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Preparation method of block polymer immobilized enzyme

A technology of block polymer, immobilized enzyme, applied in the direction of immobilization on/in organic carrier, biochemical fiber treatment, non-woven fabric, etc. The process of immobilizing enzymes is cumbersome and other problems, so as to achieve a good microenvironment, improve biological activity and stability, and improve the utilization rate of enzymes.

Inactive Publication Date: 2013-08-21
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of the low enzyme loading and catalytic efficiency of some immobilized enzyme carriers, the complicated immobilized enzyme process and difficult recovery of carrier materials, the purpose of the present invention is to provide a A preparation method of block polymer immobilized enzyme with high specific surface area, high porosity, certain mechanical strength and simple preparation process

Method used

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Examples

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Effect test

Embodiment 1

[0022] 1. Dissolve 1 gram of polymethyl methacrylate-ethyl acrylate block polymer in N,N-dimethylformamide and tetrahydrofuran solution with a mass ratio of 1:1 to form a 5% solution by weight , after being fully dissolved, inject it into the electrospinning device, carry out electrospinning under the condition that the voltage is 18 kV, the flow rate of the spinneret solution is 0.5 ml / hour, and the receiving distance is 15 cm, and the fiber diameter is 200 nm. For the methyl methacrylate-ethyl acrylate nanofiber membrane, the nanofiber membrane was dried in a vacuum oven at 30° C. for 5 hours.

[0023] 2. Immerse 1 gram of polymethyl methacrylate-ethyl acrylate nanofiber membrane in 4% polyethylenediamine aqueous solution, seal it and shake it at a constant temperature of 70°C for 1 hour at a speed of 50 rpm ; Take out the nanofiber membrane and rinse it fully with a phosphate buffer solution with a pH of 7.0 to remove the residual polyethylenediamine on the membrane; immers...

Embodiment 2

[0025] 1. Dissolve 1 gram of polymethyl methacrylate-ethyl acrylate block polymer in N,N-dimethylformamide and tetrahydrofuran solution with a mass ratio of 1:4 to form a 10% solution by weight , after being fully dissolved, inject it into the electrospinning device, carry out electrospinning at a voltage of 25 kV, a flow rate of the spinneret solution of 1.5 ml / hour, and a receiving distance of 20 cm to obtain a fiber diameter of 300 nm. For the methyl methacrylate-ethyl acrylate nanofiber membrane, the nanofiber membrane was dried in a vacuum oven at 50° C. for 10 hours.

[0026] 2. Immerse 1 gram of polymethyl methacrylate-ethyl acrylate nanofiber membrane in 8% polyethylenediamine aqueous solution, seal it and shake it at a constant temperature of 70°C for 2 hours at a speed of 80 rpm ; Take out the nanofibrous membrane and rinse it fully with a phosphate buffer solution with a pH of 7.0 to remove the residual polyethylenediamine on the membrane; immerse the aminated nanof...

Embodiment 3

[0029] 1. Dissolve 1 gram of polymethyl methacrylate-ethyl acrylate block polymer in N,N-dimethylformamide and tetrahydrofuran solution with a mass ratio of 1:2 to form a solution with a weight percentage of 8%. , after being fully dissolved, inject it into the electrospinning device, carry out electrospinning under the condition that the voltage is 15 kV, the flow rate of the spinneret solution is 0.8 ml / hour, and the receiving distance is 15 cm, and the fiber diameter is 300 nm. For the methyl methacrylate-ethyl acrylate nanofiber membrane, the nanofiber membrane was dried in a vacuum oven at 30° C. for 5 hours.

[0030]2. Immerse 1 gram of polymethyl methacrylate-ethyl acrylate nanofiber membrane in 6% polyethylenediamine aqueous solution, seal it and shake it at a constant temperature of 70°C for 1.5 hours at a speed of 60 rpm ; Take out the nanofibrous membrane and rinse it fully with a phosphate buffer solution with a pH of 7.0 to remove the residual polyethylenediamine ...

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Abstract

The invention relates to a preparation method of a block polymer immobilized enzyme. The method comprises the following steps of dissolving polymethyl methacrylate-ethyl acrylate block polymer into N,N-dimethylformamide and tetrahydrofuran which are in a certain mass ratio; preparing polymethyl methacrylate-ethyl acrylate nano fiber film through an electrostatic spinning method; with the polymethyl methacrylate- ethyl acrylate nano fiber film as an immobilized carrier of the enzyme, and fixing the enzyme onto the nano fiber film through a method for ammonificating polyethylenimine and activating glutaraldehyde. By utilizing the high specific surface area and high void ratio of the nano fiber film, the nano fiber film immobilized enzyme with high enzyme carrying capacity and high catalyzing efficiency can be obtained. The immobilized enzyme has the advantages that the immobilized enzyme is easily recovered from a reaction system and can be reutilized for multiple times, the utilization rate of the enzyme can be increased, the production cost can be reduced, and the preparation process is simple.

Description

technical field [0001] The invention relates to a preparation method of block polymer immobilized enzyme. Background technique [0002] The catalytic activity of free enzymes is easily affected by factors such as pH, temperature, organic solvents, and heavy metal ions. The enzyme immobilization technology can effectively improve the stability and catalytic activity of the enzyme under extreme conditions, and it is easy to recycle and reuse. There are many materials and methods for immobilizing enzymes, among which nanomaterials are favored by researchers because of their high enzyme loading capacity, especially some carrier materials with porous structure and large specific surface area are beneficial to improve the immobilization of enzymes. Enzyme load, and facilitate the diffusion of the substrate to the enzyme active center and the product to the outside of the reaction area to improve the biological activity and catalytic efficiency of the immobilized enzyme. [0003]...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/08D04H1/4326D04H1/728D06M15/61D06M13/123D06M16/00D06M101/26
Inventor 徐冉李风亭迟成龙
Owner TONGJI UNIV
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