Fermentation production method for hypocrea virens and solid medium of hypocrea virens
A solid medium and solid culture technology, applied in the field of microbial fermentation, can solve the problems of high cost, unsatisfactory, long fermentation time, etc.
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Embodiment 1
[0067] The H. chlorogenic strain provided by Jiangxi Tianren Ecological Co., Ltd. was inoculated with 1% on the PDA solid slant medium, and cultured at 28°C for 3 days to complete the activation of the strain. The activated H. chlorogenic strains were inoculated into a 250L fermenter equipped with a primary seed medium for liquid cultivation for 40 hours according to the inoculation amount of 1%. The culture temperature was 27°C and the initial pH value was 6.0. The primary seed medium The formula includes 1% of glucose, 1% of white sugar, 1% of soybean powder, 0.2% of ammonium sulfate, 0.2% of dipotassium hydrogen phosphate, 0.08% of potassium chloride, 0.02% of magnesium sulfate, 0.0002% of iron sulfate, and the balance is water. 24h after cultivation, according to 15m 3 / h ventilation for aeration culture, after 24 hours of liquid culture, according to 24m 3 The aeration rate per hour was used for aeration culture, the temperature of the liquid culture process was controll...
Embodiment 2
[0070] The H. chlorogenic strain provided by Jiangxi Tianren Ecological Co., Ltd. was inoculated with 0.8% on the PDA solid slant medium, and activated at 30°C for 4 days to complete the activation of the strain. The activated H. chlorogenic strains were inoculated into a 250L fermenter equipped with a primary seed medium for liquid cultivation for 50 hours according to an inoculation amount of 1.5%. The culture temperature was 28°C and the initial pH value was 6.5. The primary seed medium The formula includes 0.5% of glucose, 2.0% of white sugar, 0.3% of soybean powder, 0.1% of ammonium sulfate, 0.2% of dipotassium hydrogen phosphate, 0.05% of potassium chloride, 0.04% of magnesium sulfate, 0.0008% of iron sulfate, and the balance is water. 24 hours after cultivation, according to 10m 3 / h ventilation for aeration culture, after 24 hours of liquid culture, according to 15m 3 The aeration rate per hour was used for aeration culture, the temperature of the liquid culture proce...
Embodiment 3
[0072] The H. chlorogenic strain provided by Jiangxi Tianren Ecological Co., Ltd. was inoculated with 1.0% on the PDA solid slant medium, and activated at 30°C for 3 days to complete the activation of the strain. The activated H. chlorogenic strains were inoculated into a 250L fermenter equipped with a primary seed medium for liquid cultivation for 35 hours according to an inoculation amount of 1%. The culture temperature was 32° C., and the initial pH value was 5.5. The formula includes 2.00% of glucose, 0.50% of white sugar, 0.10% of soybean powder, 0.30% of ammonium sulfate, 0.05% of dipotassium hydrogen phosphate, 0.10% of potassium chloride, 0.01% of magnesium sulfate, and 0.0008% of iron sulfate. 24h after cultivation, according to 12m 3 / h ventilation for aeration culture, after 24 hours of liquid culture, according to 15m 3 The aeration rate per hour was used for aeration culture, the temperature of the liquid culture process was controlled at 30°C, the light intensit...
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