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Bacillus subtilis and its application

A technology of Bacillus subtilis and bacterial strains, applied in the field of Bacillus subtilis and its application, to achieve the effects of reduced fruit rot rate, good growth-promoting effect, and simple preparation process

Inactive Publication Date: 2008-02-13
LIAOCHENG SUBILIER FERTILIZER
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although many Bacillus subtilis biocontrol agents have been proven to have control effects, only a few are used in production, and most are still in the research and exploration stage

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Antagonism of Example 1 Bacillus subtilis CGMCC No.1982 to the main pathogenic bacteria in agricultural production

[0044] (1) Cultivation of antagonistic bacteria Take out the strain CGMCC No.1982 from the refrigerator at 4°C, place it for 2 hours, inoculate it by streaking on the LB plate, and culture it at 30°C for 2 days.

[0045](2) Cultivation of pathogenic fungi The preserved pathogenic fungi to be tested were inoculated on PDA plates, and cultured at 28°C to make them cover the plates. The pathogenic fungi tested included: Bipolaris maydis, Magnaporthe grisea, Fusarium graminearum, Fusarium oxysporum f.sp.Vasinfectum, and Pepper anthracnose ( Colletotrichum capsici), Phytophora capsici, Colletotrichum lagenarium, Sclerotinia allii, Verticillium dahliae, Alternaria alternata, Peach botrytis (Humicola Traaen) et al.

[0046] (3) Determination of Antagonism Spectrum When the mycelium of the pathogenic fungus is overgrown on the PDA plate, punch holes on the plat...

Embodiment 2

[0048] The control effect of the bacterial suspension of embodiment 2 Bacillus subtilis strain CGMCC No.1982 to rice blast

[0049] (1) Strain activation: TSA medium: tryptone 15g, soybean peptone 5g, NaCl 5g, water 1000mL;

[0050] After inoculating the preserved glycerol strain CGMCC No.1982 into TSA solid slant medium, culture it at 34°C for 48h;

[0051] (2) Seed culture: after rinsing the activated slant strains with sterile water, they were inoculated into a Erlenmeyer flask containing 40 mL of TSA liquid medium, with an inoculum volume of 1%. 160r / min, shaking culture at 34°C for 20h;

[0052] (3) Fermentation culture:

[0053] Fermentation medium: glucose 1%, yeast extract 1%, CaCO 3 1%, pH 7.5;

[0054] Fed-batch medium: 1.5% glucose, 0.5% yeast extract, 800mL mixture of glucose and yeast extract;

[0055] The seed solution was inoculated into the fermentation medium with an inoculum of 3% for fermentation and culture, and a 20L stirred fermenter was used for fed...

Embodiment 3

[0067] Example 3 The control effect of the bacterial suspension of Bacillus subtilis strain CGMCC No.1982 on corn leaf spot

[0068] The control effect of the bacterial suspension on maize spot bacterium was detected by the method substantially the same as that in Example 1. The results showed that the leaves of corn (B73C) treated with the bacterial suspension were not diseased; while the leaves of corn treated with clear water were all diseased, and the diameter of the lesion was 2×2mm on average. 2 .

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Abstract

The invention discloses a Bacillius subtilis strain, and the culture preservation code for the Bacillius subtilis at China General Microbiological Culture Collection Center (CGMCC) is CGMCC No.1982. Dry-powder microbial inoculum prepared by the strain can be applied to prevention and cure of wheat scab, rice blast, corn southern leaf blight, sclerotium disease germ of garlic, anthracnose germ of cucumber and other germs that cause diseases to agricultural crops and vegetables; the microbial inoculum can also be applied to preservation of fresh fruits such as peaches, apples, grapes, pears, etc. Rice and corn leaves processed with suspension liquid of Bacillus subtilis strain can prevent the corresponding lesion disease; the cucumber seeds or seedlings processed by mixing the seeds with powder, burying the powder around the root or spraying suspension liquid of Bacillus subtilis strain can not only prevent cucumber anthracnose, but also accelerate the growth of the plants. The production of safe and pollution-free vegetables is capable. Fresh peaches processed with suspension liquid of Bacillus subtilis strain are rot-resistance, and the preservation time is prolonged. The preparation process for the microbial inoculum is simple, applicable to commercial production.

Description

technical field [0001] The invention relates to a bacillus subtilis and the application of the bacterial agent thereof, belonging to a broad-spectrum biocontrol and growth-promoting bacterial agent. Background technique [0002] For more than half a century, the control of plant diseases and insect pests by chemical pesticides has played a very important role in agricultural production. However, with the emergence of problems such as pesticide residues, environmental pollution, and insect resistance caused by the long-term and extensive use of chemical pesticides, the safety of chemical pesticides has aroused widespread concern in the society, and biological control has become more and more popular. Pay attention to. Biological control has the advantages of being safe to humans and animals, non-toxic, and does not pollute the environment, and has gradually become one of the important measures for plant pest control. In the biological control of plant diseases and insect pe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02C12R1/125
Inventor 王立安成丽霞李小俊彭兵贾宇
Owner LIAOCHENG SUBILIER FERTILIZER
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