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Application of poly gamma glutamic acid as bacillus thuringiensis agent protector

A technology of Bacillus thuringiensis and Bacillus aureus, applied in bacteria, microorganism-based methods, microorganisms, etc., can solve the problems of incomplete elucidation of the protection mechanism, weakened insecticidal activity, and short residual effect period, so as to improve the resistance to ultraviolet rays in sunlight. Effect of irradiating ability, reducing activity loss, prolonging residual period

Inactive Publication Date: 2007-04-18
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are also some problems in its production and application process, which lead to the weakening or even failure of its insecticidal activity, short residual effect period and other shortcomings: (1) The spray drying process causes its activity to decrease
From the chemical structure point of view, both alginate and poly-α-glutamic acid contain many free carboxyl groups with high reactivity in the long chain of the polymer, but the protection mechanism of the two has not been fully elucidated so far.

Method used

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  • Application of poly gamma glutamic acid as bacillus thuringiensis agent protector
  • Application of poly gamma glutamic acid as bacillus thuringiensis agent protector
  • Application of poly gamma glutamic acid as bacillus thuringiensis agent protector

Examples

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Effect test

Embodiment 1

[0035] Embodiment 1: The application of γ-PGA as the protective agent in the spray-drying process of Bt fermented liquid

[0036] 0g, 0.2g, 0.4g, 0.6g, 0.8g, 1.0g, 1.2g, 1.4g, 1.8g, 2.0g of γ-PGA with a molecular weight of 100,000-10,000,000 Daltons were dissolved in 200ml Bt fermentation broth, The insecticidal crystal protein content of the fermentation broth was 5.43mg / ml, and the number of spores was 6.77×10 9 cfu / ml, the lethal rate (when diluted 4000 times) is 91.27%. After stirring evenly, carry out spray drying respectively. The spray drying process conditions are: the air inlet temperature is 180±5°C, the air outlet temperature is 70±2°C, and the nozzle pressure is 0.15Mpa, so that the water content of the preparation is controlled below 4%. Measure insecticidal crystal protein, spores and insecticidal activity of Bt preparation after spray drying under different conditions, and calculate insecticidal crystal protein residual rate and spore residual rate with Bt prep...

Embodiment 2

[0044] Example 2: The application of γ-PGA as a protective agent under different air outlet temperature conditions in the spray drying process of Bt fermentation broth

[0045] Take 3.6g of γ-PGA with a molecular weight of 100,000-10,000,000 Daltons and dissolve it in 1200ml of Bt fermentation broth. The content of insecticidal crystal protein in the fermentation broth is 5.43mg / ml, and the number of spores is 6.77×10 9 cfu / ml, the lethal rate (when diluted 4000 times) is 91.27%. After stirring evenly, divide it into six parts on average, and carry out spray drying respectively. When investigating the influence of γ-PGA on the spore and crystal content of spray-dried Bt raw powder at different air outlet temperatures, the air inlet temperature was controlled at 180±5°C, the air outlet temperature was 50-100±5°C, and the nozzle pressure was 0.15Mpa, so that the preparation The water content is controlled below 4%. The residual biological activity of Bt preparations was measur...

Embodiment 3

[0050] Example 3: The application of γ-PGA as a protective agent under different air inlet temperature conditions in the spray drying process of Bt fermentation broth

[0051] Take 3.6g of gamma-PGA whose molecular weight is 100,000-10,000,000 Daltons and dissolve it in 1200ml of Bt fermented liquid. 9 cfu / ml, the lethal rate (when diluted 4000 times) is 91.27%. After stirring evenly, divide it into six parts and spray dry them respectively. When investigating the influence of γ-PGA on the spore and crystal content of spray-dried Bt raw powder at different air inlet temperatures, the air outlet temperature was controlled at 70±2°C, the air inlet temperature was 150-250°C, and the nozzle pressure was 0.15Mpa, so that the water content of the preparation Control it below 4%. The residual biological activity of Bt preparations was measured under different conditions.

[0052] The experimental results are shown in Table 2 and Figure 3:

[0053] Amount of PGA added

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Abstract

This invention belongs to preparative technique field of Biological Pesticide, Specifically relating to a gamma-PGA which is used as protective additive for Bacillus thuringiensis praeparatum and the its application that it is used as protective agent in thuringiensis praeparatum preparation . using gamma-PGA as Bt praeparatum, can reduce activity loss of Bt praeparatum in spray drying process, compared with control group, the anthelminthic activity, crystalline and gemma content of invented praeparatum raise about 18.55%,11.24% and 20.38% respectively. Besides, the application of this invention elevates anti- ultraviolet irradiation capability of Bt praeparatum in practically using process, after Sunlight shines 7 days, its anthelminthic activity is 4.30 times of control group. The application of this invention elevates sticky and attaching capability of Bt praeparatum at plant strain. It bears rain water underscouring, its anthelminthic activity can achieve more than 2.23 times of control group, accordingly extend residual period.

Description

technical field [0001] The invention belongs to the technical field of preparation of microbial pesticides, and in particular relates to the use of poly-γ-glutamic acid as a protective agent for Bacillus thuringiensis preparations and its application as a protective agent in the preparation of Bacillus thuringiensis preparations. Background technique [0002] Resource and environmental issues have become major issues facing the world's human beings. According to the investigation of the Food and Agriculture Organization of the United Nations, the economic loss of grains caused by pests and diseases in the world reaches 120 billion US dollars every year. In order to deal with pests and diseases, chemical pesticides have been used for a long time, in large quantities and repeatedly, causing pollution to soil, water and the atmosphere; the increase of pesticide residues in agricultural by-products has also directly endangered human health and sur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/07
Inventor 陈守文喻子牛吴广涛蔡皓冀志霞孙明柯云
Owner HUAZHONG AGRI UNIV
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