Fungicide containing bacillus and application thereof
A technology of bacillus and inoculum, applied in the field of microorganisms, can solve problems such as pollution of the environment, and achieve the effects of high bioavailability, long shelf life, and improved yield and quality
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Embodiment 1
[0023] This example describes the use of rice husks, sawdust, and wheat husks as carriers to prepare Bacillus amyloliquefaciens ( bacillus amyloliquefaciens ) live bacterial preparation of K-8. Bacillus amyloliquefaciens ( bacillus amyloliquefaciens ) K-8 is abbreviated as strain K-8.
[0024] 1. Rice husk, sawdust, wheat husk as carrier
[0025] Fresh, mildew-free, lump-free, insect-free, loose and dry rice husks or wheat husks are selected as raw materials, mechanically crushed and passed through a vibrating sieve with an aperture of 2 mm to obtain rice husk or wheat husk carriers.
[0026] Select fresh, mildew-free, lump-free, insect-free, loose and dry sawdust to directly pass through a vibrating sieve with an aperture of 2 mm to obtain a sawdust carrier.
[0027] The above-mentioned carriers may be used alone or in combination. It is used alone in the present embodiment 1.
[0028] 2. Preparation of strain K-8 fermentation broth
[0029] The preparation of bacterial...
Embodiment 2
[0053]This example introduces the selection of fermented and decomposed substrates as carriers for preparing pigs containing Bacillus amyloliquefaciens ( bacillus amyloliquefaciens ) live bacterial preparation of K-8.
[0054] 1. Acquisition of carrier
[0055] The live bacteria adsorption carrier used in this example comes from the fermentation bed used in raising pigs.
[0056] Fermentation bed technology (also known as the lazy man pig raising method) is an emerging environmentally friendly pig raising technology, which can solve the huge pressure on the environment (mainly waste water and manure) brought by the pig industry, and can reduce environmental pollution without increasing investment. It can also reduce labor intensity, cultivate healthy and disease-free live pigs, and significantly improve economic benefits.
[0057] The substrate used on the fermentation bed can be composed of one or two or more of rice husk, sawdust, wheat husk, leaves, weeds and straw (cut i...
Embodiment 3
[0078] Adopt the bacterium agent or carrier prepared in embodiment 1 and embodiment 2, carry out tomato planting field test in a greenhouse in the suburb of Binhai County, Yancheng City, Jiangsu Province (sowing on February 18, 2013, transplanting to greenhouse on April 20, The distance between plants is 30 cm, about 3,000 plants are planted per mu, each greenhouse is 0.245 mu, divided into 2 ridges, each ridge is 0.1225 mu, and each ridge has 2 test plots, that is, the test area of each plot is 0.06125 mu). Among them, the F-4 test field uses the Fubang brand bio-organic fertilizer produced by Angel Yeast Co., Ltd. The specific experimental design is shown in Table 4.
[0079] Table 4 Field experiment design table
[0080] Test Field No. fertilizer Remark F-1 Bacteria agent 1 in embodiment 1 F-2 Fermentation bed carrier in embodiment 2 Unmixed strain K-8 fermentation broth F-3 Bacteria agent 4 in embodiment 2 F-4 Water-soluble ...
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