Bacillus subtilis and its application
A technology of Bacillus subtilis and strains, applied in the field of Bacillus subtilis and its application, to achieve good application prospects, reduce rotten fruit rate, and prolong storage period
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0043] Antagonism of Example 1 Bacillus subtilis CGMCC No.1982 to the main pathogenic bacteria in agricultural production
[0044] (1) Cultivation of antagonistic bacteria Take the strain CGMCC No.1982 from the refrigerator at 4°C, put it aside for 2 hours, inoculate it by streaking on the LB plate, and culture it at 30°C for 2 days.
[0045](2) Cultivation of pathogenic fungi The preserved pathogenic fungi to be tested were inoculated on PDA plates, and cultured at 28°C to make them cover the plates. The pathogenic fungi tested included: Bipolaris maydis, Magnaporthe grisea, Fusarium graminearum, Fusarium oxysporum f.sp.Vasinfectum, and Pepper anthracnose ( Colletotrichum capsici), Phytophora capsici, Colletotrichum lagenarium, Sclerotinia allii, Verticillium dahliae, Alternaria alternata, Peach botrytis (Humicola Traaen) et al.
[0046] (3) Determination of Antagonism Spectrum When the mycelium of the pathogenic fungus is overgrown on the PDA plate, punch holes on the plat...
Embodiment 2
[0048] The control effect of the bacterial suspension of embodiment 2 Bacillus subtilis strain CGMCC No.1982 to rice blast
[0049] (1) Strain activation: TSA medium: tryptone 15g, soybean peptone 5g, NaCl 5g, water 1000mL;
[0050] After inoculating the preserved glycerol strain CGMCC No.1982 into TSA solid slant medium, culture it at 34°C for 48h;
[0051] (2) Seed culture: after rinsing the activated slant strains with sterile water, they were inoculated into a Erlenmeyer flask containing 40 mL of TSA liquid medium, with an inoculum volume of 1%. 160r / min, shaking culture at 34°C for 20h;
[0052] (3) Fermentation culture:
[0053] Fermentation medium: glucose 1%, yeast extract 1%, CaCO 3 1%, pH7.5;
[0054] Fed-batch medium: 1.5% glucose, 0.5% yeast extract, 800mL mixture of glucose and yeast extract;
[0055] The seed solution was inoculated into the fermentation medium with an inoculum of 3% for fermentation and culture, and a 20L stirred fermenter was used for fed-...
Embodiment 3
[0067] Example 3 The control effect of the bacterial suspension of Bacillus subtilis strain CGMCC No.1982 on corn leaf spot
[0068] The control effect of the bacterial suspension on maize spot bacterium was detected by the method substantially the same as that in Example 1. The results showed that the leaves of corn (B73C) treated with the bacterial suspension were not diseased; while the leaves of corn treated with clear water were all diseased, and the diameter of the lesion was 2×2mm on average. 2 .
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com