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Gene recombination bacillus amyloliquefaciens and microbial inoculum preparation method

A technology of amylolytic spores and gene recombination, applied in botany equipment and methods, methods based on microorganisms, biochemical equipment and methods, etc., can solve the problem of affecting the growth and colonization ability of bacteria, affecting the effect of disease prevention and growth promotion, etc. problem, to achieve the effect of high antibacterial lipopeptide production, good antibacterial effect and high genetic stability

Inactive Publication Date: 2012-05-23
HUNAN NORMAL UNIVERSITY
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Problems solved by technology

In addition, after being applied to the soil, if the oxygen content in the soil is low, it will affect the growth and colonization ability of the bacteria, and then affect its disease prevention and growth promotion effect

Method used

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  • Gene recombination bacillus amyloliquefaciens and microbial inoculum preparation method
  • Gene recombination bacillus amyloliquefaciens and microbial inoculum preparation method
  • Gene recombination bacillus amyloliquefaciens and microbial inoculum preparation method

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Embodiment Construction

[0056] The present invention will be described in further detail below in conjunction with the embodiments and accompanying drawings.

[0057] In the embodiment of the present invention, Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) FZB42, which promotes the growth and prevention of pathogens in the rhizosphere of plants, is used as the recipient bacterium to integrate the hemoglobin gene vgb of Vitiligo hyaline into the recombinant vector pDGP43-vgb. Achieving high expression in Bacillus amyloliquefaciens, constructing Bacillus amyloliquefaciens engineering bacteria strain XLV09-1 with higher spore number and antibacterial lipopeptide production, and preparing Bacillus amyloliquefaciens engineering bacteria strain.

[0058] 1. Example of the Construction Method of Gene Recombinant Bacillus amyloliquefaciens Engineering Bacteria

[0059] (1) PCR amplification of P43 promoter

[0060] Extract the total DNA of Bacillus subtilis B. subtilis 168 strain, method reference...

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Abstract

The invention discloses gene recombination bacillus amyloliquefaciens and a microbial inoculum preparation method. A bacillus amyloliquefaciens FZB42 strain is taken as a host bacterium to construct the gene recombination bacillus amyloliquefaciens XLV09-1 with transparent vitreoscilla hemoglobin which is regulated and controlled by a P43 strong promoter by contrasting a gene recombination vector. The produced spore number of microbial inoculum prepared by fermenting the bacillus amyloliquefaciens can reach more than 100 hundred millions per milliliter, the overall yield of antibacterial lipopeptid reaches more than 1 gram per liter and prevention effect on various fungal plant diseases is improved by over 30 percent. The microbial inoculum is a green microecological preparation, of no toxic residue, safe to human and livestock, and favorable for protecting the ecological environment.

Description

technical field [0001] The invention relates to a plant disease-resistant and growth-promoting engineering bacterium and a preparation method of a bacterial agent, in particular to a genetically recombined Bacillus amyloliquefaciens containing a hyaline hemoglobin gene and a preparation method of a bacterial agent. Background technique [0002] In agricultural production, the use of chemical pesticides and chemical fertilizers is increasing year by year, causing problems such as environmental pollution, ecological deterioration, soil compaction and soil degradation, and poses a huge threat and challenge to food safety, human health and sustainable agricultural development. Therefore, to develop sustainable agriculture, it is necessary to reduce the use of chemical pesticides and chemical fertilizers and find effective alternatives. [0003] Microbial pesticides and microbial fertilizers have the characteristics of long-lasting effect, no pollution to the environment, and low...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/21C12N15/31C12R1/07C12P21/02C12N15/75
Inventor 夏立秋丁学知刘清术余子全胡胜标孙运军
Owner HUNAN NORMAL UNIVERSITY
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