Preparation method for bacillus methylotrophicus wettable powder
A methylotrophic, wettable powder technology, applied in botany equipment and methods, biocides, animal repellants, etc., can solve the problem of spore germination agent and spore germination of methylotrophic bacillus that have not been publicly reported. It can improve the germination rate and germination rate, reduce the probability of germination, and reduce the amount of spores due to the problems of low humidity and short wetting time.
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Embodiment 1
[0042] A methylotrophic bacillus wettable powder consists of the following parts by weight: 60 parts of methylotrophic bacillus fermentation broth; filler: 26 parts of talcum powder; wetting agent: 3 parts of sodium dodecylbenzenesulfonate ; Dispersant: 0.7 parts of tea saponin; Spore germination agent: 0.4 parts of methionine, 0.2 parts of calcium 2,6-pyridine dicarboxylate; UV protection agent: 1.5 parts of ascorbic acid, 0.6 parts of β-dextrin; Drying protection agent: maltose 3 parts, mannitol 0.8 part.
[0043] A method for preparing a methylotrophic bacillus wettable powder, comprising the following steps:
[0044] 1. Preparation of Methylotrophic Bacillus Fermentation Broth
[0045] Take out the strain preservation tube, scratch the plate with NA solid medium for recovery, and incubate at 30°C for 48 hours. Pick a single colony under the plate and inoculate it on the NA solid medium in the eggplant bottle, spread it all over the eggplant bottle, and culture it in the ...
Embodiment 2
[0053] The spore germination test of embodiment 2 methylotrophic bacillus wettable powder
[0054] After adding a germination agent to the spore germination system, the spores began to germinate, and the optical density of the system at 600nm decreased with the increase of the germination rate (the spores have high refraction, and the light absorption after germination is enhanced), when the germination rate reaches 100% , the optical density decreases to 50-60% of the initial value, and the decrease in optical density within this range is inversely proportional to the increase in spore germination rate, that is, the germination rate of spores is twice the decrease rate of optical density. The germination rate of spores can be calculated by measuring the decrease rate of the spore system at 600nm optical density.
[0055]The prepared methylotrophic bacillus wettable powder and water were wetted and mixed evenly at a ratio of 1:1000. After 30 minutes at 30°C, the spore germinat...
Embodiment 3
[0057] A methylotrophic bacillus wettable powder consists of the following parts by weight: 50 parts of methylotrophic bacillus fermentation broth; filler: 22 parts of talcum powder; wetting agent: 2 parts of sodium dodecylbenzenesulfonate ; Dispersant: 0.4 part of tea saponin; Spore germination agent: 0.2 part of methionine, 0.2 part of calcium 2,6-pyridinedicarboxylate; UV protection agent: 0.8 part of ascorbic acid, 0.6 part of β-dextrin; Drying protection agent: maltose 2 parts, mannitol 0.5 part.
[0058] A method for preparing a methylotrophic bacillus wettable powder, comprising the following steps:
[0059] 1. Preparation of Methylotrophic Bacillus Fermentation Broth
[0060] Take out the strain preservation tube, scratch the plate with NA solid medium for recovery, and incubate at 30°C for 48 hours. Pick a single colony under the plate and inoculate it on the NA solid medium in the eggplant bottle, spread it all over the eggplant bottle, and culture it in the incuba...
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