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Preparation method of laccase/multihole nanometer titanium dioxide modified electrode

A nano-titanium dioxide and modified electrode technology, applied in the field of biosensors, can solve the problems of inability to timely online monitoring, expensive analytical instruments, strong toxicity, etc., and achieve the effects of large application potential, strong electronic conductivity, and strong activity.

Inactive Publication Date: 2012-08-22
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Hydroquinone is widely used in the industrial field, and is one of the highly toxic and difficult to degrade phenolic pollutants. The existing detection methods for hydroquinone mainly use chromatography, which has the disadvantages of expensive analytical instruments and difficult operation. Complicated, defects that cannot be monitored online in time

Method used

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  • Preparation method of laccase/multihole nanometer titanium dioxide modified electrode
  • Preparation method of laccase/multihole nanometer titanium dioxide modified electrode
  • Preparation method of laccase/multihole nanometer titanium dioxide modified electrode

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Embodiment 1

[0030] A kind of preparation method of laccase / porous nano-titanium dioxide modified electrode, the method comprises the following steps:

[0031] Preparation of Porous Nano-TiO Microsphere Support

[0032] (1) Prepared by hydrothermal synthesis method, 20mg Ti(CP) 2 Cl 2 Disperse in 10ml of deionized water in the reaction kettle, and add 2-3 drops of ethylenediamine dropwise. Hydrothermal reaction at 120°C for 6 hours, cooled to room temperature, and centrifuged and washed three times with water and absolute ethanol;

[0033] (2) Calcining the sample obtained in step (1) at 400° C. for 2 hours in a tube furnace, controlling the temperature rise and fall at a rate of 3 to 4° C. / s to obtain a porous nano-titanium dioxide microsphere carrier. The SEM image and XRD image of the prepared porous nano-titanium dioxide microsphere carrier are as follows: figure 1 , figure 2 shown.

Embodiment 2

[0035] A kind of preparation method of laccase / porous nano-titanium dioxide modified electrode, the method comprises the following steps:

[0036] Preparation of Laccase / Porous Nano-TiO2 Immobilized Enzyme

[0037] (1) Weigh 20mg of the carrier and mix it with 5ml of dilute laccase solution, sonicate at room temperature (about 6°C) for 10 minutes, and then put it into a shaking water tank at room temperature (about 4°C) for adsorption and immobilization for 12 hours.

[0038] (2) The immobilized enzyme prepared in step (1) was washed with deionized water for 6 times, dried at room temperature, sealed and stored at 4° C. together with color-changing silica gel. Comparison of Fourier infrared images of pure laccase, porous nano-titanium dioxide microsphere carrier and laccase / porous nano-titanium dioxide immobilized enzyme image 3 Shown, wherein a is porous nano-titanium dioxide microsphere carrier, b is laccase / porous nano-titanium dioxide immobilized enzyme, and c is pure la...

Embodiment 3

[0040] A kind of preparation method of laccase / porous nano-titanium dioxide modified electrode, the method comprises the following steps:

[0041] Preparation of Laccase / Porous Nano-TiO Modified Electrode

[0042] (1) Weigh 1 mg of laccase / porous nano-titanium dioxide immobilized enzyme and dissolve it in 5 ml of 0.05 wt% tetrafluoroethylene and perfluoro-2-(sulfonic acid ethoxy) propyl vinyl ether copolymer (Nafion) solution, at room temperature (about 6°C) sonicate for 3 minutes to make it evenly mixed;

[0043] (2) Put the glassy carbon electrode on the polishing cloth with the particle size of 0.3μm and 0.05μm 2 o 3 The powder was polished and ultrasonically cleaned with concentrated nitric acid, distilled water and absolute ethanol for 5 min, and then dried under an infrared lamp, and 10 μl of evenly dispersed laccase / porous nano-TiO 2 / Nafion mixed liquid is dropped on a clean glassy carbon electrode and dried naturally at room temperature to obtain laccase / porous nan...

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Abstract

The invention relates to a preparation method of a laccase / multihole nanometer titanium dioxide modified electrode. The preparation method comprises the steps of material preparation, preparation for combine use of hydrothermal method and calcination method of a multihole nanometer titanium dioxide carrier, adsorption and immobilization of laccase on the carrier and electrode modification. Immobilized laccase is uniformly scattered in Nafion dilute solution so as to modify a glassy carbon electrode which is used as a work electrode, so that a three-electrode system is established, and the three-electrode system is connected with an electrochemistry work station so as to detect the electrochemistry response of the three-electrode system on water pollutant hydroquinone through cyclic voltammetry. Compared with the prior art, the method disclosed by the invention has the advantages that the cost is low, the preparation is simple, the enzyme activity of the immobilized laccase is high, the sensibility is high, and the like.

Description

technical field [0001] The invention relates to the field of biosensors, in particular to a preparation method for modifying electrodes with laccase / porous nano titanium dioxide. Background technique [0002] Phenolic substances exist widely in the environment. As a toxic substance, they have caused great harm to human health. Therefore, the detection of phenolic substances has become an urgent problem to be solved, and it is also of great significance to environmental monitoring. . Hydroquinone is widely used in the industrial field, and is one of the highly toxic and difficult to degrade phenolic pollutants. The existing detection methods for hydroquinone mainly use chromatography, which has the disadvantages of expensive analytical instruments and difficult operation. Complicated, defects that cannot be monitored online in time. Biosensor refers to the use of immobilized biological components (such as: enzymes, antigens, antibodies, hormones, etc.) or the organism itsel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327
Inventor 吴庆生毛琦谦朱铁建
Owner TONGJI UNIV
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