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Lactalbumin polypeptide with antioxidation activity and preparation method thereof

A technology of whey protein peptides and antioxidant activity, which is applied in the fields of hydrolyzed protein components, animal protein processing, peptides, etc., can solve the problems such as no reports of whey protein antioxidant peptides, and achieve an improved level and high free radical scavenging , the effect of improving the utilization rate

Inactive Publication Date: 2009-09-02
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Foreign related anti-oxidation reports are based on the iron-catalyzed lecithin lipid oxidation system, the copper-catalyzed lipid oxidation system, and the use of whey protein α-lactalbumin and β-lactoglobulin to prepare antioxidant peptides. oxidized peptide report

Method used

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  • Lactalbumin polypeptide with antioxidation activity and preparation method thereof
  • Lactalbumin polypeptide with antioxidation activity and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] After the whey protein substrate with a concentration of 5% is heat-treated at a temperature of 95° C. for 5 minutes, the temperature is lowered to 60° C., and alkaline protease is added for hydrolysis. The concentration ratio of alkaline protease to whey protein substrate [ E / S] is 2:100, hydrolyzed for 5 hours at 60°C and pH 8.5 (the degree of hydrolysis (DH) reached 35.30% after testing); the hydrolyzate was boiled to kill the enzyme, cooled and concentrated , freeze-dried, that is. After testing, the prepared whey protein polypeptides are mainly composed of mixed whey protein polypeptides with a molecular weight between 100 and 400,000.

Embodiment 2

[0031] After the whey protein substrate with a concentration of 5% is heat-treated at a temperature of 95° C. for 5 minutes, the temperature is lowered to 60° C., and alkaline protease is added for hydrolysis. The concentration ratio of alkaline protease to whey protein substrate [ E / S] is 2:100, under the conditions of 55 ℃ temperature and pH value 8.5, hydrolyze for 5 hours (after testing, the degree of hydrolysis (DH) reaches 35.30%); the hydrolyzate is boiled, cooled, concentrated, after Sephadex G-10 gel chromatography separation and purification obtained a group of antioxidant peptide products with a molecular weight between 100-2800Da; After purification by reverse-phase high-performance liquid chromatography, a single peak of a peptide with higher activity was obtained, and the mass spectrometry (MS) measurement result showed that it was a mixed peptide composed of peptides with a molecular weight of 592.0; the peptide sequence is: Val-His- Leu-Lys-Pro.

[0032] The u...

Embodiment 3

[0037] After the whey protein substrate with a concentration of 5% is heat-treated at a temperature of 95° C. for 5 minutes, the temperature is lowered to 55° C., and alkaline protease is added for hydrolysis. The concentration ratio of alkaline protease to whey protein substrate [ E / S] is 3:100, hydrolyzed for 6 hours at a temperature of 55°C and a pH value of 8.0; the hydrolyzate is boiled, cooled, concentrated, and freeze-dried to obtain the product.

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Abstract

The invention discloses a preparation method of lactalbumin polypeptide with antioxidation activity and a product therefrom. The preparation method comprises the following steps: (1) heating lactalbumin; (2) cooling the heated lactalbumin, and adding the cooled lactalbumin in alkali protease for hydrolysis; and (3) obtaining mixed polypeptide which has strongest antioxidation activity with peptide ranging from 100-2800 Da after enzyme inactivation, temperature reduction, concentration and purification by SephadexG-10 gel chromatography column are carried out for the product of the hydrolysis. The prepared lactalbumin polypeptide has higher antioxidation activity and the capability of removing free radical as well as favorable antioxidation both in vitro and in vivo. The preparation method has simple operation, is safe and reliable compared with synthetic antioxidant, is suitable for the commercial process, and can increase the utilization rate of lactalbumin resources substantially and enhance the multipurpose use level of whey.

Description

technical field [0001] The invention relates to a polypeptide product and a preparation method thereof, in particular to a whey protein polypeptide with antioxidant activity and a preparation method thereof, belonging to the field of antioxidant polypeptide products. Background technique [0002] Whey is the liquid part separated from cow's milk when producing cheese and casein. The content of whey protein is about 13% of the dry weight. The composition of essential amino acids in whey protein fully meets or exceeds the requirements of FAO / WHO. Compared with other proteins, it has the highest bioavailability value. At present, the development of functional peptides using different protein resources has become a research hotspot for scientists at home and abroad. [0003] Whey protein is extracted from milk, which is 87% water and 13% milk solids. In milk solids, 27% is milk protein, only 20% of milk protein is whey protein, and the remaining 80% is casein. Therefore, the c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K2/00C07K7/06C12P21/06A61K38/01A61P39/06A23J3/08
Inventor 孔保华彭新颜李艾黎刁新平
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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