The invention provides a preparation method of dendrobium officinale Kimura et Migo extracting solution fermented wine, and belongs to the technical field of beverage food and processing thereof. Thepreparation method comprises the steps of raw material treatment, water extraction, enzymolysis and fermentation, and specifically comprises the following steps of: cleaning fresh dendrobium officinale Kimura et Migo, drying, crushing and sieving; performing sealed heating extraction on the treated dendrobium officinale Kimura et Migo by taking water as a solvent to obtain a water extracting solution; adding compound protease into the water extracting solution, adjusting the pH and temperature of the water extracting solution to adapt to the compound protease, performing sealed heat-preservation enzymolysis, performing enzyme deactivation treatment after the enzymolysis is finished, and performing centrifugal separation on supernatant to obtain enzymatic hydrolysate; and adjusting the sugar degree and pH, performing high-temperature sterilization, inoculating saccharomyces cerevisiae, and performing constant-temperature fermentation to obtain the dendrobium officinale Kimura et Migo extracting solution fermented wine. According to the preparation method, active components in the dendrobium officinale Kimura et Migo are fully dissolved out by a water extraction method, and then enzymolysis and fermentation are performed, so that the utilization rate of the dendrobium officinale Kimura et Migo and the nutritional value of a product are increased.