55results about How to "High enzyme loading" patented technology

The invention discloses a phosphatide modified acrylic nanometer fibre composite film and preparing and immobilized enzyme method, which comprises the following steps: taking dimethyl sulfoxide, dimethyl formamide or dimethylacetamide as solvent; preparing acrylon copolymer with phosphatide group through electrostatic spinning method as the carrier of immobilized enzyme; immobilizing the enzyme on the nanometer fiber composite film through physical adsorptive method. The invention applies bionic property of high specific surface area, high gap rate and phosphatide property, which produces high enzyme carrying, specific activity and catalyzing rate immobilizing enzyme phosphatide modified acrylic nanometer fibre composite film.

The invention relates to a method for preparing a nanofiber film by blending polym-phenylene isophthalamide and polyacrylonitrile and its application. Specifically: prepare a polym-phenylene isophthalamide polymer solution with a mass percentage of 5-8%; prepare a polyacrylonitrile solution with a mass percentage of 5-8%; mix the two solutions at a ratio of 3:1-1 : 5 mass ratio mixing, polym-phenylene isophthalamide-polyacrylonitrile composite nanofiber membrane is made by electrospinning method, as the immobilized carrier of enzyme; through sodium hydroxide / ethylenediamine surface reaction The immobilized carrier of the enzyme is modified by the method; the enzyme is immobilized on the nanofiber membrane with active amino groups and carboxyl groups on the surface. The invention has the advantages of simple carrier preparation and enzyme immobilization process, high enzyme loading capacity, high catalytic activity, high mechanical strength of the carrier, repeated use and the like.

The invention belongs to the technical field of novel materials and particularly relates to a biocompatible metal-organic framework material glutamic acid-zinc and preparation and an application thereof. The biocompatible metal-organic framework material glutamic acid-zinc is obtained through dropwise adding a disodium glutamate water solution into a methanol solution of Zn(NO3)2. The method has the characteristics of being high in glutamic acid-zinc crystal growth speed and mild in preparation condition, and the prepared product is good in crystal form, can be applied to a series of enzymes containing abundant amino, such as lipase, protease and epoxide hydrolase, and has the potential as a protein drug carrier. The product is added to an aspergillus niger lipase phosphate buffer solution modified by EDC or EDC.HC and NHS for shock immobilization to obtain the immobilized aspergillus niger lipase. The method is high in immobilization efficiency, high in enzyme recovery rate, simple in operation and mild in condition.

The invention relates to an immobilized enzyme membrane reactor and the preparation thereof and a method for producing bio-diesel. The method comprises filling superfine fiber complex film in a die whose inside and outside are double cylindrical shape to form a film reactor, injecting activating agent solution and lipase solution in the film reactor in turn through adopting a cross-flow filtration mode, leading lipase to be chemically fixed on superfine fiber membrane surface, getting an enzyme membrane reactor which is fixed with lipase, respectively injecting mixed solution of animal and vegetable oil and carbinol in the inner side and the outer side of the superfine fiber complex film, doing catalytic alcoholysis reaction on fiber membrane surface, and producing bio-diesel. The enzyme gathering catalysis of the method of the invention is integral with a membrane separation process, and the method of the invention simplifies manufacturing technique, and meanwhile, the method of the invention overcomes inhabitation of lower alcohol to enzymatic activity in the process of catalyzing, the immobilized enzyme membrane reactor can be repeatedly used, which can achieve continuous production, improves production efficiency, and has excellent industrial application prospect.