34results about How to "Increased spore content" patented technology

A process for preparing the biologic agricultural chemical from trichoderma includes such steps as reproductive culture of trichoderma, fermenting in liquid, inoculating it in the culture medium prepared from cane dregs, wheat bran and rice husk for solid fermenting, culturing at 25-28 deg.C under 60-80% of relative humidity for 4-5 days, culturing at low temp (lower than 12 deg.C) for 2-3 days, drying at 40-50 deg.C for 12-24 hr, adding promoter prepared from diatomite, wheat bran and sulfur, mixing, pulverizing and sieving.

The invention discloses gene recombination bacillus amyloliquefaciens and a microbial inoculum preparation method. A bacillus amyloliquefaciens FZB42 strain is taken as a host bacterium to construct the gene recombination bacillus amyloliquefaciens XLV09-1 with transparent vitreoscilla hemoglobin which is regulated and controlled by a P43 strong promoter by contrasting a gene recombination vector. The produced spore number of microbial inoculum prepared by fermenting the bacillus amyloliquefaciens can reach more than 100 hundred millions per milliliter, the overall yield of antibacterial lipopeptid reaches more than 1 gram per liter and prevention effect on various fungal plant diseases is improved by over 30 percent. The microbial inoculum is a green microecological preparation, of no toxic residue, safe to human and livestock, and favorable for protecting the ecological environment.

The invention discloses a bacillus subtilis (Bacillius subtilis) strain, the strain preservation number of which is CGMCC No. 1982 in the General Microorganism Center (CGMCC) of China Microorganism Culture Collection Management Committee. The dry powder bacterial agent made from the strain can be used to prevent and control crops, vegetables and other diseases caused by wheat scab, rice blast fungus, corn leaf spot fungus, cucumber anthracnose fungus, garlic sclerotinia, cucumber anthracnose fungus, etc.; It is used in the fresh-keeping field of peaches, apples, grapes, pears and other fruits. The rice and corn leaves treated with the bacterial suspension will not have corresponding disease spots; the cucumber seeds or seedling leaves treated with powder seed dressing, powder root burial or bacterial suspension spraying can not only prevent cucumber anthracnose Occurs, and shows a good growth-promoting effect, which is conducive to the production of green and pollution-free vegetables. The rotten fruit rate of fresh peaches treated with bacterial suspension was significantly reduced and the storage period was prolonged. The bacterial agent has a simple preparation process and is suitable for industrial production.

The invention relates to the technical field of preparation of microbes and specifically relates to a method for efficiently improving the germination rate of Bacillus subtilis. According to the method provided by the invention, glycerine is added in a Bacillus subtilis fermentation broth as a fermentation strain, an inorganic salt solution capable of improving the spore germination rate is added when the fermentation strain is cultured for 15-18 hours, and the inorganic salt solution contains the following components in percentage by weight: 0.12-0.125% of MgSO4.7H2O, 0.01-0.015% of MnSO4.H2O, 0.05-0.1% of CaCl2, 0.005-0.008% of FeSO4.7H2O, 0.015-0.02% of KI and 0.035-0.04% of KH2PO4. The fermentation of the strain obtained by the method can achieve 5.8*10<11> CFU / ml, the number of spores is 5.49*10<11> CUF / ml, and the spore germination rate is 94.7%.

The invention provides a preparation method of Streptomyces jingyangensis wettable powder. The method comprises the following steps: fermenting a carrier kaolin, Rhodopseudomonas palustris, and nutrients corn starch and bean pulp powder to obtain high-concentration Streptomyces jingyangensis powder; and mixing 90-95 parts of Streptomyces jingyangensis fermented powder, 1-4 parts of wetting agent sodium dodecyl benzene sulfonate, 0.2-1 part of dispersing agent tea saponin, 0.5-2 parts of ultraviolet protective agent ascorbic acid, 0.2-1 part of beta-dextrin, 1-4 parts of drying protective agent maltose and 0.2-1 part of mannitol to obtain the stable Streptomyces jingyangensis wettable powder with the bacterium content of more than 10 billion CFU / g.

The invention discloses a Paenibacillus polymyxa GY40 strain, and its preservation number is: CGMCC NO.14481. The invention also discloses a spore-forming fermentation culture method of the Paenibacillus polymyxa GY40 strain and a method for preparing bacteria powder and wettable powder by using the Paenibacillus polymyxa GY40 strain. The bacterial strain of the present invention has the advantages of strong salt tolerance, low growth temperature, and strong antibacterial effect that terrestrial microorganisms do not have; its spore-forming fermentation method has a simple formula, and the raw materials are mainly agricultural product leftovers, with low price, wide sources, and rapid growth of the bacterial strain. Fast, low fermentation temperature, short fermentation time, easy control of fermentation conditions, high yield of fermentation spores, reduced cost of raw materials, reduced energy consumption of fermentation, improved fermentation production efficiency, the wettable powder prepared by this strain has good control Wheat head blight and cucumber wilt can be used for wheat and cucumber planting.

The invention discloses a fungicide containing bacillusamyloliquefaciens and application thereof, belonging to the field of microorganisms. The water content of the fungicide is not higher than 35%. The fungicide is prepared in a way of absorbing a bacillus fermentation liquid by using a carrier. The fungicide disclosed by the invention has the advantages of organic fertilizers and microbial fertilizers and has the functions of improving the utilization ratio of the fertilizer, increasing soil organic matters, promoting the breeding of rhizosphere microorganisms of crops and improving the physicochemical property and biological activity of soil. The fungicide provides comprehensive and balanced nutrition for growth of crops and can prevent and reduce soil-borne diseases, so that the yield and quality of crops are remarkably enhanced.

The invention provides a method for culturing brevibacillus brevis and relates to the field of bio-pesticides. The method culturing the brevibacillus brevis by a fermentation medium containing the following components: 5-7 g / L of molasses, 7-9 g / L of corn starch, 14-16 g / L of soybean cake meal, 9-11 g / L of corn steep liquor, 4-6 g / L of yeast powder, 4-5 g / L of ammonium chloride, 4.5-5 g / L of calcium carbonate, 1.2-1.7 g / L of dipotassium phosphate, 1.8-2.2 g / L of zinc sulfate, and the pH is 7.0-7.5. The method for culturing brevibacillus brevis can obtain more viable bacteria in a shorter time,and the spore content is higher.

This application relates to the field of microbial fermentation, and specifically discloses a fermentation process of bacterial enzyme feed, including the following steps: S1, preparing koji, dissolving the koji to obtain feed seed liquid; the koji is composed of koji materials and mixed bacteria powder; the mixed bacteria powder is composed of yeast powder and Bacillus subtilis powder; S2, the feed seed liquid prepared in S1 is stirred and fermented at 28-38°C for 68-78h, and then fermented at 47-58 Stir and dry for 32-40 hours under the condition of ℃ to prepare the finished bacterial enzyme feed; the bacterial enzyme feed has good storage stability in a higher temperature environment, even if the animal cannot eat it all at once, the remaining bacterial enzyme feed is exposed to relatively high temperature. Under high ambient temperature, the nutrients in the remaining bacterial enzyme feed still have good biological activity, and the animal can still effectively stimulate the animal's feed intake and improve the digestion and absorption rate after eating.

The invention provides a culture medium and application of Bacillus amyloliquefaciens, and relates to the field of microorganisms. The fermentation medium contains 5-10 g / L of glucose, 10-30 g / L of molasses, 5-10 g / L of silkworm chrysalis powder, 5-20 g / L of corn steep liquor, 10-20 g / L of sodium chloride, and 1-10 g of calcium carbonate / L, magnesium sulfate 0.1~1g / L, manganese sulfate 0.01~0.1g / L, ferrous chloride 0.02~0.08g / L, pH7.0~7.5. Use this medium to cultivate Bacillus amyloliquefaciens K-8, and control the culture conditions as follows: 0h-8h: gas-liquid ratio (0.4-0.6): 1, temperature 25°C-28°C; 8h-24h : The gas-liquid ratio is (0.9~1.1):1, the temperature is 28℃~30℃; after 24 hours: the gas-liquid ratio is (0.7~0.9):1, the temperature is 30℃~32℃. Adopting the culture medium and the culture method of the present invention is beneficial to the mass reproduction of bacillus amyloliquefaciens K-8 in a relatively short period of time, so that a large number of spores can be formed in a relatively short period of time.