34results about How to "Increased spore content" patented technology

A process for preparing the biologic agricultural chemical from trichoderma includes such steps as reproductive culture of trichoderma, fermenting in liquid, inoculating it in the culture medium prepared from cane dregs, wheat bran and rice husk for solid fermenting, culturing at 25-28 deg.C under 60-80% of relative humidity for 4-5 days, culturing at low temp (lower than 12 deg.C) for 2-3 days, drying at 40-50 deg.C for 12-24 hr, adding promoter prepared from diatomite, wheat bran and sulfur, mixing, pulverizing and sieving.

The invention discloses gene recombination bacillus amyloliquefaciens and a microbial inoculum preparation method. A bacillus amyloliquefaciens FZB42 strain is taken as a host bacterium to construct the gene recombination bacillus amyloliquefaciens XLV09-1 with transparent vitreoscilla hemoglobin which is regulated and controlled by a P43 strong promoter by contrasting a gene recombination vector. The produced spore number of microbial inoculum prepared by fermenting the bacillus amyloliquefaciens can reach more than 100 hundred millions per milliliter, the overall yield of antibacterial lipopeptid reaches more than 1 gram per liter and prevention effect on various fungal plant diseases is improved by over 30 percent. The microbial inoculum is a green microecological preparation, of no toxic residue, safe to human and livestock, and favorable for protecting the ecological environment.

The invention discloses a bacillus subtilis (Bacillius subtilis) strain, the strain preservation number of which is CGMCC No. 1982 in the General Microorganism Center (CGMCC) of China Microorganism Culture Collection Management Committee. The dry powder bacterial agent made from the strain can be used to prevent and control crops, vegetables and other diseases caused by wheat scab, rice blast fungus, corn leaf spot fungus, cucumber anthracnose fungus, garlic sclerotinia, cucumber anthracnose fungus, etc.; It is used in the fresh-keeping field of peaches, apples, grapes, pears and other fruits. The rice and corn leaves treated with the bacterial suspension will not have corresponding disease spots; the cucumber seeds or seedling leaves treated with powder seed dressing, powder root burial or bacterial suspension spraying can not only prevent cucumber anthracnose Occurs, and shows a good growth-promoting effect, which is conducive to the production of green and pollution-free vegetables. The rotten fruit rate of fresh peaches treated with bacterial suspension was significantly reduced and the storage period was prolonged. The bacterial agent has a simple preparation process and is suitable for industrial production.