method
The invention belongs to the technical field of materials and discloses a soybean epoxy compound hydrolase immobilization method and an enzyme preparation. The soybean epoxy compound hydrolase immobilization method comprises the following steps: dispersing soybean epoxy compound hydrolase in a buffer solution with the pH of 5-9 to prepare an enzyme solution, mixing the enzyme solution with UiO-66-NH2 nano crystals to obtain a mixture, adding a saturate ammonium sulfate solution in the mixture at 0-35 DEG C to enable the final saturability of ammonium sulfate to be 80%, stirring and mixing for 1-60 min to obtain a mixed turbid liquid, mixing the mixed turbid liquid with a glutaraldehyde solution, reacting for 1-200 min in a stirring manner, and freeze-drying to obtain a soybean epoxy compound hydrolase preparation. According to the invention, a simple sedimentation crosslinking technology is adopted, the cost is lower, the reaction is moderate, the enzyme activity can be kept to a greater degree, the enzyme loading amount of a carrier can be increased, the enzyme activity recovery rate can be increased, and the immobilization effect is excellent.
Owner:SOUTH CHINA UNIV OF TECH
Preparation method and application of polyisophthaloyl metaphenylene diamine-polyacrylonitrile composite nanofiber film
InactiveCN103726233AEasy to makeLarge specific surface areaFilament/thread formingNon-woven fabricsPolymer scienceAcrylonitrile
The invention relates to a method for preparing a nanofiber film by blending polyisophthaloyl metaphenylene diamine and polyacrylonitrile and application of the nanofiber film. The preparation method concretely includes the steps that a polyisophthaloyl metaphenylene diamine polymer solution with the 5-8% mass percent is prepared; a polyacrylonitrile solution with the 5-8% mass percent is prepared; the two solutions are blended with the mass ratio of polyisophthaloyl metaphenylene diamine :polyacrylonitrile=3:1-1:5, and the polyisophthaloyl metaphenylene diamine-polyacrylonitrile composite nanofiber film is prepared through a electrostatic spinning method and serves as an enzyme immobilization carrier; the enzyme immobilization carrier is modified through a sodium hydroxide / ethylenediamine surface reaction method; enzymes are immobilized on the nanofiber film with reactive amino groups and reactive carboxy groups on the surface. According to carrier preparation and the enzyme immobilization process, the preparation method and the application of the polyisophthaloyl metaphenylene diamine-polyacrylonitrile composite nanofiber film have the advantages that operation is easy, enzyme loading, catalytic activity and mechanical strength of the carrier are high, and the polyisophthaloyl metaphenylene diamine-polyacrylonitrile composite nanofiber film can be used repeatedly.
Owner:TONGJI UNIV
Phospholipid modified acrylon nano fiber composite film and its preparation and immobilized enzyme thereof
InactiveCN1837266ASufficient sourceSimple production processWet spinning methodsOn/in organic carrierFiberComposite film
The invention discloses a phosphatide modified acrylic nanometer fibre composite film and preparing and immobilized enzyme method, which comprises the following steps: taking dimethyl sulfoxide, dimethyl formamide or dimethylacetamide as solvent; preparing acrylon copolymer with phosphatide group through electrostatic spinning method as the carrier of immobilized enzyme; immobilizing the enzyme on the nanometer fiber composite film through physical adsorptive method. The invention applies bionic property of high specific surface area, high gap rate and phosphatide property, which produces high enzyme carrying, specific activity and catalyzing rate immobilizing enzyme phosphatide modified acrylic nanometer fibre composite film.
Owner:ZHEJIANG UNIV
Grease catalysis separation biphasic enzyme-film bioreactor and its preparation and application
InactiveCN101265448ANot easy to fall offIncrease the areaFibre typesMonocomponent synthetic polymer artificial filamentFiberLipid formation
The invention relates to a grease catalyzing and separating biphasic enzyme-membrane reactor and a method for preparing and using hydrolyzed edible glyceride. The method comprises the steps of filling polyphosphazene superfine fiber complex film into a mould with an inner and an outer cylinders to form a roll membrane device; sequentially injecting activating agent solution and lipase solution into the membrane device, and causing the lipase to be chemically fixed on the surface of the superfine fiber film to obtain an enzyme-membrane reactor; and injecting edible glyceride and phosphate buffer respectively into the inner side and the outer side of the superfine fiber film to conduct hydrolysis reaction on the surface of the superfine fiber surface. The reactor can be used for catalyzing the hydrolysis of the glyceride, and for producing diglyceride or monoglyceride edible oil and extracting functional lipid by treating lard or fish oil. The glyceride hydrolyzate accounts for more than 85%. The grease catalyzing and separating biphasic enzyme-membrane reactor is designed by combining efficient biocatalysis and complex film separation, and has the advantages of repeatable use, realized continuous efficient production, reduced cost, saved resource and good development prospect.
Owner:ZHEJIANG UNIV
Preparation method for polyacrylonitrile immobilized enzyme
InactiveCN103255124AEasy to makeEnzyme immobilization is simpleBiochemical fibre treatmentFibre typesPolymer scienceAcrylonitrile
The invention relates to a preparation method for polyacrylonitrile nanofiber membrane immobilized enzyme. The preparation method comprises the following steps of dissolving a polyacrylonitrile polymer into N,N-dimethylformamide and preparing a polyacrylonitrile nanofiber membrane through electrostatic spinning; then soaking the polyacrylonitrile nanofiber membrane into an absolute alcohol solution, aerating hydrogen chloride gas for 10-30 minutes, and fixing enzyme into the nanofiber membrane through chemical bonding. According to the preparation method, high voidage and high mechanical strength of the polyacrylonitrile nanofiber membrane are utilized for preparation of the nanofiber membrane immobilized enzyme which has high enzyme content, is easy to recycle from a reaction system and can be used repeatedly. The immobilized zymophore can be used for increasing the use ratio of enzyme and lowering the production cost, and has the advantage of simple preparation processes, and the like.
Owner:TONGJI UNIV
Preparation method of immobilized lipase used for grease hydrolysis
InactiveCN101538566AHigh enzyme loadingHigh retention activityOn/in organic carrierFermentationCellulosePotassium hydroxide
The invention discloses a preparation method of immobilized lipase used for grease hydrolysis, comprising the following steps of: dissolving cellulose in a dimethyl acetamide / acetone mixed solution to prepare a uniform solution which is rested for removing bubbles to obtain a spinning solution; then preparing cellulose superfine fibrous membrane by electrostatic spinning; placing the cellulose superfine fibrous membrane in the ethanol solution of potassium hydroxide for hydrolysis to generate hydroxyl group which is activated after being washed for a plurality of times by de-ionized water; and then placing the activated cellulose superfine fibrous membrane in an enzyme solution, and obtaining the immobilized lipase of the cellulose superfine fibrous membrane after reaction for a plurality of hours. The prepared immobilized lipase can be used for grease hydrolysis. The method adopts the cellulose superfine fibrous membrane and has the advantages of simple and fast technology, good repeatability, low cost and safe operation and being environment-friendly and suitable for large-scale industrialized production, furthermore, the prepared immobilized lipase has large enzyme-loading capacity, and catalytic efficiency for grease hydrolysis is high.
Owner:ZHEJIANG UNIV
Preparation method of block polymer immobilized enzyme
InactiveCN103255125ALarge specific surface areaHigh porosityBiochemical fibre treatmentFibre typesFiberSpinning
The invention relates to a preparation method of a block polymer immobilized enzyme. The method comprises the following steps of dissolving polymethyl methacrylate-ethyl acrylate block polymer into N,N-dimethylformamide and tetrahydrofuran which are in a certain mass ratio; preparing polymethyl methacrylate-ethyl acrylate nano fiber film through an electrostatic spinning method; with the polymethyl methacrylate- ethyl acrylate nano fiber film as an immobilized carrier of the enzyme, and fixing the enzyme onto the nano fiber film through a method for ammonificating polyethylenimine and activating glutaraldehyde. By utilizing the high specific surface area and high void ratio of the nano fiber film, the nano fiber film immobilized enzyme with high enzyme carrying capacity and high catalyzing efficiency can be obtained. The immobilized enzyme has the advantages that the immobilized enzyme is easily recovered from a reaction system and can be reutilized for multiple times, the utilization rate of the enzyme can be increased, the production cost can be reduced, and the preparation process is simple.
Owner:TONGJI UNIV
Method of preparing linoleate isomerase micro-capsules on the basis of polyelectrolyte layer-by-layer self-assembly technology
InactiveCN104974996AControllable wall thicknessIncrease the areaOn/in organic carrierHalf-lifeAmorphous calcium carbonate
The invention provides a method of preparing linoleate isomerase micro-capsules on the basis of a polyelectrolyte layer-by-layer self-assembly technology and can solve a problem that free linoleate isomerase is poor in tolerance, is poor in stability and cannot be recycled in industrial production. The method of the invention includes the following steps: with an amorphous calcium carbonate crystal, which is obtained through co-precipitation of calcium carbonate and the linoleate isomerase, as a template and with polyallylamine hydrochloride and sodium polystyrenesulfonate as coating materials, preparing the linoleate isomerase micro-capsules through the polyelectrolyte layer-by-layer self-assembly technology. The micro-capsule is high in activity, excellent in stability and long in half life.
Owner:INNER MONGOLIA AGRICULTURAL UNIVERSITY
Biocompatible metal organic framework material glutamic acid-zinc as well as preparation and application thereof
InactiveCN105646253ALow costGenerate fastHydrolasesOrganic compound preparationMetal-organic frameworkPhosphoric acid
The invention belongs to the technical field of new materials and particularly relates to biocompatible metal organic framework material glutamic acid-zinc as well as preparation and an application thereof. The biocompatible metal organic framework material glutamic acid-zinc is obtained by dropwise adding a disodium glutamate to a Zn(NO3)2 methanol solution. The method has the characteristics that the production speed of glutamic acid-zinc crystals is high, the preparation condition is mild, the prepared product is good in crystal form and the like, can be applied to a series of amino-rich enzymes such as lipase, protease, epoxide and the like and has the potential of being used as a protein drug carrier. The product is added to an Aspergillus niger lipase phosphate buffer modified with EDC or EDC*HCl and NHS for oscillation immobilization, and immobilized Aspergillus niger lipase is obtained. The method is high in immobilization efficiency, high in enzyme activity recovery rate, convenient to operate, mild in condition and the like.
Owner:SOUTH CHINA UNIV OF TECH
An enzyme biofuel cell for generating electricity from trehalose produced by Escherichia coli in sewage
ActiveCN109148924AExpand the scope ofIncrease enzyme load and activityCell electrodesBiochemical fuel cellsEnzyme electrodeChemistry
The invention discloses an enzyme biofuel cell for generating electricity from trehalose produced by Escherichia coli in sewage. The invention successfully prepares a two-chamber enzyme biofuel cell capable of generating electricity by using trehalose from sewage by immobilizing engineering Escherichia coli containing trehalose hydrolase TreA and glucose oxidase GOx after whole-cell permeability treatment on an anode carbon felt and immobilizing bilirubin oxidase on a cathode carbon felt. The fuel cell assembled by the invention has a maximum output power of up to 16.2. M u.W / cm2, and the output current density of the fuel cell can still be maintained at 72% of the initial maximum output value after 60 hours of continuous power generation under the condition that the raw material is sufficient. The method for preparing the fuel cell of the invention is simple and low in cost, and the reaction is carried out at room temperature, and the prepare enzyme electrode has good catalytic performance and good application prospect.
Owner:NANJING UNIV OF TECH
Method for producing high fructose syrup by immobilized glucose isomerase
InactiveCN106047963ANo effect on activityEasy to operateOxidoreductasesIsomerasesFiltration membraneEnzyme membrane
The invention discloses a method for producing high fructose syrup by immobilized glucose isomerase. The method comprises the following steps: immobilizing glucose isomerase in an ultra-filtration membrane with an asymmetric structure in a filtering mode to prepare a membrane bioreactor of the immobilized glucose isomerase; performing reaction on the glucose solution in batches or continuously by utilizing the membrane bioreactor to produce the high fructose syrup. According to the method, an enzyme catalysis process and a membrane separating process are integrated, so that the production process is simplified, and the activity and the stability of an enzyme are improved; meanwhile, an enzyme membrane reactor can be used repeatedly, solvent cleaning and batchwise feeding are avoided, the development direction of green chemistry is met, continuous production is realized, the production efficiency is improved, and reference is provided for industrial production of fructose.
Owner:ZHEJIANG GONGSHANG UNIVERSITY
Efficient immobilized enzyme column and preparation method and application thereof
InactiveCN110257367AImprove adsorption capacityFacilitate absorption and infiltrationOn/in organic carrierOn/in inorganic carrierPorous substrateLactase
The invention discloses an efficient immobilized enzyme column and a preparation method and application thereof. The efficient immobilized enzyme column comprises a porous base material and active biological enzymes, a porous structure of the porous base material allows simultaneous distribution of large holes, medium holes and small holes, the large holes, the medium holes and the small holes are communicated to form three-dimensional through structures, and the active biological enzymes are fixed to surfaces of the three-dimensional through structures according to a glutaraldehyde crosslinking method. The porous base material is prepared by loading of agarose gel on porous carbon material powder and is filled in situ and condensed in a columnar mould and capable of immobilizing and loading various active biological enzymes such as protease, lactase, lipase and amylase, and the enzyme loading capacity of the porous base material is 133-216IU / g. The porous carbon material provides a large specific area, the high enzyme loading capacity can be achieved after crosslinking with the biological enzymes, high bioactivity of the biological enzymes after long-time enzymolysis treatment can be guaranteed, requirements on stability and continuity of enzymolysis treatment can be met, and production efficiency in biological enzyme treatment is improved substantially.
Owner:南京萌萌菌业有限公司
Preparation methods of immobilized nitrile group hydratase and (S)-N-ethylpyrrolidine-2-formamide
ActiveCN108660131AIncrease enzyme activityHigh enzyme loadingOn/in organic carrierFermentationFormamideHigh activity
The invention discloses a preparation method of immobilized nitrile group hydratase and a method for catalytic preparation of (S)-N-ethylpyrrolidine-2-formamide through the immobilized nitrile group hydratase. Firstly, a carbon nanotube is adopted to be subjected to carboxylation and acylating chlorination and then reacts with polysaccharide to prepare a polysaccharide-modified carbon nanotube composite, and then through absorption, the immobilized nitrile group hydratase having high activity and capable of being recycled is prepared. A substrate, namely 2-cyano group-N-ethylpyrrolidine is subjected to amidation by taking the immobilized nitrile group hydratase as a catalyst, and the (S)-N-ethylpyrrolidine-2-formamide product with the high yield and high optical activity is obtained. The process is concise, the application range is wide, environmental pollution is small, the atom utilization rate is high, and the preparation methods are each an environment-friendly process.
Owner:ZHEJIANG UNIV OF TECH
Phospholipid modified acrylon nano fiber composite film and its preparation and immobilized enzyme thereof
InactiveCN100371372CSufficient sourceSimple production processWet spinning methodsOn/in organic carrierFiberComposite film
The invention discloses a phosphatide modified acrylic nanometer fibre composite film and preparing and immobilized enzyme method, which comprises the following steps: taking dimethyl sulfoxide, dimethyl formamide or dimethylacetamide as solvent; preparing acrylon copolymer with phosphatide group through electrostatic spinning method as the carrier of immobilized enzyme; immobilizing the enzyme on the nanometer fiber composite film through physical adsorptive method. The invention applies bionic property of high specific surface area, high gap rate and phosphatide property, which produces high enzyme carrying, specific activity and catalyzing rate immobilizing enzyme phosphatide modified acrylic nanometer fibre composite film.
Owner:ZHEJIANG UNIV
A kind of preparation method and application of polym-phenylene isophthalamide-polyacrylonitrile composite nanofiber membrane
InactiveCN103726233BEasy to makeLarge specific surface areaFilament/thread formingNon-woven fabricsPolymer scienceAcrylonitrile
The invention relates to a method for preparing a nanofiber film by blending polym-phenylene isophthalamide and polyacrylonitrile and its application. Specifically: prepare a polym-phenylene isophthalamide polymer solution with a mass percentage of 5-8%; prepare a polyacrylonitrile solution with a mass percentage of 5-8%; mix the two solutions at a ratio of 3:1-1 : 5 mass ratio mixing, polym-phenylene isophthalamide-polyacrylonitrile composite nanofiber membrane is made by electrospinning method, as the immobilized carrier of enzyme; through sodium hydroxide / ethylenediamine surface reaction The immobilized carrier of the enzyme is modified by the method; the enzyme is immobilized on the nanofiber membrane with active amino groups and carboxyl groups on the surface. The invention has the advantages of simple carrier preparation and enzyme immobilization process, high enzyme loading capacity, high catalytic activity, high mechanical strength of the carrier, repeated use and the like.
Owner:TONGJI UNIV
Poly(methacrylic acid-itaconic acid) modified membrane, as well as preparation method and application thereof
ActiveCN109942879AEasy to makeSimple processOn/in organic carrierFermentationItaconic acidTransesterification
The invention provides a poly(methacrylic acid-itaconic acid) modified membrane, as well as a preparation method and application thereof. The preparation method comprises the following steps: mixing poly(methacrylic acid-itaconic acid)(IA-MAA), polyvinylpyrrolidone (PVP) and N,N-dimethyl formamide to obtain a casting solution; reacting the casting solution in a sealed condition at 55-65 DEG C for40-50 hours, defoaming, and preparing a flat membrane by adopting a tape casting method; soaking the flat membrane with the casting solution in a gel bath, and curing to form a membrane. The poly(methacrylic acid-itaconic acid) modified membrane prepared by a conversion method by using immobilized carrier as a phase, lipase is efficiently immobilized by modifying chitosan and activating glutaraldehyde. The method is simple in operation, the material has stable performance, the selectivity of transesterification can be greatly improved. The poly(methacrylic acid-itaconic acid) modified membranehas excellent industrial application prospect.
Owner:SOUTHEAST UNIV
Biocompatible metal-organic framework material glutamic acid-zinc and preparation and application thereof
ActiveCN106987580ALow costGenerate fastHydrolasesOrganic compound preparationPhosphateMetal-organic framework
The invention belongs to the technical field of novel materials and particularly relates to a biocompatible metal-organic framework material glutamic acid-zinc and preparation and an application thereof. The biocompatible metal-organic framework material glutamic acid-zinc is obtained through dropwise adding a disodium glutamate water solution into a methanol solution of Zn(NO3)2. The method has the characteristics of being high in glutamic acid-zinc crystal growth speed and mild in preparation condition, and the prepared product is good in crystal form, can be applied to a series of enzymes containing abundant amino, such as lipase, protease and epoxide hydrolase, and has the potential as a protein drug carrier. The product is added to an aspergillus niger lipase phosphate buffer solution modified by EDC or EDC.HC and NHS for shock immobilization to obtain the immobilized aspergillus niger lipase. The method is high in immobilization efficiency, high in enzyme recovery rate, simple in operation and mild in condition.
Owner:SOUTH CHINA UNIV OF TECH
Preparation method of laccase/multihole nanometer titanium dioxide modified electrode
InactiveCN102645469ALittle loss of activityHigh activityMaterial analysis by electric/magnetic meansCalcinationChemistry
The invention relates to a preparation method of a laccase / multihole nanometer titanium dioxide modified electrode. The preparation method comprises the steps of material preparation, preparation for combine use of hydrothermal method and calcination method of a multihole nanometer titanium dioxide carrier, adsorption and immobilization of laccase on the carrier and electrode modification. Immobilized laccase is uniformly scattered in Nafion dilute solution so as to modify a glassy carbon electrode which is used as a work electrode, so that a three-electrode system is established, and the three-electrode system is connected with an electrochemistry work station so as to detect the electrochemistry response of the three-electrode system on water pollutant hydroquinone through cyclic voltammetry. Compared with the prior art, the method disclosed by the invention has the advantages that the cost is low, the preparation is simple, the enzyme activity of the immobilized laccase is high, the sensibility is high, and the like.
Owner:TONGJI UNIV
Method for immobilizing lipase by block polymer micelle
ActiveCN109706140ALarge specific surface areaHigh porosityOn/in organic carrierFermentationEnzymatic synthesisFreeze-drying
The invention provides a method for immobilizing lipase by block polymer micelle. The method comprises the following steps: dithiodipropionic acid is activated by an activator to activate a carboxyl group and reacted with hyaluronic acid to prepare an intermediate, the intermediate is activated by the activator to react with polylactic acid-glycolic acid to obtain a block polymer, and the block polymer is dissolved in deionized water to prepare a block polymer micelle solution; the lipase powder is dissolved in a phosphate buffered saline solution of pH being 8.0, and the material is stirred and centrifuged, a supernatant is transferred to a volumetric flask, and a volume is metered with a buffer solution of pH being 8.0 to obtain enzymatically synthesized cyhalofoprin lipase; the polymermicelle solution is mixed with the enzymatically synthesized cyhalofoprin lipase, a mixture is filtered, deionized water is added for dissolving, and washed with the buffer solution of pH being 8.0 toremove excess lipase, a volume is metered to the 1L of a phosphate buffer solution of the pH being 8.0, and lipase immobilized by block polymer micelle can be obtained after freeze drying. The process of the invention is simple.
Owner:江苏丰山生化科技有限公司
Preparation method of genipin
ActiveCN106701858AImprove efficiencyHigh enzyme loadingFermentationOn/in inorganic carrierAlgluceraseDispersity
The invention discloses a preparation method of genipin. The preparation method comprises the following steps: carrying out glutaraldehyde crosslinking on amination-modified nano SiO2 particles and beta-glucosaccharase to obtain a nano immobilized enzyme, and carrying out high-efficiency hydrolysis on the substrate jasminoidin by using the nano immobilized enzyme to obtain the genipin. The nano immobilized enzyme prepared in the method has favorable dispersity and is recyclable in the reaction system; and the method has the advantages of high genipin yield and purity, and thus, can effectively prepare genipin.
Owner:NANJING UNIV OF SCI & TECH
Method for immobilizing enzyme by using polydopamine-coated plastic filler
PendingCN113584011ALow costImprove surface roughnessImmobilised enzymesHydrolasesPolymer scienceOrganosolv
The invention relates to a method for immobilizing enzyme by using polydopamine-coated plastic filler. The method comprises the following steps: putting a plastic filler into a dopamine solution with a proper concentration, carrying out a constant-temperature oscillation reaction, carrying out surface modification to enable the plastic filler to have better adhesion, wettability and surface roughness, and loading sol containing an immobilized enzyme solution on the modified filler. The method is low in cost, good in effect and easy and convenient to operate, and the obtained plastic enzyme-loaded filler has higher acid-base stability, organic solvent stability, storage stability and reusability.
Owner:HEBEI UNIV OF TECH
Dendrobium officinale Kimura et Migo extracting solution fermented wine and preparation method thereof
PendingCN112592793AIncrease profitIncrease contentAlcoholic beverage preparationBiotechnologyNutritive values
The invention provides a preparation method of dendrobium officinale Kimura et Migo extracting solution fermented wine, and belongs to the technical field of beverage food and processing thereof. Thepreparation method comprises the steps of raw material treatment, water extraction, enzymolysis and fermentation, and specifically comprises the following steps of: cleaning fresh dendrobium officinale Kimura et Migo, drying, crushing and sieving; performing sealed heating extraction on the treated dendrobium officinale Kimura et Migo by taking water as a solvent to obtain a water extracting solution; adding compound protease into the water extracting solution, adjusting the pH and temperature of the water extracting solution to adapt to the compound protease, performing sealed heat-preservation enzymolysis, performing enzyme deactivation treatment after the enzymolysis is finished, and performing centrifugal separation on supernatant to obtain enzymatic hydrolysate; and adjusting the sugar degree and pH, performing high-temperature sterilization, inoculating saccharomyces cerevisiae, and performing constant-temperature fermentation to obtain the dendrobium officinale Kimura et Migo extracting solution fermented wine. According to the preparation method, active components in the dendrobium officinale Kimura et Migo are fully dissolved out by a water extraction method, and then enzymolysis and fermentation are performed, so that the utilization rate of the dendrobium officinale Kimura et Migo and the nutritional value of a product are increased.
Owner:SOUTHWEST FORESTRY UNIVERSITY
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