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Preparation method for bacillus methylotrophicus wettable powder

A methylotrophic, wettable powder technology, applied in botany equipment and methods, biocides, animal repellants, etc., can solve the problem of spore germination agent and spore germination of methylotrophic bacillus that have not been publicly reported. It can improve the germination rate and germination rate, reduce the probability of germination, and reduce the amount of spores due to the problems of low humidity and short wetting time.

Active Publication Date: 2016-05-11
FOSHAN YANHUI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0016] At the present stage, there is no public report on the spore germination agent of methylotrophic bacillus, and there is no report on the research of adding a spore germination agent to the wettable powder of methylotrophic bacillus
[0017] In addition, due to the compatibility of different bacillus with wetting agents, dispersants and other additives, the carrier has different adsorption properties to various bacillus, and the existing formula or preparation process of bacillus wettable powder cannot be used Prepare methylotrophic bacillus wettable powder containing spore germination agent; the adsorption amount of effective bacteria in wettable powder, survival rate, control effect on harmful bacteria and stability of wettable powder dose are quite different, the whole can be The preparation method of wet powder must fully consider the adsorption capacity of each carrier, the protective effect on the drying process of methylotrophic bacillus, the influence of wetting agent and dispersant on the suspension rate of wettable powder, the wetting time, and the influence on the spore germination agent. Comprehensive screening can obtain the wettable powder of methylotrophic bacillus with short wetting time, high suspension rate, long storage time of spores, rapid germination of spores after use, and good control effect.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] A methylotrophic bacillus wettable powder consists of the following parts by weight: 60 parts of methylotrophic bacillus fermentation broth; filler: 26 parts of talcum powder; wetting agent: 3 parts of sodium dodecylbenzenesulfonate ; Dispersant: 0.7 parts of tea saponin; Spore germination agent: 0.4 parts of methionine, 0.2 parts of calcium 2,6-pyridine dicarboxylate; UV protection agent: 1.5 parts of ascorbic acid, 0.6 parts of β-dextrin; Drying protection agent: maltose 3 parts, mannitol 0.8 part.

[0043] A method for preparing a methylotrophic bacillus wettable powder, comprising the following steps:

[0044] 1. Preparation of Methylotrophic Bacillus Fermentation Broth

[0045] Take out the strain preservation tube, scratch the plate with NA solid medium for recovery, and incubate at 30°C for 48 hours. Pick a single colony under the plate and inoculate it on the NA solid medium in the eggplant bottle, spread it all over the eggplant bottle, and culture it in the ...

Embodiment 2

[0053] The spore germination test of embodiment 2 methylotrophic bacillus wettable powder

[0054] After adding a germination agent to the spore germination system, the spores began to germinate, and the optical density of the system at 600nm decreased with the increase of the germination rate (the spores have high refraction, and the light absorption after germination is enhanced), when the germination rate reaches 100% , the optical density decreases to 50-60% of the initial value, and the decrease in optical density within this range is inversely proportional to the increase in spore germination rate, that is, the germination rate of spores is twice the decrease rate of optical density. The germination rate of spores can be calculated by measuring the decrease rate of the spore system at 600nm optical density.

[0055]The prepared methylotrophic bacillus wettable powder and water were wetted and mixed evenly at a ratio of 1:1000. After 30 minutes at 30°C, the spore germinat...

Embodiment 3

[0057] A methylotrophic bacillus wettable powder consists of the following parts by weight: 50 parts of methylotrophic bacillus fermentation broth; filler: 22 parts of talcum powder; wetting agent: 2 parts of sodium dodecylbenzenesulfonate ; Dispersant: 0.4 part of tea saponin; Spore germination agent: 0.2 part of methionine, 0.2 part of calcium 2,6-pyridinedicarboxylate; UV protection agent: 0.8 part of ascorbic acid, 0.6 part of β-dextrin; Drying protection agent: maltose 2 parts, mannitol 0.5 part.

[0058] A method for preparing a methylotrophic bacillus wettable powder, comprising the following steps:

[0059] 1. Preparation of Methylotrophic Bacillus Fermentation Broth

[0060] Take out the strain preservation tube, scratch the plate with NA solid medium for recovery, and incubate at 30°C for 48 hours. Pick a single colony under the plate and inoculate it on the NA solid medium in the eggplant bottle, spread it all over the eggplant bottle, and culture it in the incuba...

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Abstract

The invention relates to the field of biopesticides and microbial fertilizers and particularly discloses bacillus methylotrophicus wettable powder containing a spore germination agent and a preparation method thereof. The wettable powder is prepared from the following raw materials in parts by weight: 40-70 parts of bacillus methylotrophicus, 20-30 parts of talcum powder serving as a padding, 1-4 parts of sodium dodecyl benzene sulfonate serving as a wetting agent, 0.2-1 part of tea saponin serving as a dispersing agent, 0.1-0.5 part of methionine and 0.1-0.5 part of 2,6-dipicolinic acid calcium salt serving as a spore germinator, 0.5-2 parts of ascorbic acid and 0.2-1 part of beta-dextrin serving as an ultraviolet protector, and 1-4 parts of maltose and 0.2-1 part of mannitol serving as a dryness protector. After the bacillus methylotrophicus wettable powder prepared by the preparation method is applied to a land for growing field crops, the spore germination speed is rapid, and the germination rate is high; and the bacillus methylotrophicus wettable powder is capable obviously improving the fertilizer efficiency and plant disease control effect, reducing the spore dosage and lowering the cost.

Description

technical field [0001] The invention relates to the fields of biological pesticides and microbial fertilizers, in particular to a methylotrophic Bacillus wettable powder containing a spore germination agent and a preparation method thereof. Background technique [0002] my country uses a large amount of chemical pesticide preparations and fertilizers every year, which can easily cause excessive pesticide residues and bio-enrichment, which not only pollutes the ecological environment, but also affects human health. However, biopesticide has few side effects and is harmless to humans. It is a kind of green pesticide with broad prospects and has increasingly become a trend in the development of global pesticides. [0003] Bacillus is currently the most researched microorganism in biological pesticides and microbial fertilizers. It has broad-spectrum antibacterial activity and strong resistance to stress. Its vegetative cells can reproduce and colonize on the surface of fungal p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02A01N43/40A01N37/44A01N25/14A01P21/00
CPCA01N25/14A01N37/44A01N43/40A01N63/10A01N2300/00
Inventor 胡艳晖
Owner FOSHAN YANHUI BIOTECH CO LTD
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