Brevibacillus brevis liquid-submerged fermentation method and wettable powder preparation method
A technology of Bacillus brevis, liquid submerged fermentation, applied in the methods of using spores, fungicides, botanical equipment and methods, etc., can solve problems such as troubles, achieve strong resistance to stress, improve the level of comprehensive utilization, and increase economy. effect of benefit
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Embodiment 1
[0030] Preparation of Bacillus brevis wettable powder:
[0031] (1) Strain activation: Streak inoculation of Bacillus brevis on a nutrient agar medium plate for culture, culture at 30°C for 24h, then pick a single colony and transfer it to the slant of nutrient agar, culture at 30°C for 24h Afterwards, the activated bacteria were obtained.
[0032] (2) Preparation of primary seed liquid in shake flasks: 500 mL of seed culture medium containing 5 g of yeast extract, 1 g of peptone, 1 g of glucose, and pH 7.0 ± 0.1 were respectively loaded into two 2000 mL conical flasks, and the The activated strains were inoculated into the shake flask seed medium with an inoculation loop, and the culture conditions were as follows: temperature 36°C, shaker speed 160r / min, culture time 12h.
[0033] (3) Preparation of secondary seed liquid in seed tank: After the seed liquid obtained in step (2) is microscopically inspected without contamination by bacteria, insert 500 g of yeast extract, 100...
Embodiment 2
[0041] Preparation of Bacillus brevis wettable powder:
[0042] (1) Strain activation is the same as in Example 1.
[0043] (2) The preparation of the primary seed solution of the shake flask is the same as in Example 1.
[0044] (3) The preparation of the secondary seed liquid in the seed tank is the same as in Example 1.
[0045] (4) Fermentation tank high-density fermentation culture: the seed liquid gained in the step (3) is inserted in the 1000L fermentation tank by the inoculum size of 1% after microscopic examination without assorted bacteria pollution, and the fermentation medium composition in the tank: sucrose molasses 10g / L, soybean flour 5g / L, peptone 2g / L, MgSO 4 0.5g / L, CaCO 3 4g / L, liquid volume in fermentation tank 500L, culture conditions: temperature 30±2°C, stirring rate 160r / min, ventilation volume 15m 3 / h, tank pressure 0.05Mpa, when more than 90% thalline form in the fermented liquid is spore, stop fermentation, get the fermented liquid for subsequent ...
Embodiment 3
[0052] Preparation of Bacillus brevis wettable powder:
[0053] (1) Strain activation: same as in Example 1.
[0054] (2) Preparation of primary seed solution in shake flask: same as in Example 1.
[0055] (3) Preparation of the secondary seed liquid in the seed tank: same as in Example 1.
[0056] (4) Fermentation tank high-density fermentation culture: the seed liquid gained in the step (3) is inserted in the 1000L fermentation tank by the inoculum size of 5% after microscopic examination without assorted bacteria pollution, and the fermentation medium composition in the tank: sucrose molasses 30g / L, soybean flour 15g / L, peptone 6g / L, MgSO 4 3.5g / L, CaCO 3 8g / L, liquid volume in fermentation tank 700L, culture conditions: temperature 30±2°C, stirring rate 200r / min, ventilation volume 25m 3 / h, tank pressure 0.06Mpa, when more than 90% thalline form in the fermented liquid is spore, stop fermentation, get the fermented liquid for subsequent use, and the fermentation cycle...
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