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Preparation method of bacillussubtilis 24-7 strain fermentation liquid

A technology of Bacillus subtilis and fermentation liquid, which is applied in the field of preparation of Bacillus subtilis 24-7 strain fermentation liquid, can solve the problems of unfavorable batch production and expansion, long production and fermentation period, complicated production process, etc. effect, shorten the fermentation cycle, and improve the effect of economic benefits

Inactive Publication Date: 2020-03-31
MICROBIAL FERMENTATION ENG RES CENT CO LTD OF YUNNAN PROVINCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the production process of the existing Bacillus subtilis preparation (i.e. the fermentation liquid of Bacillus subtilis) is complicated, the production fermentation cycle is long, and the production cost is high, which is unfavorable for the batch production and expansion of the Bacillus subtilis preparation

Method used

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preparation example Construction

[0010] The preparation method of Bacillus subtilis 24-7 bacterial strain fermentation liquid of the present invention comprises A, strain pretreatment, B, seed liquid preparation, C, fermentation liquid preparation, and concrete steps are

[0011] A. Strain pretreatment, draw the original Bacillus subtilis (Bacillus subtilis) 24-7 strain and inoculate it with the LB liquid medium contained in the inoculation container, and place the inoculation container after inoculation on a constant temperature shaker at 35°C and 160rpm Cultivate in medium for 72 hours, and finally sterilize with 50% glycerol and store the bacterial solution in a centrifuge tube at a ratio of 1:1 in an ultra-low temperature environment of -80°C;

[0012] B. Seed liquid preparation, first inoculate the Bacillus subtilis (Bacillus subtilis) 24-7 bacterial classification preserved in the centrifuge tube at -80°C on the LB solid medium plate, and mark on the LB solid medium plate in a 35°C constant temperature i...

Embodiment 1

[0021] The concrete steps of the preparation method of the Bacillus subtilis 24-7 bacterial strain fermentation liquid of the present invention are:

[0022] A. Strain pretreatment, draw the original Bacillus subtilis (Bacillus subtilis) 24-7 strain and inoculate it with the LB liquid medium contained in the inoculation container, and place the inoculation container after inoculation on a constant temperature shaker at 35°C and 160rpm Cultivate in medium for 72 hours, and finally sterilize with 50% glycerol and store the bacterial solution in a centrifuge tube at a ratio of 1:1 in an ultra-low temperature environment of -80°C;

[0023] B, preparation of seed liquid, first inoculate the Bacillus subtilis (Bacillus subtilis) 24-7 bacterial classification preserved in the centrifuge tube at -80°C on the LB solid medium plate, and the LB solid medium plate is streaked and cultivated in a 35°C constant temperature incubator 24h; then select a single colony of Bacillus subtilis (Bac...

Embodiment 2

[0028] The concrete steps of the preparation method of the Bacillus subtilis 24-7 bacterial strain fermentation liquid of the present invention are:

[0029] A. Strain pretreatment, draw the original Bacillus subtilis (Bacillus subtilis) 24-7 strain and inoculate it with the LB liquid medium contained in the inoculation container, and place the inoculation container after inoculation on a constant temperature shaker at 35°C and 160rpm Cultivate in medium for 72 hours, and finally sterilize with 50% glycerol and store the bacterial solution in a centrifuge tube at a ratio of 1:1 in an ultra-low temperature environment of -80°C;

[0030]B, preparation of seed liquid, first inoculate the Bacillus subtilis (Bacillus subtilis) 24-7 bacterial classification preserved in the centrifuge tube at -80°C on the LB solid medium plate, and the LB solid medium plate is streaked and cultivated in a 35°C constant temperature incubator 24h; then select a single colony of Bacillus subtilis (Baci...

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Abstract

The invention discloses a preparation method of bacillussubtilis 24-7 strain fermentation liquid. The preparation method comprises steps of strain pretreatment, seed liquid preparation and fermentation liquid preparation. The bacillussubtilis 24-7 strain fermentation liquid contains active substances of subtilin, nystatin and gramicidin and the active substances have an obvious inhibition effect on pathogenic bacteria such as cucumber powdery mildew, tomato powdery mildew and tobacco powdery mildew. The method is safe, non-toxic and residue-free; and the environment is not polluted. With the fermentation production process of bacillussubtilis 24-7, the spore content of the fermentation liquid can be improved, so that the biocontrol effect of the preparation is improved; the raw materials are reasonably proportioned, so that the utilization rate is maximized and the fermentation period is shortened; the method is suitable for realizing batch production; and thus the operation cost of manpower, equipment, water, electricity, gas and the like is reduced and the economic benefit is finally increased.

Description

technical field [0001] The invention belongs to the technical field of preparation of bacillus fermentation liquid, and in particular relates to a preparation method of fermentation liquid of bacillus subtilis 24-7 strain. Background technique [0002] Bacillus subtilis (Bacillus subtilis) is a mesophilic, aerobic, spore-forming, Gram-positive rod-shaped bacterium, which has the functions of promoting crop growth, inhibiting plant pathogenic bacteria, and accelerating the composting process of livestock manure and daily waste compost. , is an important functional bacteria for the preparation of organic waste composting fermentation bacteria or bio-organic fertilizer. As a biocontrol bacterium, Bacillus subtilis has broad development prospects, and has been widely used in agricultural biocontrol, animal husbandry and breeding, medicine and molecular biology research. Today's chemical pesticides play a very important role in agricultural production, but the continuous and unr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N3/00C12P1/04A01P3/00C12R1/125
CPCC12N1/20C12N3/00C12P1/04C12N1/205C12R2001/125
Inventor 郭绍青何月秋赵崇钧李诚林丹陈松涛王林涛李建云周宗贵
Owner MICROBIAL FERMENTATION ENG RES CENT CO LTD OF YUNNAN PROVINCE
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